Impacts of two perfluorinated compounds (PFOS and PFOA) on human hepatoma cells: Cytotoxicity but no genotoxicity?

Enhanced cytotoxicity of pentachlorophenol by perfluorooctane sulfonate or perfluorooctanoic acid in HepG2 cells

“…1A illustrates the viability of the cells which were exposed to PFOS or PFOA solution alone for 3 h. The cell viability was not affected by PFOA at all the tested concentrations (100, 200, 300 and 400 lM), but was slightly inhibited by PFOS at 300 and 400 lM. In the other words, both PFOS and PFOA with a concentration lower than 200 lM did not affect the cell viability in 3 h. The results agree with the study of Florentin et al, who conducted MTT assay for 1 h and 24 h (Florentin et al, 2011). But Hu and Hu reported that exposure to 150 lM PFOS for 24 h could cause a decrease of cell viability by 8%, while treatment with 100 lM PFOS or PFOA for 24 h did not show significant impacts on the cell viability (Hu and Hu, 2009).…”

NTP Technical Report on the Toxicity Studies of Perfluoroalkyl Sulfonates (Perfluorobutane Sulfonic Acid, Perfluorohexane Sulfonate Potassium Salt, and Perfluorooctane Sulfonic Acid) Administered by Gavage to Sprague Dawley (Hsd:Sprague Dawley SD) Rats

“…Significant increases in DNA damage were detected using the comet assay in HepG2 cells exposed to PFOS at concentrations ranging from 0.2 to 20 μM for 24 hours 74 . However, negative comet assay results were reported in HepG2 cells exposed to PFOS at concentrations ranging from 5 to 300 μM for 1 or 24 hours 75 . Furthermore, no induction of micronuclei was observed in HepG2 cells exposed to the same concentrations of PFOS that were used for the 24-hour comet assay 75 .…”

Human dietary exposure to perfluoroalkyl substances in Catalonia, Spain. Temporal trend

“…In recent years, a number of studies involving PFASs have focused on increasing general knowledge on their toxicity (Florentin, Deblonde, Diguio, Hautemaniere, & Hartemann, 2011;Fuentes, Colomina, Vicens, Franco-Pons, & Domingo, 2007;Johansson, Eriksson, & Viberg, 2009;Ribes, Fuentes, Torrente, Colomina, & Domingo, 2010), environmental distribution and fate (Houde et al, 2011;Martin, Whittle, Muir, & Mabury, 2004;Nakata et al, 2006;Yamashita et al, 2005), as well as potential human health risks of exposure to these pollutants, especially to PFOS and PFOA (Cornelis et al, 2012;Domingo, 2012;Egeghy & Lorber, 2011;Haug, Huber, Becher, & Thomsen, 2011;Liu et al, 2010;Zhao et al, 2011). However, significant gaps still exist on that knowledge.…”