Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification

Grotto, Denise; Maria, Lucas Santa; Valentini, Juliana; Paniz, Clóvis; Schmitt, Gabriela Cristina; Garcia, Solange Cristina; Pomblum, Valdeci Juarez; Rocha, João Batista Teixeira da; Farina, Marcelo

doi:10.1590/s0100-40422009000100032

Cited by 323 publications

(255 citation statements)

References 60 publications

(108 reference statements)

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“…MDA, an end product of polyunsaturated fatty acid oxygenation, is a reliable and commonly used marker of the overall lipid peroxidation level and the presence of oxidative stress (Moore and Roberts 1998;Grotto et al 2009). In the present study, the MDA concentrations of FCoV-seropositive cats were found to be significantly higher than cats seronegative for the feline coronavirus.…”

Section: Discussionmentioning

confidence: 99%

Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirus

Kayar¹,

Dokuzeylül²,

Kandemır³

et al. 2015

Vet. Med. - Czech

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Feline coronavirus (FCoV) is a highly contagious virus that is ubiquitous in multicat environments and may induce oxidative stress. This virus commonly causes an asymptomatic infection, which can persist in certain individuals. Sporadically and unpredictably, FCoV infection leads to feline infectious peritonitis (FIP), a highly fatal systemic immune-mediated disease. There are no data in the veterinary literature relating to oxidative stress in FCoV. Antioxidant capacity (TAC) can be attributed to single components in the defence systems against free radicals. The measurement of the total oxidant status (TOS) accurately reflects the oxidative status of blood plasma or serum. Nitric oxide (NO) acts as a free radical and contributes to host defences against oxidation. Malondialdehyde (MDA) is a reliable and commonly used marker of overall lipid peroxidation levels and the presence of oxidative stress. This study aimed to determine levels of oxidative stress markers, serum TAC, total oxidant capacity (TOC), NO and serum MDA in 24 cats seropositive for FCoVs and 15 cats seronegative for FCoVs. Significantly higher serum TOC, NO and MDA levels were found in seropositive animals (P < 0.001, P < 0.05 and P < 0.001, respectively) than in seronegative animals. In contrast, serum TAC levels were found to be significantly lower in seropositive cats compared with seronegative cats (P < 0.001). The results of the present study suggest that FCoV seropositivity is associated with oxidative stress and decreased antioxidant status.

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Section: Discussionmentioning

confidence: 99%

Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirus

Kayar¹,

Dokuzeylül²,

Kandemır³

et al. 2015

Vet. Med. - Czech

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“…AST, ALT and ALP levels of benzene treated group were increased oxidized species and reactive oxygen indicating the increased risk of cell membrane damage [37]. MDA is one of the most known secondary products of lipid peroxidation, and it can be used as a marker of cell membrane injury [38].…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to MDA, GSH levels profoundly decreased in kidney and liver tissues. Benzene and its metabolites are known to produce oxidized species indicating the increased risk of cell membrane damage [37]. The decrease in GSH level and increase in MDA level were associated with production of radicals and increased lipid peroxidation.…”

Section: Discussionmentioning

confidence: 99%

The effects of grape seed extract against toxicity of benzene on liver and kidney tissues of albino mice: biochemical evaluation

et al. 2015

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Objective: The objective of the present study was to investigate the possible effects of grape seed extract (GSE) against benzene-induced toxicity in Swiss albino mice. Methods: The animals were randomly divided into six groups each containing six mice. Group I, treated with distilled water; Group II and III orally treated with 50 mg/kg and 150 mg/kg body weight GSE, respectively. Group IV, orally treated with 250 mg/kg body weight benzene by using feeding cannula; Group V, orally treated with 50 mg/kg body weight GSE + 250 mg/kg body weight benzene; Group VI, orally treated with 150 mg/kg body weight GSE + 250 mg/kg of body weight benzene for 50 consecutive days. At the end of experimental period all mice were sacrificed; blood, liver and kidney tissues were removed after post-mortem examination. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine levels were analyzed from serum. Malondialdehyde (MDA) and reduced glutathione (GSH) levels were analyzed from isolated tissues. And also histopathological examinations of liver and kidney tissues were investigated. Results: Serum AST, ALT, ALP, BUN and creatinine levels were slightly increased in Group IV compared with the other tested groups (p<.05). Benzene-induced toxicity caused a significant decrease in GSH levels and a significant rise in MDA levels of liver and kidney tissues. Oral treatment with GSE significantly ameliorated the indices of hepatotoxicity, nephrotoxicity and lipid peroxidation induced by benzene. Both doses of GSE provided significant protection and the strongest effects were observed at the dose level of 150 mg/kg. Conclusion: Consequently, it was found that GSE has a significant positive effect in benzeneinduced toxicity, and its GSE effect is dose dependent. Key Words: Benzene, GSH, MDA grape seed Conflict of Interest: The authors have no conflict of interest. ÖZETAmaç: Bu çalışmanın amacı albino farelerde benzen tarafından oluşturulan toksisitesiye karşı üzüm çekirdeği ekstraktının muhtemel etkisini araştırmaktır. Metod: Her bir grupta 6 fare olmak üzere uygulama grupları oluşturulmuştur. Grup I, oral yolla distile su ile; Grup II, oral yolla 50 mg/kg GSE; Grup III, 150 mg/kg GSE; Grup IV, oral yolla kanula kullanılarak 250 mg/kg benzen; Grup V, 50 mg/kg GSE + 250 mg/kg benzen; Grup VI, 150 mg/kg GSE + 250 mg/kg benzen ile 50 gün süre ile beslenmişlerdir. Deney sürelerinin sonunda otopsi yoluyla farelerin kan, karaciğer ve böbrek dokuları alınmıştır. Aspartat aminotransferaz (AST), Alanin aminotransferaz (ALT), Alkalen fosfataz (ALP), kan üre azotu (BUN), kreatinin düzeyleri kan dokudan, malondialdehit (MDA), redükte glutatyon (GSH) ise karaciğer ve böbrek dokularında incelenmiştir. Ayrıca karaciğer ve böbrek dokularında histopatolojik değişimler de araştırılmıştır. Bulgular: Benzen uygulanan farelerde serum AST, ALT, ALP BUN ve kreatinin seviyelerinde kontrol grubuna kıyasla düşük düzeyde artış belirlenmiştir. Benzen tarafından oluşturulan oksidatif to...

