Importation of Dengue Virus Type 3 to Japan from Tanzania and Côte d’Ivoire

Abstract

Travelers can introduce viruses from disease-endemic to non–disease-endemic areas. Serologic and virologic tests confirmed dengue virus infections in 3 travelers returning to Japan: 2 from Tanzania and 1 from Côte d’Ivoire. Phylogenetic analysis of the envelope gene showed that 2 genetically related virus isolates belonged to dengue virus type 3 genotype III.

Dengue virus isolation relying on antibody-dependent enhancement mechanism using FcγR-expressing BHK cells and a monoclonal antibody with infection-enhancing capacity

“…i Genetic characterization on the virus isolated from patient P28, a traveler returning from Tanzania, has been included in a prior study. 18 serotypes at various inoculation doses was determined. In addition, viral RNA was detected as early as day 2 post-infection with low inoculation dose (10 −1 PFU/dose) in the presence of mAb 4G2 in the culture supernatant fluids of Fc␥R-expressing BHK cells (Fig.…”

Detection of Dengue Virus Nonstructural Protein 1 (NS1) by Using ELISA as a Useful Laboratory Diagnostic Method for Dengue Virus Infection of International Travelers

“…15,16 Viral RNA was extracted using High Pure Viral RNA extraction kit (Roche Diagnostics, Mannheim, Germany) and DENV serotypes were determined by serotype-specific RT-PCR. 15,16 Anti-DENV IgM and IgG Antibody ELISA Dengue-virus specific IgM antibody in serum samples was determined using IgM capture ELISA (Dengue Fever Virus IgM Capture ELISA, Focus Diagnostics, CA, USA) according to the manufacturer's instructions. Dengue indirect IgG ELISA (Panbio, Queensland, Australia) was used for the detection of anti-DENV IgG antibody according to the manufacturer's instructions.…”

Chikungunya and Dengue Fever among Hospitalized Febrile Patients in Northern Tanzania

“…14 In East Africa, little is known about the prevalence of DENV infection and how the virus is maintained between epidemics. Although DENV outbreaks have not been reported in Tanzania, 13 DENV infection has been confirmed in travelers returning from the country, 15,16 suggesting that the virus is circulating in Tanzania. However, DENV infection, like CHIKV infection, is a diagnosis rarely considered by local clinicians, presumably due in part to a lack of information about disease prevalence.…”