Implication of cell wall constituents in the sensitivity of Kluyveromyces lactis strains to amphotericin B

Ramanandraibe, Elise; Younsi, Mohamed; Coulon, Joël; Loppinet, V.; Hakkou, Abdelkader; Bonaly, R.

doi:10.1016/s0923-2508(98)80026-0

Cited by 18 publications

(14 citation statements)

References 15 publications

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“…Gale et al(17) also reported that, as a result of changes in the cell wall, stationary phase cultures of C.albicans acquired phenotypic resistance to AmB two to three orders of magnitude greater than the cells in the exponential phase of growth. Recently, Ramanandraibe et al (38) …”

Section: Discussionmentioning

confidence: 99%

Alteration of Cell Wall Composition Leads to Amphotericin B Resistance in Aspergillus flavus

Seo

Akiyoshi

Ohnishi

1999

Microbiology and Immunology

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An amphotericin B (AmB)‐resistant mutant was isolated from a wild‐type AmB‐susceptible strain of Aspergillus flavus by serial transfer of conidia on agar plates containing stepwise increased concentrations of AmB up to 100 μtg ml−1. The acquired resistance of my celia was specific for polyene‐antibiotics AmB, nystatin and trichomycin. Spheroplasts derived from the resistant mycelia were as susceptible to AmB as the wild‐type. Chemical analysis of the cell wall revealed that levels of alkali‐soluble and ‐insoluble glucans were significantly higher in the resistant mycelia as compared to those in the wild‐type. When resistant mycelia were treated with SDS, they adsorbed as much AmB as wild‐type mycelia. These results suggest that alterations in the cell wall components of mycelia, especially 1,3‐α‐glucan and protein complex in the outermost wall layer, lead to AmB resistance in A. flavus.

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Section: Discussionmentioning

confidence: 99%

Alteration of Cell Wall Composition Leads to Amphotericin B Resistance in Aspergillus flavus

Seo

Akiyoshi

Ohnishi

1999

Microbiology and Immunology

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“…Although no evidence at present indicates that the phosphatase functions with LmrA in MDR, it is convergently transcribed with the LmrA gene, a fact highly suggestive of a role in MDR. In addition to the evidence from bacteria, results from yeast suggest that ectophosphatase activity increases resistance to the polygenic antifungal agent, amphotericin B (Ramanandraibe et al, 1998). To date, however, no studies have functionally linked ecto-ATPase activity and xenobiotic resistance.…”

Section: Atp Gradientsmentioning

confidence: 99%

A Role for Ectophosphatase in Xenobiotic Resistance

et al. 2000

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Xenobiotic resistance in animals, plants, yeast, and bacteria is known to involve ATP binding cassette transporters that efflux invading toxins. We present data from yeast and a higher plant indicating that xenobiotic resistance also involves extracellular ATP degradation. Transgenic upregulation of ecto-ATPase alone confers resistance to organisms that have had no previous exposure to toxins. Similarly, cells that are deficient in extracellular ATPase activity are more sensitive to xenobiotics. On the basis of these and other supporting data, we hypothesize that the hydrolysis of extracellular ATP by phosphatases and ATPases may be necessary for the resistance conferred by P-glycoprotein.

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“…were also involved in the sensitivity or resistance of the cells to AmB [11,12]. Studying the cell wall composition of two AmB resistant Kluyveromyces strains, results indicated that the chitin levels were 6^9-fold higher than that in the respective wildtype strains.…”

Section: Introductionmentioning

confidence: 99%

Change of cell wall chitin content in amphotericin B resistant Kluyveromyces strains

Bahmed

2002

FEMS Microbiology Letters

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The culture of two Kluyveromyces species, Kluyveromyces lactis (ATCC 96897) and Kluyveromyces bulgaricus (ATCC 96631), in the presence of subinhibitory doses of amphotericin B leads to the selection of mutants which are resistant to this polyene. The mutants show an alteration of their cell wall composition with the main change corresponding to an increase of chitin. The enzyme activities involved in the metabolism of this polymer, i.e. chitin synthases and chitinase, were measured. The results demonstrate that in both mutants the activity of chitinase was drastically decreased by 99% in comparison with the activity measured in the corresponding wild-type strain while no significant change of the chitin synthase I, II and III activities could be detected.

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Implication of cell wall constituents in the sensitivity of Kluyveromyces lactis strains to amphotericin B

Cited by 18 publications

References 15 publications

Alteration of Cell Wall Composition Leads to Amphotericin B Resistance in Aspergillus flavus

Alteration of Cell Wall Composition Leads to Amphotericin B Resistance in Aspergillus flavus

A Role for Ectophosphatase in Xenobiotic Resistance

Change of cell wall chitin content in amphotericin B resistant Kluyveromyces strains

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