Impedance-derived electrochemical capacitance spectroscopy for the evaluation of lectin–glycoprotein binding affinity

Santos, Alexandre Leme Godoy dos; Carvalho, Fernanda C.; Roque-Barreira, Maria Cristina; Bueno, Paulo Roberto

doi:10.1016/j.bios.2014.06.034

Cited by 41 publications

(60 citation statements)

References 22 publications

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“…When target binding events occur, there is a perturbation on the electrochemical activity associated with the C r signal, as illustrated in Fig. 1f, thus enabling sensitive analytical curves to be constructed using C r as an effective transduction signal (Fernandes et al, 2015(Fernandes et al, , 2013Lehr et al, 2014;Marques et al, 2015;Santos and Bueno, 2016;Santos et al, 2014Santos et al, , 2015aSantos et al, , 2015b. In other words, capacitive approach has the advantage of not requiring the redox probe pre-doping of the biological samples, making it particularly appealing for point-of-care applications (Patil et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In other words, capacitive approach has the advantage of not requiring the redox probe pre-doping of the biological samples, making it particularly appealing for point-of-care applications (Patil et al, 2014). Hence the use of ECS in diagnostic assays has been effectively demonstrated within label-free standards and being highly specific to target biomarkers, presenting good reproducibility and high sensitivity (at nano to picomolar ranges) (Fernandes et al, 2015(Fernandes et al, , 2013Lehr et al, 2014;Marques et al, 2015;Santos and Bueno, 2016;Santos et al, 2014Santos et al, , 2015aSantos et al, , 2015b.…”

Section: Introductionmentioning

confidence: 99%

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The capacitive sensing of NS1 Flavivirus biomarker

Cecchetto

Fernandes

Lopes

et al. 2017

Biosensors and Bioelectronics

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NS1 is a biomarker for different Flavivirus diseases such as dengue (DENV), zika (ZIKV) and chikungunya (CHIKV) and was herein selectively quantified by electrochemical capacitive sensing (an impedance-derived capacitance methodology wherein the redox probe is contained in the receptive layer) mainly aiming dengue diagnosis in phosphate buffer saline and blood serum environments (up to the neat level). The capacitive sensing was compared to traditional concurrent impedimetric approach (in which the redox probe is added in the biological solution) and other transient methods stated in the literature regarding figures of merit such as limit of detection, linear range, relative standard deviation and affinity constant. Capacitive and impedimetric assays showed equivalent results for linear range, repeatability, sensitivity and constant of affinity. Nonetheless capacitive assays presented better reproducibility with a relative standard deviation (RSD) of 3±1 and 7±4 (all in percentage) in PBS and serum, respectively, meanwhile for impedimetric assays the RSD values were 9±5 in PBS and 12±6 in serum. Thus, by using capacitive assays, an improvement on the analytical performance was observed with the limit of detection about sixty-fold lower in neat serum (∼0.5ngmL for capacitive over ∼30ngmL for impedimetric assays) compared to traditional electrochemistry methods in general hence demonstrating the superior detection sensitivity for NS1 protein. Accordingly, redox tagged capacitive assays are suitable for the development of multiplex point-of-care neglected diseases sensing applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The capacitive sensing of NS1 Flavivirus biomarker

Cecchetto

Fernandes

Lopes

et al. 2017

Biosensors and Bioelectronics

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“…From this methodology (summarized in the Figure ), it is possible to extract the capacitance and other resistive elements. Briefly, the circuit describes the charging capability of this redox tethered interface, a physical chemistry feature obtained from the redox capacitance term

C_{r}

. This charging fingerprint can be easily obtained converting the impedance spectra to capacitance (Figure ).…”

Section: Resultsmentioning

confidence: 99%

The self‐assembly of redox active peptides: Synthesis and electrochemical capacitive behavior

et al. 2016

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The present work reports on the synthesis of a redox-tagged peptide with self-assembling capability aiming applications in electrochemically active capacitive surfaces (associated with the presence of the redox centers) generally useful in electroanalytical applications. Peptide containing ferrocene (fc) molecular (redox) group (Ac-Cys-Ile-Ile-Lys(fc)-Ile-Ile-COOH) was thus synthesized by solid phase peptide synthesis (SPPS). To obtain the electrochemically active capacitive interface, the side chain of the cysteine was covalently bound to the gold electrode (sulfur group) and the side chain of Lys was used to attach the ferrocene in the peptide chain. After obtaining the purified redox-tagged peptide, the self-assembly and redox capability was characterized by cyclic voltammetry (CV) and electrochemical impedance-based capacitance spectroscopy techniques. The obtained results confirmed that the redox-tagged peptide was successfully attached by forming an electroactive self-assembled monolayer onto gold electrode. The design of redox active self-assembly ferrocene-tagged peptide is predictably useful in the development of biosensor devices precisely to detect, in a label-free platform, those biomarkers of clinical relevance. © 2016 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 357-367, 2016.

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“…It is possible to use SAM-coated Au electrodes modified with an oligosaccharide to detect lectin and influenza hemagglutinin [71,72]. Bueno and coworkers have prepared lectin-immobilized sensors for studying lectin-glycoprotein interactions based on the impedimetric and capacitive responses of the sensors [73,74]. For this purpose, ArtinM lectin was covalently immobilized on a SAM-coated electrode and the binding behavior to HRP and glycoprotein-bearing leukemia cells was studied.…”

Section: Lectin-based Sensors For Glycoproteinsmentioning

confidence: 99%

Recent Progress in Electrochemical Biosensors for Glycoproteins

Akiba

Anzai

2016

Sensors

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This review provides an overview of recent progress in the development of electrochemical biosensors for glycoproteins. Electrochemical glycoprotein sensors are constructed by combining metal and carbon electrodes with glycoprotein-selective binding elements including antibodies, lectin, phenylboronic acid and molecularly imprinted polymers. A recent trend in the preparation of glycoprotein sensors is the successful use of nanomaterials such as graphene, carbon nanotube, and metal nanoparticles. These nanomaterials are extremely useful for improving the sensitivity of glycoprotein sensors. This review focuses mainly on the protocols for the preparation of glycoprotein sensors and the materials used. Recent improvements in glycoprotein sensors are discussed by grouping the sensors into several categories based on the materials used as recognition elements.

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Impedance-derived electrochemical capacitance spectroscopy for the evaluation of lectin–glycoprotein binding affinity

Cited by 41 publications

References 22 publications

The capacitive sensing of NS1 Flavivirus biomarker

The capacitive sensing of NS1 Flavivirus biomarker

The self‐assembly of redox active peptides: Synthesis and electrochemical capacitive behavior

Recent Progress in Electrochemical Biosensors for Glycoproteins

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