Impact of T cell activation, HIV replication and hepatitis C virus infection on neutrophil CD64 expression

Morquin, David; Tuaillon, Édouard; Makinson, Alain; Bendriss, Sophie; Moing, Vincent Le; Reynes, Jacques

doi:10.1002/cyto.b.21385

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Furthermore, our in vitro observations also allowed us to dissect the specific effect of IFN-γ, IFN-I and TNF-α on the enhancement of the functional properties of IC-activated neutrophils and monocytes. Notably, IFN-γ and IFN-I priming (but not TNF-α), led to the upregulation of FcγRs in neutrophils and/or monocytes, in agreement with previous reports in other experimental settings (Dahal et al, 2017; Lehmann et al, 2017; Morquin et al, 2017; Pricop et al, 2001). Consistent with these results, our in vivo work shows that the inflammatory environment resulting from ongoing infection and mAb-treatment also modulates FcγRs expression on neutrophils and monocytes (in particular FcγRIV).…”

Section: Discussionsupporting

confidence: 92%

Differential and sequential immunomodulatory role of neutrophils and Ly6C^hiinflammatory monocytes during antiviral antibody therapy

Lambour¹,

Naranjo-Gómez

Boyer-Clavel³

et al. 2020

Preprint

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46Antiviral monoclonal antibodies (mAbs) can generate protective immunity through immune 47 complexes (IC)-FcgRs interactions. We have shown the essential role of neutrophils in mAb-induced 48 immunity of retrovirus-infected mice. Using this model, here we addressed how viral infection, with 49 or without mAb therapy, affects neutrophils' functional activation. We found that neutrophils 50 activated by viral ICs secreted high levels of chemokines able to recruit monocytes and neutrophils 51 themselves. Moreover, inflammatory cytokines potentiated chemokines and cytokines release by IC-52 activated cells and induced FcγRs upregulation. Similarly, infection and mAb-treatment upregulated 53 FcγRs expression on neutrophils and enhanced their cytokines and chemokines secretion. FcγRs 54 upregulation allowed to identify in vivo two splenic neutrophils subpopulations with distinct 55 phenotypic and functional properties that differentially and sequentially collaborate with inflammatory 56 monocytes to induce Th1-type responses in mAb-treated mice. Our work provides novel findings on 57 the heterogeneity and the immunomodulatory role of neutrophils in the enhancement of immune 58 responses upon antiviral mAb therapy. 59 60 61 62 102 to multiple FcgR-expressing cells such as DCs and neutrophils. While the role of IC-activated DCs in 103 the enhancement of antiviral immune responses has been addressed in several studies (Lambour et al., 104 2016; Wang et al., 2019; Wen et al., 2016) the role of IC-activated neutrophils has mostly been 105 overlooked. Evidence shows that neutrophils, in addition to being key effector cells to fight against 106 invading pathogens, are also endowed with immunomodulatory properties through the secretion of a 107 plethora of chemokines and cytokines (Mantovani et al., 2011; Tamassia et al., 2018; Tecchio and 108 Cassatella, 2016). Yet, the functional activation of neutrophils by viral ICs and the resulting effect on 109 their immunomodulatory properties have poorly been studied in the context of antiviral mAbs 110 therapies. Furthermore, recent studies have highlighted a vast heterogeneity of neutrophils phenotypes 111 and functions in both homeostatic and pathological conditions (Deniset and Kubes, 2018; Ng et al., 112 2019; Scapini et al., 2016; Silvestre-Roig et al., 2016, 2019). As these phenotypic and functional 113 variants of neutrophils depend on their context-dependent stimulation (inflammatory environment, 114 timing, disease progression, …), it is important to dissect how the viral infection and mAb therapy 115 shape the phenotype and functional properties of neutrophils. 116 117 Here, we used the FrCasE retroviral model to address how viral infection, with or without mAb 118 therapy, affects the phenotypical and functional activation of neutrophils. Neutrophils activated by 119 viral determinants secreted high levels of monocytes-and neutrophils-recruiting chemokines. We have 120 shown that the inflammatory environment resulting from the ongoing infection and mAb-treatment 121 modul...

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Section: Discussionsupporting

confidence: 92%

Differential and sequential immunomodulatory role of neutrophils and Ly6C^hiinflammatory monocytes during antiviral antibody therapy

Lambour¹,

Naranjo-Gómez

Boyer-Clavel³

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…A flow cytometric application to study the impact of T‐cell activation associated with viral co‐infection with HIV and hepatitis C. Morquin, et al describe CD64 expression on neutrophils and developed a method to study the role of dual viral infection on this cell type using a protocol derived from one used for neonatal sepsis . The objective was to associate neutrophil CD64 expression with CD8 T‐cell activation, CD4 T‐cells numbers, and relate the above to HIV and HCV replications.…”

Section: Neutrophil Cd64 Expression and Co‐infection With Hiv And Hcvmentioning

confidence: 99%

Issue Highlights ‐ November 2017 (92:B6)

Kestens¹,

Mandy

2017

Cytometry Part B Clinical

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The role of polymorphonuclear neutrophils during HIV-1 infection

et al. 2017

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It is well-recognized that human immunodeficiency virus type-1 (HIV-1) mainly targets CD4 T cells and macrophages. Nonetheless, during the past three decades, a huge number of studies have reported that HIV-1 can directly or indirectly target other cellular components of the immune system including CD8 T cells, B cells, dendritic cells, natural killer cells, and polymorphonuclear neutrophils (PMNs), among others. PMNs are the most abundant leukocytes in the human circulation, and are known to play principal roles in the elimination of invading pathogens, regulating different immune responses, healing of injured tissues, and maintaining mucosal homeostasis. Until recently, little was known about the impact of HIV-1 infection on PMNs as well as the impact of PMNs on HIV-1 disease progression. This is because early studies focused on neutropenia and recurrent microbial infections, particularly, during advanced disease. However, recent studies have extended the investigation area to cover new aspects of the interactions between HIV-1 and PMNs. This review aims to summarize these advances and address the impact of HIV-1 infection on PMNs as well as the impact of PMNs on HIV-1 disease progression to better understand the pathophysiology of HIV-1 infection.

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Impact of T cell activation, HIV replication and hepatitis C virus infection on neutrophil CD64 expression

Cited by 3 publications

References 29 publications

Differential and sequential immunomodulatory role of neutrophils and Ly6C^hiinflammatory monocytes during antiviral antibody therapy

Differential and sequential immunomodulatory role of neutrophils and Ly6C^hiinflammatory monocytes during antiviral antibody therapy

Issue Highlights ‐ November 2017 (92:B6)

The role of polymorphonuclear neutrophils during HIV-1 infection

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Impact of T cell activation, HIV replication and hepatitis C virus infection on neutrophil CD64 expression

Cited by 3 publications

References 29 publications

Differential and sequential immunomodulatory role of neutrophils and Ly6Chiinflammatory monocytes during antiviral antibody therapy

Differential and sequential immunomodulatory role of neutrophils and Ly6Chiinflammatory monocytes during antiviral antibody therapy

Issue Highlights ‐ November 2017 (92:B6)

The role of polymorphonuclear neutrophils during HIV-1 infection

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Differential and sequential immunomodulatory role of neutrophils and Ly6C^hiinflammatory monocytes during antiviral antibody therapy

Differential and sequential immunomodulatory role of neutrophils and Ly6C^hiinflammatory monocytes during antiviral antibody therapy