Impaired intestinal barrier integrity in the colon of patients with irritable bowel syndrome: involvement of soluble mediators

Piche, Thierry; Barbara, Giovanni; Aubert, P.; Varannes, Stanislas Bruley des; Dainese, Raffaella; Nano, Jean-Louis; Cremon, Cesare; Stanghellini, Vincenzo; Giorgio, Roberto De; Galmiche, Jean–Paul; Neunlist, Michel

doi:10.1136/gut.2007.140806

Cited by 447 publications

(438 citation statements)

References 32 publications

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“…[105][106][107] Studies focused on permeability assessment using urinary recovery of orally administered sugars or radioisotopes (Table 3) reported increased permeability in the small intestine, 22,108,109 with the exception of one study performed in children. 60 Several studies also highlighted an increased colonic permeability 60,71,[109][110][111] ; however, this result could not be consistently replicated by other groups. 108,112 Two groups have examined whether the altered intestinal permeability observed in IBS patients could arise from an improper response of the intestinal barrier to noxious agents rather than intrinsic defects.…”

Section: Intestinal Permeability In Ibs Patientsmentioning

confidence: 72%

“…Finally, recent studies assessing the integrity of the epithelial barrier have observed a decrease in the expression of the tight junction proteins occludin, claudin-1 and zonula occludens-1 in the colon of IBS patients. 76,110,114 Key points: (i) Increased permeability was observed in the small intestine and colon of IBS patients.…”

Section: Intestinal Permeability In Ibs Patientsmentioning

confidence: 99%

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Review article: associations between immune activation, intestinal permeability and the irritable bowel syndrome

Matricon

Méleine

Gelot

et al. 2012

Aliment Pharmacol Ther

183

180

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This uncommissioned review article was subject to full peer-review. SUMMARY BackgroundIrritable bowel syndrome (IBS), one of the most common gastrointestinal disorders, markedly impairing patients' quality of life. Drug development for IBS treatment has been hampered by the lack of understanding of IBS aetiology. In recent years, numerous data have emerged that suggest the involvement of immune activation in IBS, at least in a subset of patients. AimTo determine whether immune activation and intestinal permeabilisation are more frequently observed in IBS patients compared with healthy controls. MethodsThe scientific bibliography was searched using the following keywords: irritable bowel syndrome, inflammation, immune activation, permeabilisation, intestine, assay, histology and human. The retrieved studies, including blood, faecal and histological studies, were analysed to provide a comprehensive and structured overview of the available data including the type of assay, type of inflammatory marker investigated or intestinal segment studied. ResultsImmune activation was more frequently observed in IBS patients than in healthy controls. An increase in the number of mast cells and lymphocytes, an alteration in cytokine levels and intestinal permeabilisation were reported in IBS patients. No consistent changes in the numbers of B cells or enterochromaffin cells or in mucosal serotonin production were demonstrated. ConclusionsThe changes observed were modest and often heterogeneous among the studied population. Only appropriate interventions improving irritable bowel syndrome symptoms could highlight and confirm the role of immune activation in this pathophysiology.

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Section: Intestinal Permeability In Ibs Patientsmentioning

confidence: 72%

Section: Intestinal Permeability In Ibs Patientsmentioning

confidence: 99%

Review article: associations between immune activation, intestinal permeability and the irritable bowel syndrome

Matricon

Méleine

Gelot

et al. 2012

Aliment Pharmacol Ther

183

180

View full text Add to dashboard Cite

show abstract

“…36,37 Whether similar mechanisms are implicated in the increased permeability during the developmental period at weaning need to be further investigated. Intestinal barrier disruption has been described to occur in IBS patients, [38][39][40][41] although the link between early adverse life events and increased intestinal permeability needs to be evaluated based on the present experimental data.…”

Section: Discussionmentioning

confidence: 99%

Chronic Early-life Stress in Rat Pups Alters Basal Corticosterone, Intestinal Permeability, and Fecal Microbiota at Weaning: Influence of Sex

