Expression of Fcγr1 (CD64) on Polymorphonuclear Leucocytes during Progression to Acquired Immunodeficiency Syndrome in Perinatally Human Immunodeficiency Virus‐Infected Children

Abstract

CD64, the high‐affinity receptor in the family of FCγ receptors, is not expressed constitutively in polymorphonuclear leucocytes (PMN). CD64 is expressed by PMNs in the late stages of human immunodeficiency virus (HIV) infection in adults. We followed the expression of CD64 on PMNs in perinatally HIV‐infected children during disease progression. Peripheral blood leucocytes (PBL) from 45 perinatally HIV‐infected paediatric patients and 13 healthy age‐matched controls were analysed using cytofluorimetry after reaction with a fluorophore‐labelled monoclonal antibody (MoAb) to CD64. In parallel, we examined the expression of CD32, CD16, CD11b and the human neutrophil‐specific BH2‐Ag using fluorophore‐labelled MoAbs. We found that up to 79.5% of the PMNs in children in class C3 express CD64. Most importantly, we observed a continuous and significant increase in the appearance of CD64+ PMNs as a function of CDC classification (P < 0.001) but no changes in the expression of CD32, CD16, CD11b and BH2‐Ag. This suggests that following the expression of CD64 on PMNs can be useful in evaluating the progression of HIV infection in perinatally HIV‐infected children.

Neutrophil CD64 Expression as a Diagnostic Marker of Bacterial Infection in Febrile Children Presenting to a Hospital Emergency Department

“…Fjaertoft and colleagues 9 reported increased neutrophil CD64 expression in patients with influenza A infection, especially in those with complications. Moallem and colleagues 19 found a significant increase in neutrophil CD64 expression as perinatally infected human immunodeficiency virusYinfected children progressed to acquired immunodeficiency syndrome. The increased CD64 expression observed in these 2 studies might be explained by a significant increase in interferon-+ production in these viral infections.…”

Impact of T cell activation, HIV replication and hepatitis C virus infection on neutrophil CD64 expression

“…In these ART-treated HIV-infected adults, immune activation and persistent inflammation fuel non-AIDS-defining events such as cardiovascular disease and non-AIDS-defining malignancies. CD64 expression could be correlated to HIV stage disease in infants perinatally infected by HIV (25). Selective defects of neutrophil functions have been also reported with relation to HIV infection (23).…”

“…These impairments of innate response in HIV infected individuals may be involved in the increased susceptibility to bacterial infections observed despite immune reconstitution on ART (24). CD64 expression could be correlated to HIV stage disease in infants perinatally infected by HIV (25).…”

Are Neutrophil CD64 Expression and Interleukin-6 Early Useful Markers for Diagnosis of Acute Appendicitis?

“…[11][12][13] Other studies have reported a similar association between elevation in polymorphonuclear neutrophil CD64 expression and the presence of infection. [14][15][16][17][18][19][20][21][22][23] However, neutrophil CD64 expression is not used for diagnosis of AA yet.…”