“…The homogenate was clarified by centrifugation at 16,000 g for 5 minutes, following which 20 μg protein samples were electophoresed through Bio-Rad Mini-Protean TGX 4–15% gels and transferred to PVDF membranes. Blots were probed with primary antibodies: (a) Oct 4 (anti-rabbit Oct 4, polyclonal, 1:500; BioVision, Inc., Milpitas, California, U.S.A.) [ 12 ]; (b) ASMA (anti-human ASMA, monoclonal, 1:1,000; Calbiochem, Billerica, Massachusetts, USA) [ 12 ]; (c) Calponin I (anti-mouse calponin 1, monoclonal, 1:500; Santa Cruz Biotechnology, Dallas, Texas, U.S.A) [ 12 ]; (d) VEGF (anti-mouse VEGF, monoclonal, 1:500; Santa Cruz Biotechnology) [ 43 , 44 ]; (e) Von Willebrand (anti-mouse Von Willebrand, polyclonal, 1:500; Abcam, Cambridge, Massachusetts, U.S.A.) [ 45 ]; (f) CD31 (anti-mouse CD31, monoclonal, 1:1000, Abcam) [ 46 ]; (g) BDNF (anti-mouse BDNF, rabbit monoclonal, 1:1000; Abcam) [ 38 ]; (h) MHC (fast) (anti-mouse MHC-II, monoclonal, 1:5000; Abcam) [ 12 ]; (i) myostatin (anti-mouse myostatin, rabbit polyclonal 1:1,000; Chemicon International Inc, Billerica, Massachusetts, U.S.A.) [ 12 , 47 ]; (j) follistatin (anti-mouse follistatin, rabbit polyclonal, 1:1000, Abcam) [ 48 ]; and (k) GAPDH (anti-mouse GAPDH, monoclonal, 1:5,000, Chemicon International).…”