Improved cellular response on functionalized polypyrrole interfaces

Abstract

Neuroregeneration strategies involve multiple factors to stimulate nerve regeneration. Neural support with chemical and physical cues to optimize neural growth and replacing the lesion neuron and axons are crucial for designing neural scaffolds, which is a promising treatment approach. In this study, polypyrrole polymerization and its functionalization at the interface developed by glycine and gelatin for further optimization of cellular response. Nanofibrous scaffolds were fabricated by electrospinning of polyvinyl alcohol and chitosan solutions. The electrospun scaffolds were polymerized on the surface by pyrrole monomers to form an electroactive interface for further applications in neural tissue engineering. The polymerized polypyrrole showed a positive zeta potential value of 57.5 ± 5.46 mV. The in vitro and in vivo biocompatibility of the glycine and gelatin‐functionalized polypyrrole‐coated scaffolds were evaluated. No inflammatory cells were observed for the implanted scaffolds. Further, DAPI nucleus staining showed a superior cell attachment on the gelatin‐functionalized polypyrrole‐coated scaffolds. The topography and tuned positively charged polypyrrole interface with gelatin functionalization is expected to be particularly efficient physical and chemical simultaneous factors for promoting neural cell adhesion.

Investigation of 3D-printed chitosan-xanthan gum patches

“…The swelling behaviour indicated increased water uptake with increasing XG content in the patches. Consequently, it is expected that this study may have application for skin tissue engineering or dressing for wound healing considering CH4/XG (1) patches are optimised to be used for that purpose and also can be improved by further studies. Both material optimisation and field characteristics will be considered.…”

Conductive Polymer‐Based Bioelectronic Platforms toward Sustainable and Biointegrated Devices: A Journey from Skin to Brain across Human Body Interfaces

“…[68] In bioelectronics polypyrrole (PPy) and polyaniline (PANI) (Figure 2b) are extensively employed polymers featuring remarkable antimicrobial effects, besides combining mixed ion-proton conductivity and redox activity typical of CPs. [69,70] Moreover, CPs physical and chemical properties can be easily tuned by introducing chemical moieties on the polymer backbone to enhance their electronic performance or bind molecules of interest for bio-detection [71] allowing the development of stimuli responsive biomedical platforms for biosensing, modulation of cellular activity, [72,73] drug-delivery systems, [74][75][76][77] tissue engineering, [78][79][80][81] and nerve regeneration applications. [82] For instance, the immobilization of specific enzymes has allowed the detection of biomarkers in epidermal [83,84] electrochemical sensors while the use of amide chemistry, by linking different biofunctional groups to poly(phenylene) ethynylene polymer (PPE), has enabled pH detection and flow cytometry applications.…”

Electroconductive multi-functional polypyrrole composites for biomedical applications

“…The chitosan protected the polypyrrole nanocomposite against corrosion, which is important for implantation devices [71] . Surface modification of polypyrrole nanocomposites by gelatin prevented inflammation upon implantation besides improving the cell attachment capacity of polypyrrole scaffolds [72] .…”