Abstract:The analysis of explosives attracts recent research efforts due to homeland security needs and the broad demand for the clearance of minefields. While numerous studies have addressed the development of sensing platforms for nitroaromatic explosives, and specifically trinitrotoluene (TNT), the detection of more hazardous explosives, such as hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) or pentaerythritol tetranitrate (PETN), is less developed and needs further efforts, particularly the improvement of the sensit… Show more
“…Similar specific sensing of 1,3,5-trinitrotriazine, (29), RDX, was demonstrated with the Kemps acid, (30), -imprinted Au NPs composite [43]. Figure 6A shows the sensogram corresponding to the reflectance change of the imprinted Au NPs composite upon interaction with different concentrations of RDX.…”
Section: Molecularly Imprinted Au Nps Composites On Electrodes For Sementioning
confidence: 72%
“…Similarly, the imprint of molecular recognition sites for the 1,3,5-trinitrotriazine, RDX, (29), explosive has involved the use of Kemps acid (30) as an imprinting analog molecule [43]. RDX, (29), exists in a "chair-like" configuration, where the three nitro groups exist in quasiaxial positions.…”
Section: Molecularly Imprinted Au Nps Composites On Electrodes For Sementioning
confidence: 99%
“…Indeed, different biosensing platforms for analyzing antigen-antibody complexes [39], DNA hybridization [40], formation of aptamer-substrate complexes [22], and to follow biocatalytic processes [41], were developed. Figure 5A depicts the principles to electrosynthesize the imprinted Au NPs composites on Au electrodes and to use the resulting matrices for the SPR analysis of explosives [42,43]. Au NPs capped with electropolymeriza- (23) by the opening of the thiolated nucleic acid hairpin (22), associated with the electrode, and the formation of an aptamer-AMP complex, and the regeneration of the sensing electrode.…”
Section: Molecularly Imprinted Au Nps Composites On Electrodes For Sementioning
Metallic nanoparticles (NPs) of unique electronic and catalytic properties, or supramolecular nucleic acid-based nanostructures, provide new materials for electroanalytical applications. Metallic NPs are used for the electrical contacting of redox enzymes with electrodes and as electrocatalysts for the development of amperometric biosensors. DNA is used as a template for electroanalytical applications, such as the electrical contacting of enzymes with electrodes. Finally, an electrochemical method to synthesize molecularly imprinted Au NPs composites on surfaces is described, and the imprinted composites enable the selective and ultrasensitive detection of explosives, or their use as electrochemically triggered sponges for the uptake and release of substrates.
“…Similar specific sensing of 1,3,5-trinitrotriazine, (29), RDX, was demonstrated with the Kemps acid, (30), -imprinted Au NPs composite [43]. Figure 6A shows the sensogram corresponding to the reflectance change of the imprinted Au NPs composite upon interaction with different concentrations of RDX.…”
Section: Molecularly Imprinted Au Nps Composites On Electrodes For Sementioning
confidence: 72%
“…Similarly, the imprint of molecular recognition sites for the 1,3,5-trinitrotriazine, RDX, (29), explosive has involved the use of Kemps acid (30) as an imprinting analog molecule [43]. RDX, (29), exists in a "chair-like" configuration, where the three nitro groups exist in quasiaxial positions.…”
Section: Molecularly Imprinted Au Nps Composites On Electrodes For Sementioning
confidence: 99%
“…Indeed, different biosensing platforms for analyzing antigen-antibody complexes [39], DNA hybridization [40], formation of aptamer-substrate complexes [22], and to follow biocatalytic processes [41], were developed. Figure 5A depicts the principles to electrosynthesize the imprinted Au NPs composites on Au electrodes and to use the resulting matrices for the SPR analysis of explosives [42,43]. Au NPs capped with electropolymeriza- (23) by the opening of the thiolated nucleic acid hairpin (22), associated with the electrode, and the formation of an aptamer-AMP complex, and the regeneration of the sensing electrode.…”
Section: Molecularly Imprinted Au Nps Composites On Electrodes For Sementioning
Metallic nanoparticles (NPs) of unique electronic and catalytic properties, or supramolecular nucleic acid-based nanostructures, provide new materials for electroanalytical applications. Metallic NPs are used for the electrical contacting of redox enzymes with electrodes and as electrocatalysts for the development of amperometric biosensors. DNA is used as a template for electroanalytical applications, such as the electrical contacting of enzymes with electrodes. Finally, an electrochemical method to synthesize molecularly imprinted Au NPs composites on surfaces is described, and the imprinted composites enable the selective and ultrasensitive detection of explosives, or their use as electrochemically triggered sponges for the uptake and release of substrates.
“…SPR was introduced in the early 1990s as the underlying technology in affinity biosensors for biomolecular interaction analysis [5]. Now it has become a widely used analytical technique to monitor not only for DNA [6], protein binding and environmental contaminants [7,8], but also for small molecule micro-arrays [9].…”
“…To address this issues, surface imprinting techniques have been developed, where the imprinted cavities can be constructed only on the surface of MIPs, resulting in more effective removal of template molecules and improvement of accessibility of the imprinted cavities [33][34][35][36][37][38][39]. Although surface imprinting by surface-initiated polymerization has been reported [29,30] where the polymer thickness is significantly important and should be optimized, transcription-type molecular imprinting seems to be a cogent technique for the surface imprinting; the technique involves radical polymerization of pre-polymerization mixtures in between a target proteins-immobilized stamp and a substrate on which MIPs are to be prepared.…”
Abstract:Transcribed molecularly imprinted polymers (MIPs), prepared by using the biotinylated submicrometersized particles-immobilized stamp on which the biotinconjugated protein was hierarchically immobilized via avidin, were used successfully in the recognition of cytochrome c (Cyt) as a model protein. The transcribed MIP for Cyt was prepared on the gold-coated surface plasmon resonance (SPR) sensor chip, and the binding behavior of Cyt, myoglobin, ribonuclease A, lysozyme, and avidin was evaluated to confirm the selectivity for Cyt. The imprint effect of the transcribed MIP was revealed by comparing the MIP and the corresponding non-imprinted polymer prepared using the stamp without the Cyt immobilization.
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