Implementation of Cryopreservation for Garlic Genetic Resources by the Droplet Vitrification Procedure

Kim, Haeng-Hoon; Lee, Joung-Kwan; Ji, Jae-Jun; Nam, Sang-Sik; Hwang, Hae-Sung; Cho, Eun-Gi; Engelmann, Florent

doi:10.17660/actahortic.2007.760.27

Cited by 4 publications

(1 citation statement)

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“…Just after, Niwata [41] reported the conservation of A. sativum apical meristems using the same method. Over time, many studies tested cryopreservation procedures for Allium, including vitrification [42][43][44][45][46][47], encapsulation-dehydration [48,49], droplet vitrification [46,[50][51][52][53][54][55], and cry-oplate methods [23]. A variety of explants were examined in these different cryopreservation procedures, including shoot tips [23,42,[50][51][52][53][56][57][58], unripe inflorescences and bulbil primordia [54,55], pollen [59], and embryonic callus [47].…”

Section: Amaryllidaceaementioning

confidence: 99%

The Cryopreservation of Medicinal and Ornamental Geophytes: Application and Challenges

Merzougui

Benelli̇

Boullani

et al. 2023

Plants

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Nowadays, plant genetic resources are often at risk of loss and destruction. Geophytes are herbaceous or perennial species that are annually renewed by bulbs, rhizomes, tuberous roots, or tubers. They are often subject to overexploitation, which, combined with other biotic and abiotic stresses, can make these plants more vulnerable to a decline in their diffusion. As a result, multiple endeavors have been undertaken to establish better conservation strategies. Plant cryopreservation at ultra-low temperatures in liquid nitrogen (−196 °C) has proven to be an effective, long-term, low-cost, and suitable conservation method for many plant species. Over the last two decades, major advances in cryobiology studies have enabled successful explants of multiple genera and types, including pollen, shoot tips, dormant buds, and zygotic and somatic embryos. This review provides an update on recent advances and developments in cryopreservation and its application to medicinal and ornamental geophytes. In addition, the review includes a brief summary of factors limiting the success of bulbous germplasm conservation. The critical analysis underpinning this review will benefit biologists and cryobiologists in their further studies on the optimization of geophyte cryopreservation protocols and will support a more complete and wider application of knowledge in this area.

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