Impaired fibrin gel permeability by high homocysteine levels

Quintana, Irene; Oberholzer, María Victoria; Kordich, L; Lauricella, Ana María

doi:10.1016/j.thromres.2009.08.013

Cited by 6 publications

(1 citation statement)

References 30 publications

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“…HHCY has been related to impaired formation of the fibrin network [ 36 , 37 ]. HCY impairs the fibrinolysis networks by inducing slower coagulation process and rendering more tightly packed fibrin clots [ 10 ].Under the influence of HCY, fibrin networks resulted in a more compact structure with shorter, thicker and more branched fibers, these structural properties of fibrin are related to slower spontaneous lysis rate of thrombus, and proved to be less permeable and more resistant to fibrinolysis [ 38 , 39 ]. Moreover, studies have revealed that mild HHCY (>10umol/L) showed markedly relationship with decreased tissue-type plasminogen activator (t-PA) activity (which is the major activator of fibrinolysis) and impaired spontaneous thrombolysis in STEMI patients [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Admission homocysteine is an independent predictor of spontaneous reperfusion and early infarct-related artery patency before primary percutaneous coronary intervention in ST-segment elevation myocardial infarction

Ying

Zhang³

et al. 2018

BMC Cardiovasc Disord

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BackgroundSpontaneous reperfusion (SR) and early infarct related artery (IRA) patency before primary percutaneous coronary intervention (PPCI) might bring extra benefit for patients with ST-segment elevation myocardial infarction (STEMI). This study premilinarily screened the independent predictors of SR, and assessed the relationship between SR and plasma homocysteine (HCY).MethodsThe medical records of 998 patients who were diagnosed as STEMI and underwent emergency coronary angiography were retrospectively studied, SR was defined as achievement of TIMI grade 3 flow in the IRA before PCI. The baseline characteristics, clinical manifestations and hematological variables were compared between SR and NSR group. Optimal cutoff point of HCY was calculated with receiving operating characteristics (ROC) analysis, multivariate logistic regression models were used to identify predictors of SR.Results229 (22.95%) patients showed angiographic SR. For HCY, the area under the curve was 0.70 (95% CI: 0.63–0.77, P = 0.034), the optimized cut off point was 17.55 μmol/L. Preinfarct angina (95% CI: 1.61–5.65, P = 0.0005), plasma C-reactive protein (CRP) level (95% CI: 0.87–0.99, P = 0.016) and HCY < 17.55 μmol/L (95% CI: 2.43–8.72, P < 0.0001) were found to be independent predictors for SR.ConclusionIn patients with STEMI, HCY < 17.55 μmol/L, preinfarct angina and plasma CRP level were independent predictors of SR.

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Section: Discussionmentioning

confidence: 99%

Admission homocysteine is an independent predictor of spontaneous reperfusion and early infarct-related artery patency before primary percutaneous coronary intervention in ST-segment elevation myocardial infarction

Ying

Zhang³

et al. 2018

BMC Cardiovasc Disord

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Impact of homocysteine-thiolactone on plasma fibrin networks

Genoud

Lauricella

Kordich

et al. 2014

J Thromb Thrombolysis

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Epidemiologic studies have shown that hyperhomocysteinemia is an independent risk factor for vascular disease. Homocysteine (Hcy) circulates as different species, mostly protein bound, and approximately 1% as its reduced form and the cyclic thioester homocysteine-thiolactone (HTL). Despite the level of plasma thiolactone being markedly low, detrimental effects are related to its high reactivity. HTL reacts with proteins by acylation of free basic amino groups; in particular, the epsilon-amino group of lysine residues forms adducts and induces structural and functional changes in plasma proteins. In order to assess the effects of HTL on plasma fibrin networks, a pool of normal plasma incubated with HTL (100, 500 and 1,000 μmol/L, respectively) was evaluated by global coagulation tests and fibrin formation kinetic assays, and the resulting fibrin was observed by scanning electron microscopy. HTL significantly prolonged global coagulation tests in a concentration-dependent manner with respect to control, and increases were up to 14.5%. Fibrin formation kinetic parameters displayed statistically significant differences between HTL-treated plasma and control in a concentration-dependent way, showing higher lag phase and lower maximum reaction velocity and final network optical density. Electron microscopy analysis of HTL plasma networks revealed a compact architecture, with more branches and shorter fibers than control. We can conclude that HTL induced a slower coagulation process, rendering more tightly packed fibrin clots. Since these features of the networks have been related to impaired fibrinolysis, the N-homocysteinylation reactions would be involved in the prothrombotic effects associated to hyperhomocysteinemia.

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Hydrogen sulfide in hemostasis: Friend or foe?

Olas

2014

Chemico-Biological Interactions

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Impaired fibrin gel permeability by high homocysteine levels

Cited by 6 publications

References 30 publications

Admission homocysteine is an independent predictor of spontaneous reperfusion and early infarct-related artery patency before primary percutaneous coronary intervention in ST-segment elevation myocardial infarction

Admission homocysteine is an independent predictor of spontaneous reperfusion and early infarct-related artery patency before primary percutaneous coronary intervention in ST-segment elevation myocardial infarction

Impact of homocysteine-thiolactone on plasma fibrin networks

Hydrogen sulfide in hemostasis: Friend or foe?

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