Impacts of resveratrol versus platelet-rich plasma for treatment of experimentally lithium-induced thyroid follicular cell toxicity in rats. A histological and immunohistochemical study

El-Mahalaway, Abeer M.; El-Azab, Nahla El-Eraky

doi:10.1080/01913123.2019.1593270

Cited by 7 publications

(11 citation statements)

References 30 publications

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“…It is caused as a result of the increased demand for blood to feed the follicular cells or as a result of the increased thyroid gland's size (El-Mahalaway and El-Azab, 2019). Furthermore, congestion can be part of the previously stated inflammatory process that accompanies LC toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…LC was obtained in the structure of tablets (400 mg) (Nile Co. for Pharmaceuticals and Chemical Industries, Egypt). Tablets were liquified in distilled water and given through an intragastric tube in a dose of 30 mg/kg/day for eight weeks (El-Mahalaway and El-Azab, 2019). GTE was obtained in the structure of tablets (200 mg) (Techno-med Group Co., Egypt).…”

Section: Test Materialsmentioning

confidence: 99%

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Green tea extract modulates lithium-induced thyroid follicular cell damage in rats

et al. 2022

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Background:The aim of the current work was to clarify the modulation role of green tea extract (GTE) over structural and functional affection of the thyroid gland after long term use of lithium carbonate (LC). The suggested underlying mechanisms participating in thyroid affection were researched.Materials and methods: Twenty-four Sprague-Dawley adult albino rats were included in the work. They are divided into three groups (control, LC, and concomitant LC + GTE). The work was sustained for 8 weeks. Biochemical assays were achieved (thyroid hormone profile, IL-6).Histological, histochemical (PAS) and immunohistochemical (caspase-3, TNF-α, PCNA) evaluations were done. Oxidative/antioxidative markers (MDA / GSH, SOD) and western blot evaluation of the Bcl2 family were done.Results: LC induced hypothyroidism (decrease T3, T4/increase TSH). The follicles were distended, others were involuted. Some follicles were disorganized, others showed detached follicular cells. Apoptotic follicular cells were proved (Bax and caspase-3 increased, Bcl2 decreased, Bax/Bcl2 ratio increased). The collagen fibers' content and proinflammatory markers (TNF-α and IL-6) increased. The proliferative nuclear activity was supported by increase 2 expression of PCNA. Oxidative stress was established (increase MDA/decrease GSH, SOD).With the use of GTE, the thyroid hormone levels increased, while the TSH level decreased.Apoptosis is improved as Bax decreased, Bcl2 increased, and Bax/Bcl2 ratio was normal. The collagen fibers' content and proinflammatory markers (TNF-α and IL-6) decreased. The expression of PCNA and caspase-3 were comparable to the control group. The oxidative markers were improved (decrease MDA/increase GSH, SOD). Conclusions:In conclusion, prolonged use of LC results in hypothyroidism, which is accompanied by structural thyroid damage. LC induced thyroid damage through oxidative stress that prompted sterile inflammation and apoptosis. With the use of GTE, the thyroid gland achieved its structure and function. The protecting role of GTE is through antioxidant, antifibrotic, anti-inflammatory, and antiproliferative effects.

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Section: Discussionmentioning

confidence: 99%

Section: Test Materialsmentioning

confidence: 99%

Green tea extract modulates lithium-induced thyroid follicular cell damage in rats

et al. 2022

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“…Other researches [11,12,44] attributed the ability of PRP to accelerate the epithelial cell regeneration to the biological effects of many growth factors and different cytokines as platelet derived growth factor, vascular endothelial growth factor and transforming growth factor of platelets. These growth factor leads to cell proliferation of epithelial cells, promote tissue angiogenesis, increase blood flow, and improve oxygenation in the site of injury.…”

Section: Discussionmentioning

confidence: 99%

“…Different researches [9,11,13,44] found that PRP has an antifibrotic effect as it contains antifibrotic molecules, growth factors, and serum amyloid protein which could ameliorate the fibrosis. Additionally, PRP treatment increases muscle regeneration by stimulating myogenesis [47,48] .…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the impact of platelet rich plasma and hyaluronic acid on ketamine-induced cystitis in albino rats : A histological and immunohistochemical study.

