Impaired Wound Induction of 3-Deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) Synthase and Altered Stem Development in Transgenic Potato Plants Expressing a DAHP Synthase Antisense Construct

Jones, James D.; Henstrand, John M.; Handa, Avtar K.; Herrmann, Klaus M.; Weller, Stephen C.

doi:10.1104/pp.108.4.1413

Cited by 40 publications

(17 citation statements)

References 43 publications

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“…4a) and following wounding (Fig. 4b) are consistent with increased levels of expression to allow synthesis of secondary metabolites originating in the shikimate pathway [4,11,12,17]. The high levels of transaldolase mRNA observed in these tissues suggest a transcriptional response to an increased demand for erythrose 4-phosphate, accommodating increased flux through the shikimate pathway [4,13,17].…”

Section: Discussionsupporting

confidence: 48%

Cloning and expression of transaldolase from potato

et al. 1996

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We have isolated a cDNA encoding transaldolase, an enzyme of the pentose-phosphate pathway, from potato (Solanum tuberosum). The 1.5 kb cDNA encodes a protein of 438 amino acid residues with a molecular mass of 47.8 kDa. When the potato cDNA was expressed in Escherichia coli a 45 kDa protein with transaldolase activity was produced. The first 62 amino acids of the deduced amino acid sequence represent an apparent plastid transit sequence. While the potato transaldolase has considerable similarity to the enzyme from cyanobacteria and Mycobacterium leprae, similarity to the conserved transaldolase enzymes from humans, E. coli and Saccharomyces cerevisiae is more limited. Northern analysis indicated that the transaldolase mRNA accumulated in tubers in response to wounding. Probing the RNA from various potato tissues indicated that the transaldolase mRNA accumulation to higher levels in the stem of mature potato plants than in either leaves or tubers. These data are consistent with a role for this enzyme in lignin biosynthesis.

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Section: Discussionsupporting

confidence: 48%

Cloning and expression of transaldolase from potato

et al. 1996

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“…It has been demonstrated that compromising the shikimate pathway, by the inhibition of DAHPS or DHD/SDH, leads to stunted growth and reduction in some secondary metabolites, such as lignin and chlorogenate, in potato and tobacco plants (Jones et al 1995;Ding et al 2007). Other studies have also documented the importance of this pathway in the synthesis of plant secondary metabolites (Lillo et al 2007).…”

Section: Discussionmentioning

confidence: 98%

Expression balances of structural genes in shikimate and flavonoid biosynthesis cause a difference in proanthocyanidin accumulation in persimmon (Diospyros kaki Thunb.) fruit

Akagi

Ikegami

Suzuki

et al. 2009

Planta

102

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Persimmon fruits accumulate a large amount of proanthocyanidin (PA) during development. Fruits of pollination-constant and non-astringent (PCNA) type mutants lose their ability to produce PA at an early stage of fruit development, while fruits of the normal (non-PCNA) type remain rich in PA until fully ripened. To understand the molecular mechanism for this difference, we isolated the genes involved in PA accumulation that are differentially expressed between PCNA and non-PCNA, and confirmed their correlation with PA content and composition. The expression of structural genes of the shikimate and flavonoid biosynthetic pathways and genes encoding transferases homologous to those involved in the accumulation of phenolic compounds were downregulated coincidentally only in the PCNA type. Analysis of PA composition using the phloroglucinol method suggested that the amounts of epigallocatechin and its 3-O-gallate form were remarkably low in the PCNA type. In the PCNA type, the genes encoding flavonoid 3'5' hydroxylase (F3'5'H) and anthocyanidin reductase (ANR) for epigallocatechin biosynthesis showed remarkable downregulation, despite the continuous expression level of their competitive genes, flavonoid 3' hydroxylation (F3'H) and leucoanthocyanidin reductase (LAR). We also confirmed that the relative expression levels of F3'5'H to F3'H, and ANR to LAR, were considerably higher, and the PA composition corresponded to the seasonal expression balances in both types. These results suggest that expressions of F3'5'H and ANR are important for PA accumulation in persimmon fruit. Lastly, we tested enzymatic activity of recombinant DkANR in vitro, which is thought to be an important enzyme for PA accumulation in persimmon fruits.

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“…Several studies reveal the reverse, namely, consequences of downregulation or disruption of enzyme functions in primary metabolism for secondary metabolism. For example, downregulation of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (shikimate pathway) or plastid transketolase (TK, Figure 7) leads to a decrease in aromatic amino acids, phenylpropanoids, and lignin (Jones et al, 1995;Henkes et al, 2001). On the contrary, here, far-reaching consequences for primary metabolism caused by a disruption in an enzyme of secondary metabolism have been found at the transcriptional level (Figure 7).…”

Section: A Reduced Carbon Flux Into the Phenylpropanoid Pathway Affecmentioning

confidence: 99%

Molecular Phenotyping of the pal1 and pal2 Mutants of Arabidopsis thaliana Reveals Far-Reaching Consequences on Phenylpropanoid, Amino Acid, and Carbohydrate Metabolism

et al. 2004

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The first enzyme of the phenylpropanoid pathway, Phe ammonia-lyase (PAL), is encoded by four genes in Arabidopsis thaliana. Whereas PAL function is well established in various plants, an insight into the functional significance of individual gene family members is lacking. We show that in the absence of clear phenotypic alterations in the Arabidopsis pal1 and pal2 single mutants and with limited phenotypic alterations in the pal1 pal2 double mutant, significant modifications occur in the transcriptome and metabolome of the pal mutants. The disruption of PAL led to transcriptomic adaptation of components of the phenylpropanoid biosynthesis, carbohydrate metabolism, and amino acid metabolism, revealing complex interactions at the level of gene expression between these pathways. Corresponding biochemical changes included a decrease in the three major flavonol glycosides, glycosylated vanillic acid, scopolin, and two novel feruloyl malates coupled to coniferyl alcohol. Moreover, Phe overaccumulated in the double mutant, and the levels of many other amino acids were significantly imbalanced. The lignin content was significantly reduced, and the syringyl/guaiacyl ratio of lignin monomers had increased. Together, from the molecular phenotype, common and specific functions of PAL1 and PAL2 are delineated, and PAL1 is qualified as being more important for the generation of phenylpropanoids.

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Impaired Wound Induction of 3-Deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) Synthase and Altered Stem Development in Transgenic Potato Plants Expressing a DAHP Synthase Antisense Construct

Cited by 40 publications

References 43 publications

Cloning and expression of transaldolase from potato

Cloning and expression of transaldolase from potato

Expression balances of structural genes in shikimate and flavonoid biosynthesis cause a difference in proanthocyanidin accumulation in persimmon (Diospyros kaki Thunb.) fruit

Molecular Phenotyping of the pal1 and pal2 Mutants of Arabidopsis thaliana Reveals Far-Reaching Consequences on Phenylpropanoid, Amino Acid, and Carbohydrate Metabolism

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