Importance of semen preparation in avoidance of reduced in vitro fertilization results attributable to bacteria

Forman, Robert G.; Guillett-Rosso, Frédérique; Fari, A; Volante, Monique; Frydman, R; Testart, J

doi:10.1016/s0015-0282(16)59070-0

Cited by 17 publications

(7 citation statements)

References 4 publications

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“…In contrast to the findings of Wong et al. (1986) and Forman et al. (1987), our study demonstrated a wide range of seminal microorganisms detected in semen samples of patients qualified to IVF procedure.…”

Section: Discussioncontrasting

confidence: 99%

“…Based on the literature data it may be assumed that systemic antibiotic treatment, together with selection of sperm on a special gradient and a lack of visible infection may exclude to the large extent the presence of pathogens during in vitro incubation for IVF (Huyser et al, 1991;Cottell et al, 1997;Michelmann, 1998;Nicholson et al, 2000). In contrast to the findings of Wong et al (1986) and Forman et al (1987), our study demonstrated a wide range of seminal microorganisms detected in semen samples of patients qualified to IVF procedure. This could be the result of specific population sample of infertile patients selected to the studies as well as of methodological approach.…”

Section: Group Of Pathogens Speciescontrasting

confidence: 42%

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Consequences of semen inflammation and lipid peroxidation on fertilization capacity of spermatozoa in in vitro conditions

Jȩdrzejczak¹,

Frączek

Szumała-Kąkol

et al. 2005

Int J of Andrology

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A body of data exists on reactive oxygen species (ROS) release, however, no direct correlation was found between the oxidative stress and infertility. The aim of the study was to measure semen oxidative stress and its correlation with classical in vitro fertilization (IVF) rate. A prospective study in academic non-profit institution where 79 infertile couples were subjected to IVF programme was conducted. Two infertile groups were discriminated according to the pronuclei presence in IVF. The main outcome measure (pronuclei presence) was then correlated with lipid peroxidation product in semen (ROS effect). Although the average IL-8 levels and malondialdehyde (MDA) content in semen did not differ between the studied subgroups (successful vs. non-successful fertilization), a statistically significant negative correlation was found between MDA level and fertilization rate in performed regression analysis. Thus we may suggest that MDA levels in seminal plasma may have prognostic value for IVF success.

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Section: Discussioncontrasting

confidence: 99%

Section: Group Of Pathogens Speciescontrasting

confidence: 42%

Consequences of semen inflammation and lipid peroxidation on fertilization capacity of spermatozoa in in vitro conditions

Jȩdrzejczak¹,

Frączek

Szumała-Kąkol

et al. 2005

Int J of Andrology

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“…The opposite result was obtained by Guillet-Rosso et al (1987). They checked 382 IVF couples and found out that although the fertilization rate and the early embryonic development were not negatively influenced by bacteria, the pregnancy rate per cycle was significantly reduced if the ejaculate contained bacteria.…”

Section: Bacteria and Ivfmentioning

confidence: 99%

“…Several studies have shown that previous infection with C. trachomatis influenced neither the spermiogram (Eggert-Kruse et al, 1990) nor the IVF outcome (Torode et al, 1987, Driscoll et al, 1991, Lessing et al, 1991. Forman et al (1987) took samples at five different stages during an IVF procedure and analysed them for bacteriological contaminations: (i) ejaculate; (ii) semen pellet after washing and centrifugation; (iii) swim-up suspension; (iv) insemination medium after the first 24 h of embryo culture; (v) culture medium after another 24 h of culture. Thus, bacteriological analyses after swim-up (Wong et al, 1986) and Percoll gradient centrifugation (Mathieu et al, 1988) had negative results.…”

Section: Bacteria and Ivfmentioning

confidence: 99%

“…Thus, bacteriological analyses after swim-up (Wong et al, 1986) and Percoll gradient centrifugation (Mathieu et al, 1988) had negative results. Forman et al (1987) took samples at five different stages during an IVF procedure and analysed them for bacteriological contaminations: (i) ejaculate; (ii) semen pellet after washing and centrifugation; (iii) swim-up suspension; (iv) insemination medium after the first 24 h of embryo culture; (v) culture medium after another 24 h of culture.…”

Section: Bacteria and Ivfmentioning

confidence: 99%

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Influence of bacteria and leukocytes on the outcome of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI)

Michelmann

2009

Andrologia

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Summary. The influence of bacteria and/or leukocytes on the outcome of IVF or ICSI is influenced by three factors which have little in common with in vivo conditions: 1) The process of ejaculate preparation (swim‐up, Percoll) with antibiotic buffered media; 2) The small amount of inseminated spermatozoa (100000 per culture); 3) The short cultivation time. From the very beginning, these factors limit whatever the influence of bacteria and leukocytes on fertilization and embryonic development in vivo may be. Despite the contradictory results published so far, the influence of bacteria and/or leukocytes on the functional integrity of spermatozoa during the process of IVF or ICSI can be ignored. Furthermore, during IVF or ICSI the spermatozoon does not act as a vector for the transportation of bacteria into the ooplasm.

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Microbial flora in semen during in vitro fertilization

Huyser

Fourie

Oosthuizen

et al. 1991

J Assist Reprod Genet

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Semen samples from 183 consecutive unselected men participating in an in vitro fertilization program were retrospectively studied to determine the bacterial and fungal contamination rate before and after antibiotic treatment. To ascertain the influence of semen preparation (wash and swim-up method) on the incidence of microorganisms, semen from 102 male patients was studied before and after swimup. Antimicrobial treatment by prescription of antibiotics decreased the incidence of pathogens by 16.3% (P less than 0.0001). Semen processing was more effective by ridding 57.4% of semen samples of microbial contaminants (P less than 0.0001). When infection of culture media was observed during routine microscopy, all infected oocytes were degenerated, without evidence of fertilization or pronuclei.

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Importance of semen preparation in avoidance of reduced in vitro fertilization results attributable to bacteria

Cited by 17 publications

References 4 publications

Consequences of semen inflammation and lipid peroxidation on fertilization capacity of spermatozoa in in vitro conditions

Consequences of semen inflammation and lipid peroxidation on fertilization capacity of spermatozoa in in vitro conditions

Influence of bacteria and leukocytes on the outcome of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI)

Microbial flora in semen during in vitro fertilization

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