Implications of the use of serological and molecular methods to detect infection by Leishmania spp. in urban pet dogs

Paz, Gustavo Fontes; Rugani, Jeronimo Marteleto Nunes; Marcelino, Andreza Pain; Gontijo, Célia Maria Ferreira

doi:10.1016/j.actatropica.2018.03.018

Cited by 25 publications

(16 citation statements)

References 18 publications

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“…Molecular diagnostic tests are not routinely used in public health programs because of the relative difficulties regarding the need for complex laboratorial infrastructure, the elevated costs, and the lack of commercially available assays, as well as other limitations. 7 , 8 , 9 , 10 …”

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confidence: 99%

“…The program sequentially uses two serological tests, which present a lower diagnostic accuracy in asymptomatic dogs or in those in the initial stages of the infection. (3,4,5,6,7,8) Although some works indicate that serological tests may be used with efficacy for diagnosing asymptomatic animals, (6,9) the performance of serological tests may be up to 20% inferior to the performance of molecular tests in that subgroup. (7,8,9,10) doi: 10.1590/0074-02760180452 Financial support: FAPDF (grant no.…”

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confidence: 99%

“…(3,4,5,6,7,8) Although some works indicate that serological tests may be used with efficacy for diagnosing asymptomatic animals, (6,9) the performance of serological tests may be up to 20% inferior to the performance of molecular tests in that subgroup. (7,8,9,10) doi: 10.1590/0074-02760180452 Financial support: FAPDF (grant no. 193.000.867/2015 and the public call 06/2013 grant no.…”

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confidence: 99%

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Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests

Teixeira

Silva

Vital

et al. 2019

Mem. Inst. Oswaldo Cruz

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BACKGROUND Studies aimed at validating canine visceral leishmaniasis diagnostic tests present heterogeneous results regarding test accuracy, partly due to divergences in reference standards used and different infection evolution periods in animals. OBJECTIVE This study aimed to evaluate the accuracy of the rapid test-dual path platform (TR-DPP) (Biomanguinhos®), EIE-Leishmaniose-Visceral-Canina-Biomanguinhos (EIE-LVC) (Biomanguinhos®), enzyme-linked immunosorbent assay (ELISA) rK39 (in-house), and the direct agglutination test (DAT- Canis ) against a reference standard comprising parasitological and molecular techniques. METHODS A phase II/III validation study was carried out in sample sera from 123 predominantly asymptomatic dogs living in an area endemic for visceral leishmaniasis. FINDINGS Sixty-nine (56.1%) animals were considered infected according to the reference standard. For each test, the sensitivity and specificity, respectively, were as follows: TR-DPP, 21.74% [confidence interval (CI)95% 13.64% to 32.82%] and 92.59% (CI95% 82.45% to 97.08%); EIE-LVC, 11.59% (CI95% 5.9% to 21.25%) and 90.74% (CI95% 80.09% to 95.98%); ELISA rK39, 37.68% (CI95% 27.18% to 49.48%) and 83.33% (CI95% 71.26% to 90.98%); and DAT- Canis , 18.84% (CI95% 11.35% to 29.61%) and 96.30% (CI95% 87.46% to 98.98%). CONCLUSION We concluded that improving the sensitivity of serum testing for diagnosing asymptomatic dogs must constitute a priority in the process of developing new diagnostic tests to be used in the visceral leishmaniasis control program in Brazil.

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Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests

Teixeira

Silva

Vital

et al. 2019

Mem. Inst. Oswaldo Cruz

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“…Since then, public laboratories use the ICT Dual Path Platform rK28 (DPP CVL, Bio-Manguinhos, Rio de Janeiro, Brazil), followed by ELISA with soluble Leishmania major antigen (Bio-Manguinhos). Despite the improvement of the diagnosis, debate on the accuracy of this protocol persists 5,6 . Moreover, the limited supply of diagnostic kits for ELISA in Brazil represents a problem, mainly for private laboratories.…”

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confidence: 99%

Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil

et al. 2021

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Dogs are the main reservoirs in the domestic transmission cycle of visceral leishmaniasis, and the diagnosis is essential for the effectiveness of the control measures recommended by the Brazilian Ministry of Health. We assessed the diagnostic performance of the ELISA-Vetlisa/BIOCLIN prototype with serum samples from 200 dogs, in triplicate, including symptomatic, oligosymptomatic, asymptomatic, and healthy dogs, originated by two distinct panels (A and B) characterized by parasitological tests as the reference standard. In this study, the prototype kit showed a 99% sensitivity (95%CI: 94.5-100.0) and a 100% specificity (95%CI: 96.4-100.0). The sensitivity of the prototype kit did not vary significantly with the clinical status of the dogs. Considering the final result classification (positive or negative), agreement between the results of repeated tests was almost perfect (kappa = 0.99; 95%CI: 0.98-1.00). ELISA-Vetlisa/BIOCLIN is a promising option for the serological diagnosis of canine visceral leishmaniasis in Brazil.

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“…It is considered a serious public health problem since L. infantum is the causative agent of human visceral leishmaniasis, the more severe form of this disease. However, other species have also been reported in dogs, such as L. amazonensis, L. mexicana, and L. braziliensis (Gontijo and De Carvalho, 2003;Castro et al, 2007;Valdivia et al, 2017;Paz et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

DH82 Canine and RAW264.7 Murine Macrophage Cell Lines Display Distinct Activation Profiles Upon Interaction With Leishmania infantum and Leishmania amazonensis

Nadaes

Costa

Santana

et al. 2020

Front. Cell. Infect. Microbiol.

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Implications of the use of serological and molecular methods to detect infection by Leishmania spp. in urban pet dogs

Cited by 25 publications

References 18 publications

Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests

Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests

Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil

DH82 Canine and RAW264.7 Murine Macrophage Cell Lines Display Distinct Activation Profiles Upon Interaction With Leishmania infantum and Leishmania amazonensis

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