Impaired immunomodulatory effects of seminal plasma may play a role in unexplained recurrent pregnancy loss: Results of an in vitro study

Fossé, NA du; Lashley, Eileen; Anholts, JDH; Beelen, Els van; Cessie, Saskia le; Lith, JMM van; Eikmans, Michael; Hoorn, MLP van der

doi:10.1016/j.jri.2022.103500

Cited by 5 publications

(17 citation statements)

References 34 publications

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“…Both studies used the same microbead‐based assay platform for IL16 quantification. IL16 concentration in SP was not associated with either HIV infection 36 or recurrent pregnancy loss 63 …”

Section: Resultsmentioning

confidence: 88%

“…SP IL6 was investigated in 64 studies published between 1994 and 2022 and comprising a total of 2966 normozoospermic and/or proven fertile men (Table 1). 5,6,8,10,11,14,15,17,19,22–24,26,35–37,39,42,43,45–47,49–53,55–60,63–68,77–100 Several methodologies have been utilised including ELISA (36 of 64 studies), multiplex microbead‐based assays (14 of 64 studies) and the Immulite 2000 automated chemiluminescence immunoassay analyser (seven of 64 studies) (Figure 2D). There was a moderate level of variance (CV = 93%) in reported concentrations of IL6, with median (range) = 15.9 (0.7–118) pg/mL.…”

Section: Resultsmentioning

confidence: 99%

“…There is evidence that CXCL8 is linked with male reproductive infection, 8,13–23 including men with recent COVID‐19 infection 26 and men with chronic prostatitis 24,25 . The abundance of CXCL8 in SP was investigated in a total of 1501 normozoospermic and/or proven fertile men in 40 studies published between 1993 and 2022 8,13–26,34,36,37,42,47,49,51,53,55–60,63–67,80,81,86,96,131 (Table 1). Five different methodologies were employed to measure CXCL8 concentration in SP, from diverse manufacturers.…”

Section: Resultsmentioning

confidence: 99%

“…IL16 concentration in SP was not associated with either HIV infection 36 or recurrent pregnancy loss. 63 IL17 is reported to be elevated in SP of infertile men, 75,101,108 although another study found no difference in men with various sperm abnormalities. 109 IL17 is reported to be elevated in SP from men with genital tract inflammation, 75 varicocoele, 109 chronic prostatitis, 25 Hepatitis B 108 and diabetes mellitus 110 compared to healthy normozoospermic men.…”

Section: Ilsmentioning

confidence: 90%

“…53,[55][56][57][58][59][60][63][64][65][66][67][68] Several methodologies have been utilised including ELISA (36 of 64 studies), multiplex microbead-based assays (14 of 64 studies) and the Immulite 2000 automated chemiluminescence immunoassay analyser (seven of 64 studies) (Figure2D). There was a moderate level of TA B L E 3…”

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confidence: 99%

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Immune regulatory cytokines in seminal plasma of healthy men: A scoping review and analysis of variance

et al. 2023

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ObjectiveSeminal plasma cytokines are associated with fertility and reproductive health, but progressing their clinical utility is hampered by absence of reference data on concentration ranges of relevant cytokines in healthy men. We employed a systematic approach to assemble current evidence on the concentrations of immune regulatory cytokines present in seminal plasma (SP) of normozoospermic and/or fertile men and evaluated the impact of different platform methodologies for cytokine quantification.Evidence reviewA systematic literature search was performed utilising PubMed, Web of Science and Scopus. Databases were searched from inception until 30th June 2022 inclusive, using combinations of keywords pertaining to seminal fluid and cytokines, and was restricted to human participants. Original data with values reported as concentration of specific cytokines in SP of men clearly defined as fertile or normozoospermic were extracted from studies written in English.ResultsA total of 3769 publications were initially identified, of which 118 fulfilled the eligibility criteria for inclusion. A total of 51 individual cytokines are detectable in SP of healthy men. The number of studies reporting on each cytokine range from 1 to >20. The reported concentrations for many cytokines linked with fertility status, including IL6, CXCL8/IL8, and TNFA, are highly variable between published studies. This is associated with the different immunoassay methodologies utilised and may be exacerbated by a lack of validation of assays to ensure suitability for SP assessment. Due to the large variation between studies, accurate reference ranges for healthy men cannot be determined from the published data.ConclusionsThe concentrations of cytokines and chemokines detected in SP is inconsistent and highly variable between studies and cohorts, limiting current capacity to define reference ranges for cytokine concentrations in fertile men. The lack of standardisation in methods used to process and store SP, and variation in platforms used to evaluate cytokine abundance, are factors contributing to the observed heterogeneity. To progress the clinical utility of SP cytokine analysis will require standardisation and validation of methodologies so that reference ranges for healthy fertile men can be defined.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%