Implanted Cell-Dense Prevascularized Tissues Develop Functional Vasculature That Supports Reoxygenation After Thrombosis

White, Sean; Pittman, Chelsea R.; Hingorani, Ryan; Arora, Rajan; Esipova, Tatiana V.; Vinogradov, Sergei A.; Hughes, Christopher C.W.; Choi, Bernard; George, Steven C.

doi:10.1089/ten.tea.2013.0311

Cited by 39 publications

(36 citation statements)

References 77 publications

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“…This finding suggests that implanted constructs attracted host blood vessels from more superficial (and vascular) regions of the mouse dermis. Any vascularization within a construct occurred at its surface, consistent with the findings of others [20].…”

Section: Thymus Mscs Promote In Vivo Vascularizationsupporting

confidence: 88%

Characterization and Angiogenic Potential of Human Neonatal and Infant Thymus Mesenchymal Stromal Cells

Wang

Mundada

Johnson

et al. 2015

Stem Cells Translational Medicine

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Mesenchymal stromal cells (MSCs) are present in large numbers in discarded neonatal and infant thymus tissue and can be isolated by an explant culture method. Explant culture‐isolated thymus MSCs demonstrate the classical characteristics of bone marrow MSCs. Thymus MSCs also possess proangiogenic qualities and deserve further consideration as a potential cell therapeutic agent to promote tissue regeneration and repair and for use in strategies to vascularize engineered tissues.

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Section: Thymus Mscs Promote In Vivo Vascularizationsupporting

confidence: 88%

Characterization and Angiogenic Potential of Human Neonatal and Infant Thymus Mesenchymal Stromal Cells

Wang

Mundada

Johnson

et al. 2015

Stem Cells Translational Medicine

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“…To characterize longitudinally the microcirculation of the dorsal window chamber, intravital microscopy is typically used [55–58]. This method provides invaluable information on the microvasculature, but its use is limited largely to study only a small subregion of the window chamber.…”

Section: Development Of New Treatment Approachesmentioning

confidence: 99%

The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities

Choi

Tan

Jia

et al. 2016

IEEE J. Select. Topics Quantum Electron.

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Here, we review our current knowledge on the etiology and treatment of port-wine stain (PWS) birthmarks. Current treatment options have significant limitations in terms of efficacy. With the combination of 1) a suitable preclinical microvascular model, 2) laser speckle imaging (LSI) to evaluate blood-flow dynamics, and 3) a longitudinal experimental design, rapid preclinical assessment of new phototherapies can be translated from the lab to the clinic. The combination of photodynamic therapy (PDT) and pulsed-dye laser (PDL) irradiation achieves a synergistic effect that reduces the required radiant exposures of the individual phototherapies to achieve persistent vascular shutdown. PDL combined with anti-angiogenic agents is a promising strategy to achieve persistent vascular shutdown by preventing reformation and reperfusion of photocoagulated blood vessels. Integration of LSI into the clinical workflow may lead to surgical image guidance that maximizes acute photocoagulation, is expected to improve PWS therapeutic outcome. Continued integration of noninvasive optical imaging technologies and biochemical analysis collectively are expected to lead to more robust treatment strategies.

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“…Morphological characterization has revealed that in vitro pre-vascularized tissues contained well-organized vascular structures and could accelerate vascularization time by providing adequate blood supply to the seeded cells. Unfortunately, host-implant anastomosis of in vitro pre-vascularized tissues usually occurs within several days after implantation;212223 thus, integration of reconstructed tissue with the host was inefficient. An in vivo pre-vascularization strategy has been developed to fabricate large-sized, vascularized implantable constructs.…”

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confidence: 99%

Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair

Kim

Atala

et al. 2016

Sci Rep

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Reconstructing functional volumetric tissue in vivo following implantation remains a critical challenge facing cell-based approaches. Several pre-vascularization approaches have been developed to increase cell viability following implantation. Structural and functional restoration was achieved in a preclinical rodent tissue defect; however, the approach used in this model fails to repair larger (>mm) defects as observed in a clinical setting. We propose an effective cell delivery system utilizing appropriate vascularization at the site of cell implantation that results in volumetric and functional tissue reconstruction. Our method of multiple cell injections in a progressive manner yielded improved cell survival and formed volumetric muscle tissues in an ectopic muscle site. In addition, this strategy supported the reconstruction of functional skeletal muscle tissue in a rodent volumetric muscle loss injury model. Results from our study suggest that our method may be used to repair volumetric tissue defects by overcoming diffusion limitations and facilitating adequate vascularization.

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Implanted Cell-Dense Prevascularized Tissues Develop Functional Vasculature That Supports Reoxygenation After Thrombosis

Cited by 39 publications

References 77 publications

Characterization and Angiogenic Potential of Human Neonatal and Infant Thymus Mesenchymal Stromal Cells

Characterization and Angiogenic Potential of Human Neonatal and Infant Thymus Mesenchymal Stromal Cells

The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities

Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair

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