Improved cryopreservation of domestic cat sperm in a chemically defined medium

Vick, Mandi M.; Bateman, Helen L.; Lambo, C. A.; Swanson, William F.

doi:10.1016/j.theriogenology.2012.08.009

Cited by 36 publications

(24 citation statements)

References 46 publications

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“…For sperm cryopreservation, the washed sperm pellet was extended in TES‐Tris buffer with 1% soy lecithin (SOY), with 4% glycerol added after cooling to 5°C (Vick et al. ). Sperm samples (50 μl; motile sperm concentration, 80 × 10 6 /ml) were drawn up into 0.25‐cc semen straws and heat sealed.…”

Section: Methodsmentioning

confidence: 99%

“…Slides were then processed with FITC‐PNA stains and examined using fluorescent microscopy (Vick et al. ). To assess motility of fresh vs frozen sperm over time, aliquots of diluted semen from AI samples were centrifuged (600 g, 8 min) and sperm pellets resuspended in FOCM‐IVF medium to 10 × 10 6 motile sperm/ml.…”

Section: Methodsmentioning

confidence: 99%

“…Samples from each microdrop were evaluated for percent motility and progressive forward movement at 0‐ , 1‐ , 3‐ , 6‐ and 24‐h post‐‐insemination (Vick et al. ).…”

Section: Methodsmentioning

confidence: 99%

“…In concurrent research, we have found that freezing cat spermatozoa in a soy lecithin–based cryoprotectant medium appear to improve post‐‐thaw sperm motility and longevity over the standard egg yolk–based cryoprotectant, while showing comparable fertility in vitro (Vick et al. ). In the current study, our aim was to combine these two research paths and investigate pregnancy outcomes following LO‐AI with semen frozen in a soy lecithin–based medium.…”

Section: Introductionmentioning

confidence: 99%

“…With frozen semen, cryopreservation typically results in the loss of sperm motility and viability, especially in cats that produce high proportions of abnormal sperm (Pukazhenthi and Wildt ), and inclusion of animal proteins, such as egg yolk, in freezing media creates additional regulatory, disease and batch consistency concerns (Vick et al. ).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Fertility of Freshly Collected vs Frozen–Thawed Semen with Laparoscopic Oviductal Artificial Insemination in Domestic Cats

Lambo

Grahn

Lyons

et al. 2012

Reprod Domestic Animals

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ContentsArtificial insemination (AI) is potentially invaluable as an adjunct to natural breeding for the conservation management of non-domestic felid populations. The efficacy of AI, however, must be substantially improved for applied use, especially when using frozen semen. Our recent advances in using laparoscopic oviductal AI (LO-AI) with low sperm numbers and freezing of cat semen in a soy lecithin-based cryoprotectant medium suggest that combining these two approaches might improve pregnancy outcomes with frozenthawed spermatozoa. In this study, our objectives were to (i) assess the effect of two gonadotropin dosages (100 vs 150 IU eCG) on ovarian response in domestic cats and (ii) compare the relative fertility of frozen-thawed and fresh semen in vivo following LO-AI. All 16 females ovulated after gonadotropin treatment and were inseminated with fresh semen from one male and frozen-thawed semen from a second male. There were no differences between gonadotropin dosages in CL number, pregnancy percentage or litter size. Half (8/16) of the females conceived, with seven females giving birth to a total of 36 offspring. Paternity analysis showed that more kittens resulted from LO-AI with fresh (28/36, 78%) than frozenthawed (8/36, 22%) semen, possibly due to impaired motility and longevity of thawed sperm. These results demonstrated that viable offspring can be produced by AI using semen frozen in a soy lecithin-based medium. Insemination with greater numbers of frozen-thawed spermatozoa, combined with further refinement of cat sperm cryopreservation methods, may be necessary to optimize pregnancy success with LO-AI in domestic and nondomestic cats.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Samples from each microdrop were evaluated for percent motility and progressive forward movement at 0‐ , 1‐ , 3‐ , 6‐ and 24‐h post‐‐insemination (Vick et al. ).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparative Fertility of Freshly Collected vs Frozen–Thawed Semen with Laparoscopic Oviductal Artificial Insemination in Domestic Cats

Lambo

Grahn

Lyons

et al. 2012

Reprod Domestic Animals

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Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

Nguyen

Ponchunchoovong

Kupittayanant

et al. 2019

Veterinary Medicine & Sci

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The reduction of spermatozoa survival time is a major problem of canine chilled sperm for artificial insemination. The aim of the study was to improve the quality of canine chilled sperm during storage time. We therefore, evaluated the effects of eight treatments with different levels of soybean lecithin concentration (1, 3 and 5%) and egg yolk (20%) in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender on chilled canine sperm quality during 10 days of storage. The sperm motility was analysed by computer‐assisted sperm analysis (CASA), whereas plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential parameters were determined using a fluorescent staining combination of propidium iodide (PI), Hoechst 33342 (H342), fluorescein isothiocyanate‐conjugated Pisum sativum agglutinin (FITC‐PSA) and 5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethylbenzimidazolyl‐carbocyanine iodide (JC‐1) by confocal laser scanning microscope. The results showed that egg yolk was found to be better than soybean lecithin in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender for maintaining the quality of chilled canine sperm within 10 days of storage (P < 0.05). Although egg yolk in Tris‐citric‐fructose extender could maintain the motility better than other extenders, egg yolk in Tris‐citric‐fructose‐mineral salts extender was the highest in intact plasma membrane, intact acrosome membrane and high mitochondrial membrane potential (P < 0.05). In contrast, the sperm quality of soybean lecithin in Tris‐citric‐fructose‐mineral salts extender was lower than that of soybean lecithin in Tris‐citric‐fructose extender, and soybean lecithin 1% was greater than soybean lecithin 3% and 5% in plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential (P < 0.05). In conclusion, soybean lecithin cannot replace egg yolk in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extenders, and egg yolk in Tris‐citric‐fructose‐mineral salts extender is superior to other extenders in chilling canine sperm.

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Cryopreservation of spermatozoa from black-and-gold howler monkeys (Alouatta caraya) using egg yolk-based or soy lecithin-based extenders

Burch,

Nichi,

Mendes

et al. 2024

Primates

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Improved cryopreservation of domestic cat sperm in a chemically defined medium

Cited by 36 publications

References 46 publications

Comparative Fertility of Freshly Collected vs Frozen–Thawed Semen with Laparoscopic Oviductal Artificial Insemination in Domestic Cats

Comparative Fertility of Freshly Collected vs Frozen–Thawed Semen with Laparoscopic Oviductal Artificial Insemination in Domestic Cats

Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

Cryopreservation of spermatozoa from black-and-gold howler monkeys (Alouatta caraya) using egg yolk-based or soy lecithin-based extenders

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