Importance of miR-125a-5p and miR-122-5p expression in patients with HBV infection

Abstract

MicroRNAs (miRNAs) as a small RNA and post-transcriptional modulator are shown to have regulatory effects for different cellular activities and pathways, such as metabolism, virus replication and also cell growth. In addition, miRNAs can regulate the replication of the hepatitis B virus (HBV). Therefore, the expression profile of miRNAs was evaluated in HBV infected patient groups and healthy controls. The expression levels of following microRNAs (as noninvasive biomarkers) were compared in two experimental (those with various stages of HBV infection) and control groups to evaluate their diagnosis ability: miR-122-5p, miR-125a-5p, miR-199a-3p, miR-210-5p, miR-205-5p, miR-155-5p, miR-372-5p, and miR-1-5p. RNA extraction was performed for 45 serum samples. The miRCURY LNA™ Universal RT-miRNA-PCR system and miRNA PCR panels were used for measuring microRNA expression profiles. To normalize quantitative values, the endogenous reference by UniSp6 expression was used.Serum miR-125a-5p and miR-122-5p were significantly higher in patients in different stages of HBV infection (p<0.001) than in controls (p<0.001). Receiver operating characteristic (ROC) curve analyses suggested that serum miR-125a-5p and miR-122-5p have significant diagnostic value for HBV infection. A significant difference was not found in terms of serum levels of other miRs (miR-199a-3p, miR-210-5p, miR-205-5p, miR-155-5p, miR-372-5p and miR-1-5p). Results suggest that miR-125a-5p and miR-122-5p can be used as possible noninvasive biomarkers for monitoring of HBV infection need to confirm in future completed studies.

Importance of miR-141-5p and miR-501-5P expression in patients with HBV infection

“…The result of the survey of miRNA 141-5p expression in patients HBV like miR-NA 501-5p It is not diagnostic value (27). In the study like we found no significant difference between the six miRs (miR-199a-3p, miR-210, miR-205, miR155, miR-372, and miR-1) in the serum of patients infected with HBV compared to the control group (28). Our data indicated that mean ALT and AST were significantly higher (p<0.001) in the EPCH group than in ENCH and OHB and healthy controls, suggesting a positive correlation with hepatic necroinflammation.…”

Recent Advances in Biosensors for Detection of COVID-19 and Other Viruses

“…As a result of the evolution of receptors for biological compounds over millions of years, scientists are currently designing hybrid devices using nanoscience and biology, known as Nano-biosensors so that they can detect molecules in extremely small concentrations, inaccessible regions, and even within cells [26] . Small RNAs (miRNAs) have been demonstrated to have regulatory effects on a variety of cellular processes and pathways, including metabolism, viral replication, and cell development [27] . Droplets of varying sizes are the primary means by which the COVID-19 infection spreads.…”

ATOH8 promotes HBV immune tolerance by inhibiting the pyroptotic pathway in hepatocytes

“…The limited efficacy is primarily associated with the ability of HBV to evade innate immunity through various mechanisms (3)(4)(5)(6), including destroying the corresponding recognition receptors (7) and depleting the inflammatory factors important for the activation of adaptive immunity (8). Studies on HBV are becoming increasingly diverse, ranging from studies at the molecular and protein level, to studying the impact of the occurrence and development of viral hepatitis (9)(10)(11). However, to the best of our knowledge, only one study (12) has investigated the effects of transcription factors on the invasive activity of HBV in hepatocytes.…”