Impedance study of the hybrid molecule alginate–pyrrole: Demonstration as host matrix for the construction of a highly sensitive amperometric glucose biosensor

Abu-Rabeah, Khalil; Marks, Robert S.

doi:10.1016/j.snb.2008.09.020

Cited by 23 publications

(9 citation statements)

References 25 publications

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“…We speculate that a nanometer-thick coating of the highly charged polymer, PyHA, might have little influence on charge transfer at interfaces. This finding is consistent with a separate study on enzyme-based biosensors in which electrodes coated with a pyrrole-alginate conjugate showed no negative impact on impedance [43]. …”

Section: Discussionsupporting

confidence: 91%

Pyrrole–hyaluronic acid conjugates for decreasing cell binding to metals and conducting polymers

Lee

Schmidt

2010

Acta Biomaterialia

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Surface modification of electrically conductive biomaterials has been studied to improve biocompatibility for a number of applications, such as implantable sensors and microelectrode arrays. In this study, we electrochemically coated electrodes with biocompatible and non-cell adhesive hyaluronic acid (HA) to reduce cellular adhesion for potential use in neural prostheses. To this end, pyrrole-conjugated hyaluronic acid (PyHA) was synthesized and employed for electrochemical coating of platinum, indium-tin-oxide, and polystyrene sulfonate-doped polypyrrole electrodes. This PyHA conjugate consists of (1) a pyrrole moiety that allows the compound to be electrochemically deposited onto a conductive substrate and (2) non-adhesive HA to minimize cell adhesion and to potentially decrease inflammatory tissue responses. Our characterization results showed the presence of a hydrophilic p(PyHA) layer on the modified electrode, and impedance measurements revealed impedance that was statistically the same as the unmodified electrode. We found that the p(PyHA)-coated electrodes minimized adhesion and migration of fibroblasts and astrocytes for a minimum of up to 3 months. Also, the coating was stable in physiological solution for 3 months and also stable against enzymatic degradation by hyaluronidase. These studies suggest that this p(PyHA)-coating has the potential to be used to mask conducting electrodes from adverse glial responses that occur upon implantation. In addition, electrochemical coating with PyHA can be potentially extended for the surface modification of other metallic and conducting substances such as stents and biosensors.

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Section: Discussionsupporting

confidence: 91%

Pyrrole–hyaluronic acid conjugates for decreasing cell binding to metals and conducting polymers

Lee

Schmidt

2010

Acta Biomaterialia

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“…[4][5][6][7][8][9] Fabrication of the enzyme containing biosensor requires immobilization of the enzyme molecule onto/into the electrode surface. There are several strategies for immobilization techniques to obtain GOD biosensor such as crosslinking with glutaraldehyde, [10][11] applying enzyme on an electrode in a gel film, [12][13][14][15][16][17] entrapment or incorporation in a polymer matrix during electropolymerization, 6,[18][19][20] by covalent attachment [21][22] or adsorbing onto electrode surface. [24][25][26] Chitosan (Chi) is a type of natural cationic polymer, which has shown attractive characteristics such as film-forming ability, permeability, and good adhesion.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles

Özyılmaz

Bayram

et al. 2019

ACSi

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GOD was immobilized onto polypyrrole (PPy) or poly(o-anisidine) (POA) coated Pt electrode to construct glucose sensitive biosensor. Because polymer film properties and enzyme activity affect the current response, PPy and POA synthesis conditions and also enzyme immobilization parameters were optimized in detail. The optimal monomer concentrations were determined as 25 and 50 mM for PPy and POA, respectively, whereas scan rate was 50 mV/s for both polymer films. In case of immobilization procedure, the optimal Chitosan (Chi), glucose oxidase (GOD) and glutaraldehyde (GAL) concentrations were determined as 0.5%, 2 mg/ml and 0.05% for PPy and 0.5%, 4 mg/ml and 0.075% for POA, respectively. Zinc oxide nanoparticles (ZnONP) were co-immobilized with GOD enzyme and it was revealed that ZnONP modification enhanced the efficiencies of both electrodes in terms of current responses and stabilities. Nyquist diagrams showed that enzyme electrodes were sensitive to glucose molecule and ZnONP modification improved the sensor efficiency.

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“…There have been attempts at increasing their diagnostic power by finding alternative ways to treat the sample (lab-on-a-chip), increasing sensitivity which could be done chemically (dendrons [2], and more), biologically (avidin-biotin scaffolds) or physically (metal enhanced fluorescence [3]). In the last years, a number of groups have attempted to increase the overall bioassay efficiency by creating biosensor-based devices using the same biological principles but by adding a transducer such as a fiber optic [4], [5], an electrode [6] or a piezoelectric crystal [7]. Reaching the target analyte faster and in an efficient manner, especially in complex media, is also of high interest.…”

Section: -Introductionmentioning

confidence: 99%

Biochip based on arrays of switchable magnetic nano-traps

Vautrin

Vyazmensky

Engel

et al. 2017

Sensors and Actuators B: Chemical

Self Cite

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Magnetic biochips based on switchable magnetic nano-traps were developed and optimized so as to enable faster bio-assays via a reduction of steps and the efficient capture of analytes in a sample test solution. Nano-traps are based on snake shaped magnetic nanowires made by microelectronic technics from a thin magnetic layer. This optimized design ensures high local fields when the trap is activated and reduced stray fields when deactivated with respect to previous designs. Our proof-of-concept model consists to determine the anchorage of synthetic peptides in a bilayer enveloping magnetic beads by revealing their presence via fluorescently labeled immunoglobulins elicited against the native proteins of the said exposed peptides.

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Impedance study of the hybrid molecule alginate–pyrrole: Demonstration as host matrix for the construction of a highly sensitive amperometric glucose biosensor

Cited by 23 publications

References 25 publications

Pyrrole–hyaluronic acid conjugates for decreasing cell binding to metals and conducting polymers

Pyrrole–hyaluronic acid conjugates for decreasing cell binding to metals and conducting polymers

Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles

Biochip based on arrays of switchable magnetic nano-traps

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