Impacts of Blended Bombyx mori Silk Fibroin and Recombinant Spider Silk Fibroin Hydrogels on Cell Growth

Abstract

Binary-blended hydrogels fabricated from Bombyx mori silk fibroin (SF) and recombinant spider silk protein eADF4(C16) were developed and investigated concerning gelation and cellular interactions in vitro. With an increasing concentration of eADF4(C16), the gelation time of SF was shortened from typically one week to less than 48 h depending on the blending ratio. The biological tests with primary cells and two cell lines revealed that the cells cannot adhere and preferably formed cell aggregates on eADF4(C16) hydrogels, due to the polyanionic properties of eADF4(C16). Mixing SF in the blends ameliorated the cellular activities, as the proliferation of L929 fibroblasts and SaOS-2 osteoblast-like cells increased with an increase of SF content. The blended SF:eADF4(C16) hydrogels attained the advantages as well as overcame the limitations of each individual material, underlining the utilization of the hydrogels in several biomedical applications.

Degradable Elastomeric Silk Biomaterial for Flexible Bioelectronics

“…For instance, SF hydrogels were shown to support L929 proliferation, but at a slower rate than TCP. 69 Higher SF content correlates to higher rates of fibroblast proliferation due to the presence of growth-promoting polypeptides in the N-terminal region of the fibroin polypeptide. Fibroblasts on SF-glycerol films suggested that less-favorable, early-stage fibroblast adhesion and growth stems from silk's hydrophobic domains and lack of surface topography.…”

“…Substrates supported cell proliferation, with cell number logarithmically increasing over 7 days, in agreement with previous findings. For instance, SF hydrogels were shown to support L929 proliferation, but at a slower rate than TCP . Higher SF content correlates to higher rates of fibroblast proliferation due to the presence of growth-promoting polypeptides in the N-terminal region of the fibroin polypeptide.…”

A comprehensive review of silk-fibroin hydrogels for cell and drug delivery applications in tissue engineering and regenerative medicine

“…The study also confirmed calcium production through alizarin red staining. Importantly, this study reveals that hydrogels can carry drugs and cells efficiently with their functionalities intact [173] .…”

Evaluation of Injectable Composite Material Comprising Biphasic Bone Substitutes and Crosslinked Collagen

“…[ 11 ] The ability of fulling irregular shaped defects makes injectable materials can conform to the shape and size of the bone defect, providing a customized fit that can improve the effectiveness of the treatment. [ 5 ] Moreover, they can promote the recruitment and proliferation of bone‐forming cells, [ 12 ] accelerate the formation of new bone tissue, and enhance the integration of the implant with the surrounding bone. [ 13 ]…”