Impaired Neutrophil Function in Patients With AIDS or AIDS-Related Complex: A Comprehensive Evaluation

Ellis, Mark; Gupta, Sudhir; Galant, Stanley P.; Hakim, Said; vandeVen, Carmella; Toy, Cindi; Cairo, Mitchell S.

doi:10.1093/infdis/158.6.1268

Cited by 238 publications

(87 citation statements)

References 25 publications

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“…It is possible that this unresponsiveness is a re¯ection of stunted development of the immune system in perinatally HIV-infected children. In fact, the absence of any increase in the MFI of the newly expressed CD64 in the paediatric HIV patients' PMNs and the complete absence of any responses in the expression of other immunologically important receptors CD16, CD32 and CD11b throughout all classes of perinatal HIV infection in this study clearly differ from the ®ndings reported in studies on adult ARC [9,11]. In our view, these differences strongly favour the possibility of a stunting effect of the perinatally transmitted HIV infection on the development of the immune system.…”

Section: Fluorescent Staining Of Pmnscontrasting

confidence: 92%

Expression of Fcγr1 (CD64) on Polymorphonuclear Leucocytes during Progression to Acquired Immunodeficiency Syndrome in Perinatally Human Immunodeficiency Virus‐Infected Children

Moallem

Kalaycı

Homel³

et al. 2000

Scand J Immunol

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CD64, the high‐affinity receptor in the family of FCγ receptors, is not expressed constitutively in polymorphonuclear leucocytes (PMN). CD64 is expressed by PMNs in the late stages of human immunodeficiency virus (HIV) infection in adults. We followed the expression of CD64 on PMNs in perinatally HIV‐infected children during disease progression. Peripheral blood leucocytes (PBL) from 45 perinatally HIV‐infected paediatric patients and 13 healthy age‐matched controls were analysed using cytofluorimetry after reaction with a fluorophore‐labelled monoclonal antibody (MoAb) to CD64. In parallel, we examined the expression of CD32, CD16, CD11b and the human neutrophil‐specific BH2‐Ag using fluorophore‐labelled MoAbs. We found that up to 79.5% of the PMNs in children in class C3 express CD64. Most importantly, we observed a continuous and significant increase in the appearance of CD64+ PMNs as a function of CDC classification (P < 0.001) but no changes in the expression of CD32, CD16, CD11b and BH2‐Ag. This suggests that following the expression of CD64 on PMNs can be useful in evaluating the progression of HIV infection in perinatally HIV‐infected children.

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Section: Fluorescent Staining Of Pmnscontrasting

confidence: 92%

Expression of Fcγr1 (CD64) on Polymorphonuclear Leucocytes during Progression to Acquired Immunodeficiency Syndrome in Perinatally Human Immunodeficiency Virus‐Infected Children

Moallem

Kalaycı

Homel³

et al. 2000

Scand J Immunol

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“…gp41 has been detected in brain tissues of patients affected by AIDS dementia (22) and Abs recognizing various epitopes of gp41 appear at early stages of HIV infection (61). Moreover, it has been reported that monocytes and neutrophils from HIV-1-infected patients responded poorly to a variety of chemoattractants, including FMLP (62)(63)(64)(65)(66). The interaction of gp41 peptides with FPRs on human basophils might be of clinical relevance in patients with HIV-1 infection.…”

Section: Increasing Evidence Indicates That Human Fc⑀rimentioning

confidence: 99%

HIV-1 Envelope gp41 Peptides Promote Migration of Human FcεRI+ Cells and Inhibit IL-13 Synthesis Through Interaction with Formyl Peptide Receptors

Paulis

Florio

Prevete

et al. 2002

The Journal of Immunology

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We evaluated the effects of synthetic peptides (2017, 2019, 2020, 2021, 2023, 2027, 2029, 2030, 2031, and 2035) encompassing the structure of HIV-1MN envelope gp41 on both chemotaxis of human basophils and the release of preformed mediators (histamine) and of cytokines (IL-13). Peptides 2019 and 2021 were potent basophil chemoattractants, whereas the other peptides examined were ineffective. Preincubation of basophils with FMLP or gp41 2019 resulted in complete desensitization to a subsequent challenge with homologous stimulus. Incubation of basophils with low concentration (5 × 10−7 M) of FMLP, which binds with high affinity to N-formyl peptide receptor (FPR), but not to FPR-like 1, did not affect the chemotactic response to a heterologous stimulus (gp41 2019). In contrast, a high concentration (10−4 M) of FMLP, which binds also to FPR-like 1, significantly reduced the chemotactic response to gp41 2019. The FPR antagonist cyclosporin H inhibited chemotaxis induced by FMLP, but not by gp41 2019. None of these peptides singly induced the release of histamine or cytokines (IL-4 and IL-13) from basophils. However, low concentrations of peptides 2019 and 2021 (10−8–10−6 M) inhibited histamine release from basophils challenged with FMLP but not the secretion caused by anti-IgE and gp120. Preincubation of basophils with peptides 2019 and 2021 inhibited the expression of both IL-13 mRNA, and the FMLP-induced release of IL-13 from basophils. These data highlight the complexity of the interactions between viral and bacterial peptides with FPR subtypes on human basophils.

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“…Although B cell abnormalities are recognized [2], neutrophil dysfunction is also well described [3]. Published data on neutrophil function in HIV infection are difficult to interpret, as results are inconsistent and often conflicting due to differences in method, in study populations and in concomitant medication [3][4][5][6][7]. Abnormal neutrophil chemotaxis is the most commonly described defect for most HIV þ groups.…”

Section: Introductionmentioning

confidence: 99%

Neutrophil adhesion molecules in HIV disease

Moore

Henderson

Gazzard

1998

Clinical and Experimental Immunology

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Neutrophil dysfunction in HIV disease is well described. We examined the expression of neutrophil adhesion molecules amongst 72 HIV‐infected subjects using a whole blood flow cytometric assay with FITC‐ and R‐PE‐labelled isotype‐specific MoAbs. We report lesser expression of CD11a (LFA‐1) and L‐selectin (CD62L) on the circulating neutrophils of HIV+ subjects compared with HIV− controls. Expression of CD11b (Mac‐1) was unchanged. Shedding of L‐selectin and up‐regulation of CD11b in response to in vitro stimulation with N‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP) were less in HIV+ compared with HIV− subjects, most markedly in subjects with CD4 cell counts < 100 cells/mm3. These results suggest that neutrophil dysfunction in HIV disease, which increases with disease progression, may be attributable to dysregulated adhesion molecule expression.

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Impaired Neutrophil Function in Patients With AIDS or AIDS-Related Complex: A Comprehensive Evaluation

Cited by 238 publications

References 25 publications

Expression of Fcγr1 (CD64) on Polymorphonuclear Leucocytes during Progression to Acquired Immunodeficiency Syndrome in Perinatally Human Immunodeficiency Virus‐Infected Children

Expression of Fcγr1 (CD64) on Polymorphonuclear Leucocytes during Progression to Acquired Immunodeficiency Syndrome in Perinatally Human Immunodeficiency Virus‐Infected Children

HIV-1 Envelope gp41 Peptides Promote Migration of Human FcεRI+ Cells and Inhibit IL-13 Synthesis Through Interaction with Formyl Peptide Receptors

Neutrophil adhesion molecules in HIV disease

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