Impedimetric paper-based biosensor for the detection of bacterial contamination in water

Rengaraj, Saravanan; Cruz-Izquierdo, Alvaro; Scott, Janet L.; Lorenzo, Mirella Di

doi:10.1016/j.snb.2018.03.020

Cited by 103 publications

(50 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, carbon-based conductive ink is printed onto paper surface [25,26]. In this context, Rengaraj et al [27] fabricated a paper-based electrode with the high-quality printing paper using only three layers of printing.…”

Section: Paper-based Analytical Devices 21 Substrate Materialsmentioning

confidence: 99%

“…Screen printing is another technique used to fabricate paper-based devices, particularly for electrochemical analysis ( Figure 1B). For example, Rengaraj et al [27] fabricated a paper-based electrode by printing three layers of a carbon-based conductive ink onto hydrophobic printer paper. Other fabrication techniques include a simple procedure of cutting by punching [18,19], obtaining discs with millimetric dimensions that can be functionalized and/or introduced into devices, such as commercial screen-printed electrodes [18].…”

Section: Fabrication Proceduresmentioning

confidence: 99%

“…The electrochemical paper-based biosensors are based on the modification of paperbased platforms placed in commercial screen-printed electrodes [17,18], or rely on the fabrication of lab-made functionalized paper-based screen-printed electrodes [27].…”

Section: Transducersmentioning

confidence: 99%

“…In another work, the recombinant human metallothionein 1a, a metal-binding protein [18], was used for the recognition of As 3+ and Hg 2+ in water. The tetrameric protein lectin concanavalin A (obtained from Canavalia ensiformis), selective to carbohydrates on bacterial cells, was selected as a biorecognition element of bacterial cultures from sewage sludge [27].…”

Section: Biorecognition Elementsmentioning

confidence: 99%

See 3 more Smart Citations

Paper-Based Biosensors for Analysis of Water

Peixoto¹,

Machado²,

Oliveira³

et al. 2019

Biosensors for Environmental Monitoring

View full text Add to dashboard Cite

The presence of contaminants in water generates a great concern worldwide. As contaminants, we can refer different classes of chemicals, such as pharmaceuticals, personal care products, heavy metals, and also microorganisms, such as waterborne pathogens. Some of the chemical compounds have the potential to bioaccumulate in the aquatic biota. Hence, the development of simple and portable methods for the detection of contaminants in the aquatic environment can improve their monitoring and, consequently, the study of their environmental impact. In this context, the development of paper-based analytical tools and also of biosensor devices has been exploited for quantitative and semiquantitative analysis of several contaminants in different water matrices. The association of these two analytical strategies can provide the implementation of low-cost, portable, and easily handled methods for detecting chemical and biological contaminations in water. In this chapter, we provide a review of the developed paper-based analytical biosensors, highlighting the features of the paper-based (paper substrate and fabrication procedures) and biosensor devices (transducers and biorecognition elements). Moreover, the application of the referred paper-based biosensors for the detection of different water contaminants (pathogens, pharmaceuticals, and heavy metals) in environmental and wastewater samples is discussed.

show abstract

Section: Paper-based Analytical Devices 21 Substrate Materialsmentioning

confidence: 99%

Section: Fabrication Proceduresmentioning

confidence: 99%

Section: Transducersmentioning

confidence: 99%

Section: Biorecognition Elementsmentioning

confidence: 99%

See 2 more Smart Citations

Paper-Based Biosensors for Analysis of Water

Peixoto¹,

Machado²,

Oliveira³

et al. 2019

Biosensors for Environmental Monitoring

View full text Add to dashboard Cite

show abstract

“…The immunosensor detection limit was 2.0 CFU/ml, and linear working range was 10-10 4 CFU/ml. Rengaraj et al fabricated an impedimetric paper-based biosensor for the detection of bacterial contamination in water [73]. They used lectin concanavalin A as a bioselective element due to its stability to interact with mono-and oligosaccharides on bacterial cells.…”

Section: Biosensors For Bacterial Analysismentioning

confidence: 99%

Challenges and Applications of Impedance-Based Biosensors in Water Analysis

Kivirand¹,

Min²,

Rinken³

2019

Biosensors for Environmental Monitoring

View full text Add to dashboard Cite

Monitoring of the environment is a global priority due to the close connection between the environmental pollution and human health. Many analytical techniques using various methods have been developed to detect and monitor the levels of pollutants (pesticides, toxins, bacteria, drug residues, etc.) in natural water bodies. The latest trend in modern analysis is to measure pollutants in real-time in the field. For this purpose, biosensors have been employed as cost-effective and fast analytical techniques. Among biosensors, impedance biosensors have significant potential for use as simple and portable devices. These sensors involve application of a small amplitude AC voltage to the sensor electrode and measurement of the in-/out-ofphase current response as a function of frequency integrated with some biorecognition element on the sensing electrodes that can bind to the target, modifying the sensor electrical parameters. However, there are some drawbacks concerning their selectivity, stability, and reproducibility. The aim of this paper is to give a critical overview of literature published during the last decade based on the development issues of impedimetric biosensors and their applicability in water analysis.

show abstract

Disposable electrochemical biosensors for the detection of bacteria in the light of antimicrobial resistance

C. S.,

Kini,

Singh

et al. 2024

Biotech & Bioengineering

View full text Add to dashboard Cite

Persistent and inappropriate use of antibiotics is causing rife antimicrobial resistance (AMR) worldwide. Common bacterial infections are thus becoming increasingly difficult to treat without the use of last resort antibiotics. This has necessitated a situation where it is imperative to confirm the infection to be bacterial, before treating it with antimicrobial speculatively. Conventional methods of bacteria detection are either culture based which take anywhere between 24 and 96 hor require sophisticated molecular analysis equipment with libraries and trained operators. These are difficult propositions for resource limited community healthcare setups of developing or less developed countries. Customized, inexpensive, point‐of‐care (PoC) biosensors are thus being researched and developed for rapid detection of bacterial pathogens. The development and optimization of disposable sensor substrates is the first and crucial step in development of such PoC systems. The substrates should facilitate easy charge transfer, a high surface to volume ratio, be tailorable by the various bio‐conjugation chemistries, preserve the integrity of the biorecognition element, yet be inexpensive. Such sensor substrates thus need to be thoroughly investigated. Further, if such systems were made disposable, they would attain immunity to biofouling. This article discusses a few potential disposable electrochemical sensor substrates deployed for detection of bacteria for environmental and healthcare applications. The technologies have significant potential in helping reduce bacterial infections and checking AMR. This could help save lives of people succumbing to bacterial infections, as well as improve the overall quality of lives of people in low‐ and middle‐income countries.

show abstract

Impedimetric paper-based biosensor for the detection of bacterial contamination in water

Cited by 103 publications

References 43 publications

Paper-Based Biosensors for Analysis of Water

Paper-Based Biosensors for Analysis of Water

Challenges and Applications of Impedance-Based Biosensors in Water Analysis

Disposable electrochemical biosensors for the detection of bacteria in the light of antimicrobial resistance

Contact Info

Product

Resources

About