This development aims to produce a product in the form of a HOTS-based assessment test on reaction rate. The development was done according to the ADDIE development model. However, due to the limited situation implementation and evaluation stages were not yet completed. The developed instrument consists of 15 items in the form of multiple-choice with 5 possible choices. The content of the assessment instrument was validated by three experts and empirical validation was done by subjecting the test to 29 students of the 3 rd semester majoring in Chemistry and 61 high school students majoring in science. Content validation showed the instrument was 93.33% valid which fell to very good criteria. Empirical validation showed that all 15 items were valid and reliable. Item validity had rcount value between 0.225 and 0.737 (rtable= 0.207) at 95% confidence level, while reliability test gave Cronbach's Alpha value equal to 0.805. In terms of item difficulty, 10 items were classified as moderate, 1 item was classified as easy, and 4 items were classified as difficult. As to the effectivity of distractor effect, 3 items out of 15 items contained ineffective distracting effect, which had been revised. Based on these results, it can be concluded that the assessment test was suitable for further stages, implementation and evaluation. However, the last two stages of ADDIE would be better to perform with the prerequisite condition when the students are learning in an environment that is constructed to developed HOTS.