Impedimetric immunosensor for the detection of circulating pro-inflammatory monocytes as infection markers

Montrose, Armelle; Cargou, Sébastien; Nepveu, Françoise; Manczak, Rémi; Gué, Anne Marie; Reybier, Karine

doi:10.1016/j.bios.2013.05.025

Cited by 15 publications

(10 citation statements)

References 27 publications

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“…Previous experiments performed on macro-electrodes with different areas (1, 0.5, 0.04 cm²) have demonstrated a strong increase in sensitivity when decreasing electrodes areas (90% of improvement in ∆Z for 0.04 cm² area compare to 1 cm²) without any increase in the noise [13]. This is in agreement with inverse proportionality between charge transfer resistance and the electrode area.…”

Section: Resultssupporting

confidence: 84%

“…The cleaned electrodes were modified as described by Montrose et al [13]. In absence of channels the surfaces were modified through successive deposition of drops of the modifying agents for various incubation times: a mixture of 11-mercaptoundecanoïc acid (MUA) 1 mM and 6mercaptohexanol (MH) 10 mM for 18h/ a mixture of aqueous solutions of N-hydroxysuccinimide (NHS) 15 mM and 1-Ethyl-3-(3-dimethylaminopropyl-carbodiimide (EDC) 75 mM for 30 min / protein G 100 g.mL -1 in PBS 10 mM for 18h / BSA 143 g/L for 30 min and CD14 antibody X mM for 18h.…”

Section: Electrode Modificationmentioning

confidence: 99%

“…In this latter case, the change in the electrode impedance is measured and can be correlated to the amount of trapped cells [9]. Bio-impedance monitoring has already been employed for example to study cellular kinetics [10], cancer drug screening [11], or parasitized cells [12][13][14]. Electrochemical measurements rely on the use of at least 2 electrodes.…”

Section: Introductionmentioning

confidence: 99%

“…In this paper, we report the study of impedimetric microfluidic devices with improved sensitivity targeting the immuno-detection of cells. The sensitivity of the system was evaluated as the capacity of the electrodes to trap monocytes by immune-reaction with CD14 antibody immobilized on the electrode surface [13] and to produce a significant impedance change. Several designs including simple or interdigitated electrodes were tested and also compared to microfluidic measurements.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Improved on-chip impedimetric immuno-detection of subpopulations of cells toward single-cell resolution

Manczak

Fouet

Courson

et al. 2016

Sensors and Actuators B: Chemical

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International audienceImpedance spectroscopy has gained interest for the quantitative detection of specific cells mainly due to a label-free detection and their miniaturization capability required for integration on chip and development of point-of-care diagnostics. In this paper, we report the study of impedimetric microfluidic devices with improved sensitivity targeting the immuno-detection of cells. The sensitivity of our system was evaluated in terms of the capacity of the electrodes to trap monocytes by immune-reaction with CD14 antibody immobilized on micro-electrode surface. All measurements were performed in faradic mode using a redox probe. The sensitivity was evaluated as a function of the impedance increase recorded at 100 Hz caused by the insulating character of the cell trapped on electrodes. Analyses first confirmed that the sensing performances were significantly improved by using microfluidic. This increase could originate from an increase in the probability of cell trapping and a better organization of cells on the electrode due to the laminar flow. The great sensitivity was recorded with interdigitated electrodes for which the influence of the gap value was evaluated. The maximum sensitivity was reached with the smallest inter-electrodes gap tested (50 µm). This performance was in part attributed to the redox cycling taking place between neighboring fingers that was strongly affected when cells were trapped on the electrodes edges. Furthermore we also demonstrate that the slice of cell concentration for which the sensitivity is maximized is correlated to the area of electrodes. Moreover, the smallest area of interdigitated electrode (0.1 mm length) allowed the detection of as low as 5 cells per m

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Section: Resultssupporting

confidence: 84%

Section: Electrode Modificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improved on-chip impedimetric immuno-detection of subpopulations of cells toward single-cell resolution

Manczak

Fouet

Courson

et al. 2016

Sensors and Actuators B: Chemical

Self Cite

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“…Another method for modifying electrode is self-assembly technique which offered a very simple method for the preparation of a highly ordered, reproducible, and ultrathin film suitable for further modification with antibodies. Many papers have been reported that the self-assembled monolayers (SAMs) prepared from alkanethiols containing carboxylic acid [15][16][17] or amine 18,19 in the tail groups were used to immobilize antibody on the electrode surface. However, carboxylic acid or amine groups of SAMs need to be activated by the mixture of 1-(3-(dimethylamino)-propyl)-3-ethylcarbodi-imidehydrochloride (EDC) and N-hydroxy sulfosuccinimide (NHS) or glutaraldehyde before immobilization of antibodies, indicating that the process of antibody immobilization is tedious.…”

Section: Introductionmentioning

confidence: 99%

The Fabrication of Electrochemical Impedance Immunosensor Based on Aldehyde-containing Self-assembled Monolayers for Hepatitis B Surface Antigen Detection

Shen

Zhang

et al. 2016

Electrochemistry

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In this work, thiol aromatic aldehyde was used as a substrate material to be assembled on the electrode surface through Au-S chemical bond, resulting in an aldehyde-containing self-assembled monolayer. The aldehyde groups of this self-assembled monolayer can immobilize antibodies directly through the covalent bonding with amino groups of antibody, in which no additional chemical cross-linker is required. We fabricated an electrochemical impedance immunosensor based on aldehyde-containing self-assembled monolayers for the detection of hepatitis B surface antigen within the range from 0.1 to 70 ng mL −1 with a detection limit of 0.06 ng mL −1 obtained by 3σ. The proposed immunosensor is simple and has a good specificity and reproducibility.

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A review on impedimetric immunosensors for pathogen and biomarker detection

Leva-Bueno

Peyman

Millner

2020

Med Microbiol Immunol

132

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Since the discovery of antibiotics in the first quarter of the twentieth century, their use has been the principal approach to treat bacterial infection. Modernized medicine such as cancer therapy, organ transplantation or advanced major surgeries require effective antibiotics to manage bacterial infections. However, the irresponsible use of antibiotics along with the lack of development has led to the emergence of antimicrobial resistance which is considered a serious global threat due to the rise of multidrug-resistant bacteria (Wang et al. in Antibiotic resistance: a rundown of a global crisis, pp. 1645-1658, 2018). Currently employed diagnostics techniques are microscopy, colony counting, ELISA, PCR, RT-PCR, surface-enhanced Raman scattering and others. These techniques provide satisfactory selectivity and sensitivity (Joung et al. in Sens Actuators B Chem 161:824-831, 2012). Nevertheless, they demand specialized personnel and expensive and sophisticated machinery which can be labour-intensive and time-consuming, (

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Impedimetric immunosensor for the detection of circulating pro-inflammatory monocytes as infection markers

Cited by 15 publications

References 27 publications

Improved on-chip impedimetric immuno-detection of subpopulations of cells toward single-cell resolution

Improved on-chip impedimetric immuno-detection of subpopulations of cells toward single-cell resolution

The Fabrication of Electrochemical Impedance Immunosensor Based on Aldehyde-containing Self-assembled Monolayers for Hepatitis B Surface Antigen Detection

A review on impedimetric immunosensors for pathogen and biomarker detection

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