Impedance-Based Nanoporous Anodized Alumina/ITO Platforms for Label-Free Biosensors

Devarakonda, Sivaranjani; Ganapathysubramanian, Baskar; Shrotriya, Pranav

doi:10.1021/acsami.1c17243

Cited by 10 publications

(13 citation statements)

References 42 publications

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“…The temperature of different formulations was recorded using a thermal imager. To measure the photoelectric performance of formulations (P6, PLT, PEDOT, and P6@PEDOT@PLT), the samples were deposited on an indium tin oxide (ITO)-coated glass by oven-drying . The sample-deposited ITO glass was repeatedly irradiated by NIR (1.0 W/cm 2 , on/off: 20/20 s), with the generated electric current being measured by a digital multimeter (DMM-93B + ).…”

Section: Methodsmentioning

confidence: 99%

Biomimetic Platelet Nanomotors for Site-Specific Thrombolysis and Ischemic Injury Alleviation

Chen

Liu

Pan

et al. 2023

ACS Appl. Mater. Interfaces

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Due to the mortality associated with thrombosis and its high recurrence rate, there is a need to investigate antithrombotic approaches. Noninvasive site-specific thrombolysis is a current approach being used; however, its usage is characterized by the following limitations: low targeting efficiency, poor ability to penetrate clots, rapid half-life, lack of vascular restoration mechanisms, and risk of thrombus recurrence that is comparable to that of traditional pharmacological thrombolysis agents. Therefore, it is vital to develop an alternative technique that can overcome the aforementioned limitations. To this end, a cotton-ball-shaped platelet (PLT)-mimetic self-assembly framework engineered with a phototherapeutic poly(3,4-ethylenedioxythiophene) (PEDOT) platform has been developed. This platform is capable of delivering a synthetic peptide derived from hirudin P6 (P6) to thrombus lesions, forming P6@PEDOT@PLT nanomotors for noninvasive site-specific thrombolysis, effective anticoagulation, and vascular restoration. Regulated by P-selectin mediation, the P6@PEDOT@PLT nanomotors target the thrombus site and subsequently rupture under near-infrared (NIR) irradiation, achieving desirable sequential drug delivery. Furthermore, the movement ability of the P6@PEDOT@PLT nanomotors under NIR irradiation enables effective penetration deep into thrombus lesions, enhancing bioavailability. Biodistribution analyses have shown that the administered P6@PEDOT@PLT nanomotors exhibit extended circulation time and metabolic capabilities. In addition, the photothermal therapy/photoelectric therapy combination can significantly augment the effectiveness (ca. 72%) of thrombolysis. Consequently, the precisely delivered drug and the resultant phototherapeutic-driven heat-shock protein, immunomodulatory, anti-inflammatory, and inhibitory plasminogen activator inhibitor-1 (PAI-1) activities can restore vessels and effectively prevent rethrombosis. The described biomimetic P6@PEDOT@PLT nanomotors represent a promising option for improving the efficacy of antithrombotic therapy in thrombus-related illnesses.

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Section: Methodsmentioning

confidence: 99%

Biomimetic Platelet Nanomotors for Site-Specific Thrombolysis and Ischemic Injury Alleviation

Chen

Liu

Pan

et al. 2023

ACS Appl. Mater. Interfaces

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“…The results showed that the sensing response is produced by modified ionic transport under the effect of steric hindrance and surface charge modification induced by ligand–receptor attachment between thrombin and the aptamer-covered alumina film. The constructed sensor detected TB with a detection limit of 10 pM and offered production simplicity, cost-effectiveness, and miniaturization [ 62 ].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Recent Progresses in Development of Biosensors for Thrombin Detection

et al. 2022

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Thrombin is a serine protease with an essential role in homeostasis and blood coagulation. During vascular injuries, thrombin is generated from prothrombin, a plasma protein, to polymerize fibrinogen molecules into fibrin filaments. Moreover, thrombin is a potent stimulant for platelet activation, which causes blood clots to prevent bleeding. The rapid and sensitive detection of thrombin is important in biological analysis and clinical diagnosis. Hence, various biosensors for thrombin measurement have been developed. Biosensors are devices that produce a quantifiable signal from biological interactions in proportion to the concentration of a target analyte. An aptasensor is a biosensor in which a DNA or RNA aptamer has been used as a biological recognition element and can identify target molecules with a high degree of sensitivity and affinity. Designed biosensors could provide effective methods for the highly selective and specific detection of thrombin. This review has attempted to provide an update of the various biosensors proposed in the literature, which have been designed for thrombin detection. According to their various transducers, the constructions and compositions, the performance, benefits, and restrictions of each are summarized and compared.

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“…Electrochemical impedance spectroscopy can measure target concentrations via changes in the degree of crowding on electrode interface, avoiding tedious steps for addition and preservation of labeled probes, making detection operations and device integration much more accessible for further bedside diagnosis of infectious diseases. , However, in planar electrodes, due to the low molecular weight of nucleic acids, there is little change in the degree of crowding before and after nucleic acid hybridization, resulting in an unobvious change in impedance, limiting the utility of electrochemical impedance spectroscopy for infectious disease nucleic acid testing. , A growing body of evidence suggests that the rigidity of nucleic acids can be enhanced greatly by forming a double-stranded complex, and their molecular volume would change more by confined structures of electrode interface. − Inspired by this, the nanopore structure with a high surface area was introduced into the electrodes . Nucleic acids can block the pore by forming a rigid double-stranded structure, and this structure sharply reduces the real surface area of nanopore electrodes, thus amplifying changes of impedance value and addressing the sensitivity limitations of impedance methods toward detecting small nucleic acid molecules. − …”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Viral Nucleic Acids by DNA Nanolock-Based Porous Electrode Device

Huang,

Wang,

Wang

et al. 2023

Anal. Chem.

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Developing rapid, sensitive, and facile nucleic acid detection technologies is of paramount importance for preventing and controlling infectious diseases. Benefiting from the advantages such as rapid response, low cost, and simple operation, electrochemical impedance spectroscopy holds great promise for point-of-care nucleic acid detection. However, the sensitivity of electrochemical impedance spectroscopy for low molecular weight nucleic acids testing is still limited. This work presents a DNA nanolock-based porous electrode to improve the sensitivity of electrochemical impedance spectroscopy. Once the target nucleic acids are recognized by the DNA probes, the pore-attached DNA nanolock caused remarkable impedance amplification by blocking the nanopores. Taking SARS-CoV-2 nucleic acid as a model analyte, the detection limit of the porous electrode was as low as 0.03 fM for both SARS-CoV-2 RNA and DNA. The integration of a porous electrode with a wireless communicating unit generates a portable detection device that could be applied to direct SARS-CoV-2 nucleic acid testing in saliva samples. The portable device could effectively distinguish the COVID-19 positive and negative samples, showing a sensitivity of 100% and a specificity of 93%. Owing to its rapid, ultrasensitive, specific, and portable features, the as-designed DNA nanolock and porous electrode-based portable device holds great promise as a point-of-care platform for real-time screening of COVID-19 and other epidemics.

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Impedance-Based Nanoporous Anodized Alumina/ITO Platforms for Label-Free Biosensors

Cited by 10 publications

References 42 publications

Biomimetic Platelet Nanomotors for Site-Specific Thrombolysis and Ischemic Injury Alleviation

Biomimetic Platelet Nanomotors for Site-Specific Thrombolysis and Ischemic Injury Alleviation

Recent Progresses in Development of Biosensors for Thrombin Detection

Electrochemical Detection of Viral Nucleic Acids by DNA Nanolock-Based Porous Electrode Device

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