Impacts of life history and biogeography on the genetic stock structure of Atlantic sturgeon Acipenser oxyrinchus oxyrinchus, Gulf sturgeon A. oxyrinchus desotoi, and shortnose sturgeon A. brevirostrum

Conservation of Atlantic sturgeon Acipenser oxyrinchus oxyrinchus: delineation of stock structure and distinct population segments

“…1) were analyzed. Mitochondrial DNA data on a subset of these specimens were reported in Wirgin et al (2000) and Waldman et al (2002) while others were either newly acquired or newly characterized. New specimens (n = 374) that were analyzed for this study include 25 from the St. Lawrence, 56 from the Saint John, 30 from the Hudson, 19 from the Delaware, 40 from the James, 5 from the Edisto, 24 from the Savannah, 44 from the Ogeechee, and 131 from the Altamaha Rivers.…”

“…PCR products of samples that were characterized previously were sequenced manually as described in (Wirgin et al 2000;Waldman et al 2002). Amplicons from specimens newly characterized in this study were Dye-Terminator Cycle Sequenced (DTCS) as described by the manufacturer (Beckman Coulter, Inc., Fullerton, CA).…”

“…Evidence of significant geographic discontinuities in both mitochondrial DNA (mtDNA) haplotype (Wirgin et al 2000;Waldman et al 2002) and microsatellite allelic frequencies (King et al 2001) among Atlantic sturgeon populations suggests they are reproductively isolated and exhibit high philopatry to natal estuaries. Maternally derived gene flow estimates generally were less than 1 migrant/generation, supporting the notion that straying among riverine populations was low.…”

Kemp's Ridley Sea Turtle Saga and Setback: Novel Analyses of Cumulative Hatchlings Released and Time-Lagged Annual Nests in Tamaulipas, Mexico

“…The loss of any population within a DPS will result in (1) a long-term gap in the range of the DPS that is unlikely to be recolonized, (2) loss of reproducing individuals, (3) loss of genetic biodiversity, (4) potential loss of unique haplotypes, (5) potential loss of adaptive traits, (6) reduction in total number, and 7) potential for loss of population source of recruits. The loss of a population will negatively impact the persistence and viability of the DPS as a whole, as fewer than two individuals per generation spawn outside their natal rivers (King et al 2001;Waldman et al 2002;Wirgin et al 2000). The persistence of individual populations, and in turn the DPS, depends on successful spawning and rearing within the freshwater habitat, the immigration into marine habitats to grow, and then the return of adults to natal rivers to spawn.…”

Low Danube Sturgeon Identification Using DNA Markers

“…These markers were initially used as a focus in two directions: in order to establish the genetic diversity of sturgeon populations with a distinct geographic distribution (Miracle & Campton, 1995;Szalanski et al, 2001;Waldman et al, 2002) and in order to establish the usefulness of these markers for recovering the phylogeny of the Acipenseridae class (Brown et al, 1996). In recent years PCR-RFLP was proposed as a molecular method for the identification of sturgeon species and implicitly for the determination of the origin of the caviar on the market.…”