El-Azab

El-Mahalaway

Mohamed

2019

Egyptian Journal of Histology

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Background: ketamine induced cystitis (KIC) is commonly occurred in ketamine abusing individuals. It is associated with damage in the lining of the bladder and lower urinary tract symptoms. Platelets rich plasma (PRP) is attractive therapeutic line in regenerative medicine. Hyaluronic acid (HA) has many beneficial therapeutic effects in treatment of different types of cystitis. Objective: to evaluate the effect of PRP and HA on ketamine induced cystitis in adult male rats. Materials and Methods: Forty-four adult male rats were divided into five groups: Group I: control rats. Group II: Ketamine group. Group III: Recovery group. Group IV: Ketamine and PRP treated group. Group V: Ketamine and HA treated group. Specimens from the body of the urinary bladder were processed and examined histologically and immunohistochemically. Morphometrical studies and statistical analysis were conducted. Results: Groups II and III showed focal areas of degeneration and ulceration with apparently decreased thickness of the urothelium. Cellular infiltration and dilated congested blood vessels of the lamina propria were also observed. A significant increase of the collagen fibers in the lamina propria and between the muscle bundles. Nuclear factor kappa B (NF-κB) immunoreaction was significantly increased but there was a non-significant increase of antiproliferating cell nuclear antigen (PCNA) immunopositive nuclei among urothelium and a significant decrease of desmin, compared to group I. Groups III and IV showed improvement of most of the histological and immunohistochemical changes described before. Conclusion:The intravesical injection of PRP and HA provide a positive impact on treatment of KIC. HA therapy is a more efficient mean as it provides a better improvement in healing of urothelium and promotes more rapid tissue regeneration.

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Pomegranate (Punica granatum) peel alleviates lithium‐induced alterations in the thyroid gland of rats by modulating apoptosis and oxidative stress

El-Din

Fadel

Rashid

et al. 2022

The Anatomical Record

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Lithium carbonate (LC) is known to alter thyroid gland function. Pomegranate (PG) is a fruit with multiple antioxidant and antiapoptotic properties. Here, we studied the effect of PG on LC-induced morphological and functional alterations in the thyroid glands of rats. Rats were divided into four groups: control, lithium, lithium-PG, and PG. After 8 weeks, the rats were sacrificed, the levels of thyroid hormones and oxidative stress markers were estimated, and thyroid tissues were subjected to histological, immunohistochemical, and ultrastructural evaluations. Compared to the control group, the lithium group showed significant changes in thyroid hormone levels, greater expression of the oxidant marker malondialdehyde, and lower expression of the antioxidant marker superoxide dismutase (SOD). Most of these changes improved upon PG treatment. Histological evaluation of the thyroid in the lithium group showed disorganization and follicle involution. Additionally, the periodic acid Schiff staining intensity and SOD immunoreactivity declined significantly, whereas the collagen fiber content and Bax immunoreactivity increased. The follicular ultrastructure showed marked distortion. These changes were mitigated upon PG treatment. In conclusion, PG alleviated the morphological and functional changes in the thyroid glands induced by LC by modulating apoptosis and oxidative stress.

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Impacts of resveratrol versus platelet-rich plasma for treatment of experimentally lithium-induced thyroid follicular cell toxicity in rats. A histological and immunohistochemical study

Cited by 7 publications

References 30 publications

Green tea extract modulates lithium-induced thyroid follicular cell damage in rats

Green tea extract modulates lithium-induced thyroid follicular cell damage in rats

Evaluation of the impact of platelet rich plasma and hyaluronic acid on ketamine-induced cystitis in albino rats : A histological and immunohistochemical study.

Pomegranate (Punica granatum) peel alleviates lithium‐induced alterations in the thyroid gland of rats by modulating apoptosis and oxidative stress

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