Importance of Weak ABO Subgroups

Thakral, Beenu; Saluja, Karan; Bajpai, Meenu; Sharma, Ratti Ram; Marwaha, Neelam

doi:10.1309/x59t-aayp-epcn-bluj

Cited by 17 publications

(21 citation statements)

References 7 publications

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“…The phenotypic frequencies of these subgroups vary in different ethnic groups. The A 3 phenotype is the most common among all the weak subgroups with an estimated frequency of 1 in 1000 group A Danes [15], 1 in 16 667 in French donors [16], 1 in 90 000 Canadian donors [17], and 1 in 14 448 in north Indian blood donors [18]. The frequency of A 3 phenotype in our donors was comparable with that reported by Thakral et al in north Indian blood donors and Garraetta et al in French donors.…”

Section: Discussionsupporting

confidence: 88%

Blood group discrepancies at a tertiary care centre – analysis and resolution

Kaur

Basu

et al. 2013

Int J Lab Hematology

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Section: Discussionsupporting

confidence: 88%

Blood group discrepancies at a tertiary care centre – analysis and resolution

Kaur

Basu

et al. 2013

Int J Lab Hematology

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“…Thakral et al from India found overall incidence of weak subgroups as 1:5100 or 0.02% in their donor population [5]. Adsorption elution studies facilitate detection of weak antigens.…”

Section: Discussionmentioning

confidence: 99%

ABO blood group discrepancies among blood donors in Regional Blood Transfusion Centre GTB Hospital, Delhi, India

Sharma

Garg

Singh

2014

Transfusion and Apheresis Science

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“…13,21,22 The basic principle of this method is based on a strong specic antibody-antigen interaction, leading to the agglutination of RBCs inside the bre network of paper. However, its application for O Rh negative, a phenotype that has a signicantly high frequency in human population, 12 and weak ABO subgroups 12,23 have not been demonstrated. However, the plasma phase still moves forward, separating from agglutinated RBCs.…”

Section: Introductionmentioning

confidence: 99%

Low-cost blood plasma separation method using salt functionalized paper

Nilghaz

Shen

2015

RSC Adv.

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This study describes an extremely low-cost method for separating plasma in a sample of whole human blood on salt functionalized paper by means of osmotic pressure. When a sample of whole blood was introduced onto the salt functionalized paper, plasma dissolves the salt and places the red blood cells (RBCs) in a hypertonic medium. This leads to the generation of osmotic pressure across the cells membrane, and also the crenation of RBCs. The effect of different concentrations of salt on RBC deformation and crenation has been monitored using confocal microscopy. Depending upon the salt concentration, RBCs deform into various shapes under osmotic pressure. At high salt concentration, RBCs turn into deflated thin disks. This increases the RBCs' contact with one another and with fibres in the paper as well. Besides, the counter ion valency charge of the Na + suppresses the thickness of the charged double layer of RBC. Subsequently, aggregation of the deflated RBCs occurs. The aggregates are large enough to be separated chromatographically from the plasma phase of the wicking front.Our results show that 0.5 mL addition of 0.68 M (4% w/v) saline solution (NaCl) can provide sufficient plasma separation on a filter paper for diagnostic applications. A colorimetric blood glucose concentration assay is employed to demonstrate the efficiency of this plasma separation method on paper. The experimental investigation indicates that although the crenation of RBCs forced a small amount of water into plasma, this method is suitable for performing glucose assay in human blood on paper. Our method can enhance bioassays performed on microfluidic paper-based analytical devices (mPADs) by combining the separation and testing of plasma into a single device with no significant additional cost.

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Importance of Weak ABO Subgroups

Cited by 17 publications

References 7 publications

Blood group discrepancies at a tertiary care centre – analysis and resolution

Blood group discrepancies at a tertiary care centre – analysis and resolution

ABO blood group discrepancies among blood donors in Regional Blood Transfusion Centre GTB Hospital, Delhi, India

Low-cost blood plasma separation method using salt functionalized paper

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