Moussaoui

Jacobs

Larauche

et al. 2017

J Neurogastroenterol Motil

100

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Background/AimsWistar rat dams exposed to limited nesting stress (LNS) from post-natal days (PND) 2 to 10 display erratic maternal behavior, and their pups show delayed maturation of the hypothalamic-pituitary-adrenal axis and impaired epithelial barrier at PND10 and a visceral hypersensitivity at adulthood. Little is known about the impact of early life stress on the offspring before adulthood and the influence of sex. We investigated whether male and female rats previously exposed to LNS displays at weaning altered corticosterone, intestinal permeability, and microbiota. MethodsWistar rat dams and litters were maintained from PND2 to 10 with limited nesting/bedding materials and thereafter reverted to normal housing up to weaning (PND21). Control litters had normal housing. At weaning, we monitored body weight, corticosterone plasma levels (enzyme immunoassay), in vivo intestinal to colon permeability (fluorescein isothiocyanate-dextran 4 kDa) and fecal microbiota (DNA extraction and amplification of the V4 region of the 16S ribosomal RNA gene). ResultsAt weaning, LNS pups had hypercorticosteronemia and enhanced intestinal permeability with females > males while body weights were similar. LNS decreased fecal microbial diversity and induced a distinct composition characterized by increased abundance of Gram positive cocci and reduction of fiber-degrading, butyrate-producing, and mucus-resident microbes. ConclusionsThese data indicate that chronic exposure to LNS during the first week post-natally has sustained effects monitored at weaning including hypercorticosteronemia, a leaky gut, and dysbiosis. These alterations may impact on the susceptibility to develop visceral hypersensitivity in adult rats and have relevance to the development of irritable bowel syndrome in childhood.

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“…Due to increased intestinal permeability, exposure of submucosal tissue to antigens such as intraluminal pathogens occurs more frequently, overstimulating the local immune system. Various immune and inflammatory mediators activate intestinal receptors and cause disturbances in sensation, motility, and secretion in the digestive tract, contributing to the symptoms of IBS-D (Piche et al, 2009;Zhou et al, 2009). …”

Section: Mechanisms Of Ngf-mediated Barrier Dysfunctionmentioning

confidence: 99%

Nerve growth factor and diarrhea-predominant irritable bowel syndrome (IBS-D): a potential therapeutic target?

Liu

Yao

2016

J. Zhejiang Univ. Sci. B

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Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder characterized by recurrent abdominal pain or discomfort associated with abnormal bowel habits. Diarrhea-predominant IBS (IBS-D) is a major subtype of IBS, the predominant manifestations of which are abdominal pain and diarrhea. The pathogenesis of IBS-D remained unknown until recently. The effects of psychosocial stress, central hypervigilance, neuroendocrine abnormality, disturbed gastrointestinal motility, mucosal immune activation, intestinal barrier dysfunction, visceral hypersensitivity (VH), altered gut flora, and genetic susceptibility may be involved in its development. Recently, increased attention has been placed on the neural-immune-endocrine network mechanism in IBS-D, especially the role of various neuroendocrine mediators. As a member of the neurotrophin family, nerve growth factor (NGF) has diverse biological effects, and participates in the pathogenesis of many diseases. Basic studies have demonstrated that NGF is associated with inflammatory-and stress-related VH, as well as stress-related intestinal barrier dysfunction. The aim of this study is to summarize recent literature and discuss the role of NGF in the pathophysiology of IBS-D, especially in VH and intestinal barrier dysfunction, as well as its potential as a therapeutic target in IBS-D.

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Impaired intestinal barrier integrity in the colon of patients with irritable bowel syndrome: involvement of soluble mediators

Cited by 447 publications

References 32 publications

Review article: associations between immune activation, intestinal permeability and the irritable bowel syndrome

Review article: associations between immune activation, intestinal permeability and the irritable bowel syndrome

Chronic Early-life Stress in Rat Pups Alters Basal Corticosterone, Intestinal Permeability, and Fecal Microbiota at Weaning: Influence of Sex

Nerve growth factor and diarrhea-predominant irritable bowel syndrome (IBS-D): a potential therapeutic target?

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