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“…3) Escalating level of ROS can induce lipid peroxidation. 25) Further to support the measurement of ROS, we measured urine MDA, one of the most commonly used markers of overall lipid peroxidation level and oxidative stress. In our study, HW group showed a lower level of MDA than the PW group but there was no significant difference.…”

Section: Discussionmentioning

confidence: 99%

Positive Effects of Hydrogen Water on 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Yoon

Sajo

Ignacio

et al. 2014

Biological & Pharmaceutical Bulletin

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Atopic dermatitis (AD) is a chronically relapsing, pruritic, eczematous skin disorder accompanying allergic inflammation. AD is triggered by oxidative stress and immune imbalance. In the present study, we investigated the effect of drinking hydrogen water (HW) on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis in NC/Nga mice and found that HW ameliorated DNCB-induced AD-like clinical symptoms. In line with this, the level of reactive oxygen species in the HW group was significantly inhibited compared with that in the purified water (PW) group. In parallel, HW enhanced glutathione peroxidase activity in DNCBinduced AD as compared with the PW group. Accordingly, the levels of thymus and activation-regulated chemokine and cytokines were significantly decreased in the HW group compared with the PW group. Notably, the levels of Th 2 cytokine, interleukin-5 (IL-5), and proinflammatory cytokines such as tumor necrosis factor-α and IL-6 in HW-fed mice were significantly lower than in control and PW-fed mice. The total serum immunoglobulin E level was also markedly reduced in the HW group. The collective results indicate that HW suppresses DNCB-induced AD in NC/Nga mice via redox balance and immune modulation and could be a safe clinical fluid treatment for AD.Key words hydrogen water; atopic dermatitis; oxidative stress; immune modulation Atopic dermatitis (AD) is a chronically relapsing, pruritic, eczematous skin disorder accompanying allergic inflammation.1,2) AD is characterized by an impairment of the skinbarrier function, increased oxidative stress, and dysfunctional immune system. Intense infiltration of inflammatory cells release bioactive substance such as cytokines, chemokines, and reactive oxygen species (ROS).3) Of these AD's etiologic factors, inflammatory cytokines are known to regulate skin barrier, thereby aggravating the eczematous reaction in AD. 4)Most acute skin lesions in AD are exhibited by Th 2 inflammatory cytokines, whereas the chronic phase is characterized by Th 1 immune responses.5) The imbalance of cytokine network would play a critical role in the development of AD. 6,7)Together, oxidative stress such as increased ROS and lipid peroxidation is evident in any stage of AD. [8][9][10] An imbalance between ROS and antioxidants can lead to an elevated oxidative stress level. 11)Hydrogen molecule (H 2 ) has been widely used in medical applications as a safe and effective antioxidant and immunomodulator with minimal side effects.12-15) Unlike other antioxidants, which are unable to target organelles, H 2 can penetrate biomembranes and diffuse into the cytosol, mitochondria and nucleus. 13) Moreover, H 2 has been reported to have •OHscavenging activity in culture medium.14) Oral administration of hydrogen water (HW), inhalation of hydrogen gas, and injecting H 2 -dissolved saline is widely accepted to incorporate H 2 in the body. Recently, we reported that HW is effective in house mite-induced dermatitis via immunomodulation. 15) However, this study has a limitation owing to the lack of th...

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Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification

Cited by 323 publications

References 60 publications

Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirus

Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirus

The effects of grape seed extract against toxicity of benzene on liver and kidney tissues of albino mice: biochemical evaluation

Positive Effects of Hydrogen Water on 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

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