Impedance based detection of pathogenic E. coli O157:H7 using a ferrocene-antimicrobial peptide modified biosensor

Li, Yongxin; Afrasiabi, Rouzbeh; Fathi, Farkhondeh; Wang, Nan; Xiang, Cuili; Love, Ryan J.; She, Zhe; Kraatz, Heinz‐Bernhard

doi:10.1016/j.bios.2014.02.045

Cited by 137 publications

(81 citation statements)

References 40 publications

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“…In another study, an immunosensor for the ultrasensitive detection of E. coli O157:H7 on biofunctional magnetic beads was reported to determine the bacterial cell concentration in a nanoporous alumina membrane . E. coli O157:H7 can also be detected using biosensors that employ a ferrocene-antimicrobial peptide conjugate on a gold surface based on impedance (Li et al, 2014).…”

Section: Escherichia Colimentioning

confidence: 99%

Biosensors and their Applications in Food Safety: A Review

Yasmin¹,

Ahmed²,

Cho³

2016

Journal of Biosystems Engineering

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Background: Foodborne pathogens are a growing concern with respect to human illnesses and death. There is an increasing demand for improvements in global food safety. However, it is a challenge to detect and identify these harmful organisms in a rapid, responsive, suitable, and effective way. Results: Rapid developments in biosensor designs have contributed to the detection of foodborne pathogens and other microorganisms. Biosensors can automate this process and have the potential to enable fast analyses that are cost and time-effective. Various biosensor techniques are available that can identify foodborne pathogens and other health hazards. Conclusions: In this review, biosensor technology is briefly discussed, followed by a summary of foodborne pathogen detection using various transduction systems that exhibit specificity for particular foodborne pathogens. In addition, the recent application of biosensor technology to detect pesticides and heavy metals is briefly addressed.

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Section: Escherichia Colimentioning

confidence: 99%

Biosensors and their Applications in Food Safety: A Review

Yasmin¹,

Ahmed²,

Cho³

2016

Journal of Biosystems Engineering

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“…Consequently, different miniaturized sensors platforms were developed in order to capture specific bacteria and to control microbial infections by using particular immobilized antibodies. For example, we can cite the acoustic wave (Leonard et al 2003), the surface plasmon resonance (SPR) (Wang et al 2012), the potentiometric (Hernandez et al 2014), the conductometric (Liu et al 2009), the amperometric biosensor (Tang et al 2006), the colorimetric (Pires et al 2011), and the impedance bacteria biosensors (Li et al 2014;Varshney and Li 2009).…”

Section: Introductionmentioning

confidence: 99%

An investigation of the well-water quality: immunosensor for pathogenic Pseudomonas aeruginosa detection based on antibody-modified poly(pyrrole-3 carboxylic acid) screen-printed carbon electrode

Békir

Bousimma

Barhoumi

et al. 2015

Environ Sci Pollut Res

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In this report, we describe a new immunosensor designed for the detection and the quantification of Pseudomonas aeruginosa bacteria in water. The developed biosensing system was based on the immobilization of purified polyclonal anti P. aeruginosa antibodies on electropolymerized poly(pyrrole-3-carboxylic acid)/glassy carbon electrode. The building of the immunosensor step by step was evaluated by electrochemical measurements such as cyclic voltammetry (CV) and impedance spectroscopy (EIS). The electrochemical signature of the immunosensor was established by the change of the charge transfer resistance when the bacteria suspended in solution became attached to the immobilized antibodies. As a result, stable and high sensitive impedimetric immunosensor was obtained with a sensitivity of 0.19 kΩ/decade defined in the linear range from 10(1) to 10(7) CFU/mL of cellular concentrations. A low detection limit was obtained for the P. aeruginosa bacteria and a high selectivity when other bacteria were occasioned as well as Escherichia coli. The developed immunosensor was applied in detecting pathogenic P. aeruginosa in well-water.

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“…In particular, impedance based electrical transduction, where the applied electrical signal is alternating as opposed to direct, can be used to analyze both the resistive and the capacitive changes at the electrode surface over a wide frequency range during affinity binding. This analytical approach, known as electrochemical impedance spectroscopy (EIS), is an effective strategy for the non-destructive probing of complex bio-recognition events [6,7] and has been utilized in a wide range of biosensors ranging from bacteria and pathogen detection to immunosensing and DNA characterization [8][9][10][11]. When a target analyte is captured by the receptor functionalized electrode surface, it alters the electrical properties (e.g.…”

Section: Why Impedance Biosensors For Point Of Care Diagnostics?mentioning

confidence: 99%