Improve freezing tolerance in transgenic poplar by overexpressing a ω-3 fatty acid desaturase gene

Zhou, Zhigang; Wang, M.-J.; Hu, Jianjun; Lu, Mengzhu; Wang, J. H.

doi:10.1007/s11032-009-9352-1

Cited by 12 publications

(4 citation statements)

References 36 publications

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“…Leaves of 15- to 25-day-old plantlets K326 clone at tissue culture stage were used as explants for transformation. A. tumefaciens strain LBA4404 harboring the pBin438-TAT-HezK I , pBin438-CpTI , or pBin438-TAT-HezK I-CpTI were used to transform K326 via the leaf-disc method [ 20 ]. Briefly, infected leaf discs were grown in MS basal medium supplemented with 1.0 mg/L of 6-benzyl aminopurine (6-BA) and 0.1 mg/L of naphthaleneacetic acid (NAA) in the dark for two days at 25 °C, and then transferred on the same medium with 50 mg/L kanamycin under a 16 h/8 h light/dark regime.…”

Section: Methodsmentioning

confidence: 99%

Transgenic Tobacco Expressing the TAT-Helicokinin I-CpTI Fusion Protein Show Increased Resistance and Toxicity to Helicoverpa armigera (Lepidoptera: Noctuidae)

Zhou

Yuan

et al. 2017

Genes

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Abstract:Insect kinins were shown to have diuretic activity, inhibit weight gain, and have antifeedant activity in insects. In order to study the potential of the TAT-fusion approach to deliver diuretic peptides per os to pest insects, the HezK I peptide from Helicoverpa zea, as a representative of the kinin family, was selected. The fusion gene TAT-HezK I was designed and was used to transform tobacco plants. As a means to further improve the stability of TAT-HezK I, a fusion protein incorporating HezK I, transactivator of transcription (TAT), and the cowpea trypsin inhibitor (CpTI) was also designed. Finally, the toxicity of the different tobacco transgenic strains toward Helicoverpa armigera was compared. The results demonstrated that TAT-HezK I had high toxicity against insects via transgenic expression of the peptide in planta and intake through larval feeding. The toxicity of the fusion TAT-HezK I and CpTI was higher than the CpTI single gene in transgenic tobacco, and the fusion TAT-HezK I and CpTI further enhanced the stability and bioavailability of agents in oral administration. Our research helps in targeting new genes for improving herbivore tolerance in transgenic plant breeding.

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Section: Methodsmentioning

confidence: 99%

Transgenic Tobacco Expressing the TAT-Helicokinin I-CpTI Fusion Protein Show Increased Resistance and Toxicity to Helicoverpa armigera (Lepidoptera: Noctuidae)

Zhou

Yuan

et al. 2017

Genes

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“…Tobacco leaf discs were transformed with A. tumefaciens strain LBA4404 by suspension in MS liquid medium solution. Leaves of about 20‐day‐old plantlet K326 clone at tissue culture stage were used as explants for transformation via the leaf‐disc method . In brief, infected leaf discs were grown in MS basal medium supplemented with 1.0 mg L −1 of 6‐benzyl aminopurine (6‐BA) and 0.1 mg L −1 of naphthaleneacetic acid (NAA) in the dark for 2 days at 25 °C, and then transferred on the same medium with 50 mg L −1 of kanamycin under a 16/8 h light/dark regime.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of TAT‐PTD–diapause hormone fusion protein in tobacco and its effect on the larval development of Helicoverpa armigera (Lepidoptera: Noctuidae)

Zhou

Yuan

et al. 2016

Pest Management Science

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“…Moreover, when two C-repeat-binding factor genes isolated from E. gunnii, EguCBF1a and -b, were constitutively expressed in a cold-sensitive Eucalyptus hybrid, it showed an improvement in freezing tolerance and development (Navarro et al 2011). Additionally, a significant increase in freezing tolerance in hybrid poplar (P. alba × Populus glandulosa [Uyeki]) was achieved on a small scale following over-expression of the Populus tomentosa (Carriere) ω-3 fatty-acid desaturase gene, FAD3 (Zhou et al 2010).…”

Section: Transgenes To Improve Tolerance To Cold Stressmentioning

confidence: 99%

Molecular and physiological responses to abiotic stress in forest trees and their relevance to tree improvement

2014

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Abiotic stresses, such as drought, salinity and cold, are the major environmental stresses that adversely affect tree growth and, thus, forest productivity, and play a major role in determining the geographic distribution of tree species. Tree responses and tolerance to abiotic stress are complex biological processes that are best analyzed at a systems level using genetic, genomic, metabolomic and phenomic approaches. This will expedite the dissection of stress-sensing and signaling networks to further support efficient genetic improvement programs. Enormous genetic diversity for stress tolerance exists within some forest-tree species, and due to advances in sequencing technologies the molecular genetic basis for this diversity has been rapidly unfolding in recent years. In addition, the use of emerging phenotyping technologies extends the suite of traits that can be measured and will provide us with a better understanding of stress tolerance. The elucidation of abiotic stress-tolerance mechanisms will allow for effective pyramiding of multiple tolerances in a single tree through genetic engineering. Here we review recent progress in the dissection of the molecular basis of abiotic stress tolerance in forest trees, with special emphasis on Populus, Pinus, Picea, Eucalyptus and Quercus spp. We also outline practices that will enable the deployment of trees engineered for abiotic stress tolerance to land owners. Finally, recommendations for future work are discussed.

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Improve freezing tolerance in transgenic poplar by overexpressing a ω-3 fatty acid desaturase gene

Cited by 12 publications

References 36 publications

Transgenic Tobacco Expressing the TAT-Helicokinin I-CpTI Fusion Protein Show Increased Resistance and Toxicity to Helicoverpa armigera (Lepidoptera: Noctuidae)

Transgenic Tobacco Expressing the TAT-Helicokinin I-CpTI Fusion Protein Show Increased Resistance and Toxicity to Helicoverpa armigera (Lepidoptera: Noctuidae)

Overexpression of TAT‐PTD–diapause hormone fusion protein in tobacco and its effect on the larval development of Helicoverpa armigera (Lepidoptera: Noctuidae)

Molecular and physiological responses to abiotic stress in forest trees and their relevance to tree improvement

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