Impacts of an Orange Oil Solvent and Stickem® on the Detection of<i>Xylella fastidiosa</i>DNA in Glassy-Winged Sharpshooters,<i>Homalodisca vitripennis</i>(Hemiptera: Cicadellidae)

Marshall, Patrick; Hail, Daymon; Mitchell, Forrest L.; Bextine, Blake

doi:10.1653/024.093.0309

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…Sharpshooters were removed from the sticky traps using orange oil (Citrus King, www.citrusdepot.net) and sterile tweezers, immediately surface sterilized by submerging in 10 % bleach followed by 95 % ethanol, then washed with sterile DI water (Marshall et al 2010). The abdomen and thorax of each insect were immediately removed from the insect and stored together at −20°C in a clean, sterile 1.5 μl microcentrifuge tube.…”

Section: H Vitripennis Genomic Dna Extractionsmentioning

confidence: 99%

Geographic patterns in the bacterial microbiome of the glassy-winged sharpshooter, Homalodisca vitripennis (Hemiptera: Cicadellidae)

2015

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Numerous environmental and physiological factors influence the composition of the bacterial microbiome inhabiting an insect. Pyrosequencing of bacterial 16S rRNA sequences was used to identify bacterial taxa inhabiting 50 Homalodisca vitripennis collected from nine Texas vineyards collected during the summer of 2007. After quantifying distances between insects based on differences in bacterial community composition, it was clear that the geographical locations of the insects collected had a substantial influence on their internal bacterial microbiota composition. There was a clear tendency for insects collected from the same vineyard to cluster in principal component analyses using said distances. A single bacterial species, Candidatus Baumannia cicadellinicola, a primary endosymbiont, represented the majority of bacteria sampled. Many of the more common bacterial genera contain a majority of species known previously to inhabit soil and plants exclusively, suggesting that the insect's environment is a major source of abundant species of microflora. Many of the bacterial genera sampled had significant positive correlations with other bacteria, and many of these correlations were between genera with the same order.

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Section: H Vitripennis Genomic Dna Extractionsmentioning

confidence: 99%

Geographic patterns in the bacterial microbiome of the glassy-winged sharpshooter, Homalodisca vitripennis (Hemiptera: Cicadellidae)

2015

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“…However, some sampling procedures such as the use of sticky traps can affect sample integrity for molecular detection (Marshall et al . ), and optimisation of DNA extraction protocols is necessary when dealing with the small numbers of bacterial cells found in insect mouthparts (Bextine et al . ).…”

Section: Assessing Vector Abundance and Diversitymentioning

confidence: 99%

Sharpshooters: a review of what moves Xylella fastidiosa

et al. 2019

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Sharpshooters (Hemiptera: Cicadellidae: Cicadellinae) and spittlebugs (Hemiptera: Aphrophoridae: Aphrophorinae) have a worldwide distribution and are often associated with many crops. Because the geographic range of sharpshooters and spittlebugs often overlaps with the range of the plant pathogenic bacterium Xylella fastidiosa, the importance of these groups of insects in spreading X. fastidiosa rises to the level of a major socioeconomic problem. Managing diseases caused by X. fastidiosa is challenging for several reasons. First, multiple sequence types of the pathogen have been identified, with sequence type affecting host plant specificity. Second, multiple vector species may be present in an agroecosystem, and the host range of vector species is often broad. Although multiple vector control options such as biological, chemical, physical and cultural controls have been described, disease control programs must take into account biological and ecological parameters of locally dominant X. fastidiosa sequence types and vector species to develop suitable control strategies. Our review demonstrates how fundamental principles of vector reproductive biology, behaviour, nutrition and population and community ecology have influenced research on insect vectors and provides insights into priority research areas.

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High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples

Brady¹,

Faske²,

Castañeda-Gill

et al. 2011

Journal of Microbiological Methods

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Impacts of an Orange Oil Solvent and Stickem® on the Detection ofXylella fastidiosaDNA in Glassy-Winged Sharpshooters,Homalodisca vitripennis(Hemiptera: Cicadellidae)

Cited by 4 publications

References 10 publications

Geographic patterns in the bacterial microbiome of the glassy-winged sharpshooter, Homalodisca vitripennis (Hemiptera: Cicadellidae)

Geographic patterns in the bacterial microbiome of the glassy-winged sharpshooter, Homalodisca vitripennis (Hemiptera: Cicadellidae)

Sharpshooters: a review of what moves Xylella fastidiosa

High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples

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