Implications of visfatin genetic variants in the metabolic profile of the Romanian pediatric population

Abstract

Background: Conflictual results regarding the relationship between plasmatic level of visfatin and obesity could be explained by the influence of the gene variants involved in the synthesis or action of these hormones.Objectives: The present study examined the potential implication of single nucleotide polymorphisms (SNPs) of nicotinamide phosphoribosyltransferase (NAMPT) gene that encodes visfatin, in obesity, in a Romanian pediatric population.Method: A case-control study was conducted on a group of 213 children, divided into two: the case group - 130 overweight and obese children with BMI >1 SD, and the control group - 83 children with normal BMI. The variables analyzed were age, sex, anthropometric parameters, body composition based on bioimpedance analysis, lipid profile, visfatin and insulin plasmatic levels, rs4730153 and rs2302559 visfatin SNPs.Results: Significant associations were not found between rs4730153 and rs2302559 visfatin SNPs and obesity. Regarding lipid metabolism, there are statistically significant differences between triglyceride levels according to NAMPT rs2302559 genotypes (p=0.045), with heterozygous genotype having the highest level of triglycerides, and also between cholesterol levels according to NAMPT rs4730153 genotypes (p=0.030), with carriers of heterozygote genotype having the highest level of cholesterol. There is a statistically significant difference between the studied parameters in the investigated groups, regarding cholesterol, in carrier of wild-type genotype of NAMPT rs2302559 (p=0.040) and carrier of wild-type genotype of NAMPT rs4730153 (p=0.036). We observed no association of NAMPT rs4730153 and rs2302559 with visfatin levels in the studied groups. Visfatin level was lower in the case group and was correlated with weight (p=0.042), abdominal circumference (p=0.010), waist to height ratio (p=0.040), but not with the elements of the metabolic profile.Conclusion: NAMPT rs2302559 and rs4730153 polymorphisms do not seem to have a major impact in the development of obesity in children, however there may be an association with lipid profile, but further studies are needed..

Association of Visfatin gene polymorphism with obesity related metabolic disorders among Pakistani population; a case control study

“…Previous ndings revealed no signi cant differences between the genotypes of rs2302559 and anthropometric parameters (BMI, waist circumference, waist to height ratio, and fat mass) (Chen et al, 2007). Visfatin levels are not signi cantly different between rs2302559 genotypes in earlier study, but a study that has looked at 243 obese children found that rs2302559 variant allele carriers has lower fasting serum visfatin and lower fasting plasma glucose than wild-type allele carriers (Vasilache et al, 2020). However according to our ndings, individuals with any genetic variation of visfatin i.e., rs2302559 and rs1215113036 has more susceptible to metabolic syndrome.…”

<p>Plasma RBP4 Level in Association with Body Composition, Metabolic Profile, STRA6 and RBP4 Gene Polymorphisms in Obese Romanian Children</p>

“…This disorder is caused by the interaction between genetic and environmental factors. 4,32,33 Abdominal obesity by itself is an independent risk factor for insulin resistance and the development of type 2 diabetes mellitus. 34 Our study found significant higher levels of both insulin and HOMA IR in the obese group, results concordant to the study of 173 obese and multi-ethnic adolescents which concluded that gender, pubertal stage, BMI, WC, and body fat percentage had significant, positive correlations with fasting insulin and HOMA IR.…”

“…This disorder is caused by the interaction between genetic and environmental factors. 4 , 32 , 33 Abdominal obesity by itself is an independent risk factor for insulin resistance and the development of type 2 diabetes mellitus. 34 …”

Evaluation of Plasma AA/DHA+EPA Ratio in Obese Romanian Children

“…Clinical evaluation was performed by an experimented endocrinologist using standardized protocols for obesity assessment: height (cm), weight (kg), body mass index (BMI) (kg/m 2 ) expressed as raw values and standard deviation (SD) score, waist circumference (WC), waist-toheight ratio (WHtR) as a marker of central adiposity, tricipital skinfold thickness (TST), and body fat composition using bio-impedance analysis (total fat mass, percentage of fat mass, freefat mass) as previously described (20). For lipids, glucose and endocrine profile (Insulin, Visfatin, RBP-4) the blood was withdrawn after at least 8 hours fasting, during the morning, in EDTA collection tubes.…”

“…Since the differences between obese and nonobese groups regarding the demographic and anthropometric parameters were analyzed elsewhere (20), in this subsequent study we performed the analysis of the basal FAs plasma levels in the above mentioned groups (Table 2).…”