Impacto prognóstico da expressão imuno-histoquímica do BRCA1 nos carcinomas mamários esporádicos

Ribeiro‐Silva, Alfredo; Garcia, Sérgio Britto; Chahud, Fernando; Zucoloto, Sérgio

doi:10.1590/s1676-24442005000300010

Cited by 6 publications

(7 citation statements)

References 19 publications

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“…Carcinomas that exhibit reduced expression of BRCA1 are typically poorly differentiated with high proliferative rate, and inversely correlated with histologic grade [ 49 ]. In dogs, the study of the expression of the BRCA1 gene demonstrated that loss of BRCA1 expression was associated with increased Ki67 proliferation marker and the absence of estrogen receptor (ER) and other malignancy [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

“…Although hereditary mutations in BRCA1 gene are associated with familial breast cancer, the majority of the mammary carcinomas present sporadic occurrence. Although mutations in the BRCA1 are rare in non-familial mammary carcinomas, it is common to have loss of expression in 40 to 50% of sporadic mammary tumors, possibly through epigenetic alterations, specifically promoter hypermethylation [ 49 ]. In this study, the results of qPCR indicated that the highest levels of BRCA1 are presented in a complex carcinoma (CCa1), while cultured cells obtained from a complex adenoma showed the highest levels of BRCA2, followed by complex carcinomas.…”

Section: Discussionmentioning

confidence: 99%

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Establishment of primary mixed cell cultures from spontaneous canine mammary tumors: Characterization of classic and new cancer-associated molecules

et al. 2017

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There are many factors which make canine cancer like cancer in humans. The occurrence of spontaneous mammary tumors in pet dogs, tumor genetics, molecular targets and exposure to the same environmental risk factors are among these factors. Therefore, the study of canine cancer can provide useful information to the oncology field. This study aimed to establish and characterize a panel of primary mixed cell cultures obtained from spontaneous canine mammary tumors. Eight established cell cultures obtained from one normal mammary gland, one complex adenoma, one mixed adenoma, two complex carcinomas and two mixed carcinomas were analyzed. The gene expression levels of classic molecular cancer players such as fibroblast growth factor receptor (FGFR) 2, breast cancer (BRCA) 1, BRCA2 and estrogen receptor (ESR) 1 were evaluated. For the first time, three orphan nuclear receptors, estrogen-related receptors (ERRs) α, β and γ were studied in canine mammary cancer. The highest expression level of ERRα was observed in complex carcinoma-derived cell culture, while the highest levels of ERRβ and γ were observed in cells derived from a mixed carcinoma. Meanwhile, complex carcinomas presented the highest levels of expression of ESR1, BRCA1 and FGFR2 among all samples. BRCA2 was found exclusively in complex adenoma. The transcription factor GATA3 had its highest levels in mixed carcinoma samples and its lowest levels in complex adenoma. Proliferation assays were also performed to evaluate the mixed cell cultures response to ER ligands, genistein and DES, both in normoxia and hypoxic conditions. Our results demonstrate that morphological and functional studies of primary mixed cell cultures derived from spontaneous canine mammary tumors are possible and provide valuable tool for the study of various stages of mammary cancer development.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Establishment of primary mixed cell cultures from spontaneous canine mammary tumors: Characterization of classic and new cancer-associated molecules

et al. 2017

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“…Groups labeling of each prognostic factor was based on previous works described in the literature. [34] To further evaluate the correlations between trace elements distribution on the images obtained by m-XRF, maps of individual elements were combined by providing correlation maps. To assess the statistical significance of the correlations between images, we performed the nonparametric Spearman test.…”

Section: Discussionmentioning

confidence: 99%

Role of Ca, Fe, Cu and Zn in breast cancer: study by X‐ray fluorescence techniques and immunohistochemical analysis

Silva¹,

Silva²,

Conceição³

et al. 2013

X-Ray Spectrometry

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a In recent years, several studies have shown that concentrations of trace elements are altered in neoplastic breast tissues. However, the microenvironment and metabolic changes caused by tumors are complex and still not completely understood. Under this aspect, the combination of different techniques to investigate the role of trace elements in promoting and/or maintaining a tumor is interesting once the combination of information obtained by analytical techniques and immunohistochemical assays, associated with clinicopathological data, may allow a better metabolic understanding of trace elements in breast cancer.In this work, the role of the trace elements Ca, Fe, Cu and Zn in neoplastic breast tissues was investigated by X-ray fluorescence (XRF) techniques and immunohistochemical assays. We determined concentrations of Ca, Fe, Cu and Zn in normal and neoplastic breast tissues using energy dispersive XRF, and these values were used to set the positive or negative expression of elements in normal and neoplastic tissues. These expressions were correlated with the spatial distributions of trace elements (evaluated by micro-XRF) and with immunoexpression of matrix metalloproteinases (MMPs), tissue inhibitors of MMPs and vascular endothelial growth factor.The results revealed that the expression of the trace elements Fe, Cu and vascular endothelial growth factor are related, indicating that higher levels of these elements can be associated with the angiogenic process in breast cancer. Also, associations between Ca, Zn and MMPs expression have been observed, possibly because of the fact that both metals are present in these proteins.

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“…No entanto, a perda da expressão do RNA mensageiro e da proteína codificada por este gene ocorre em 40 a 50% dos tumores mamários esporádicos, possivelmente por alterações epigenéticas, particularmente a hipermetilação. Os carcinomas que apresentam expressão reduzida de BRCA1 tipicamente são pouco diferenciados, com alta taxa proliferativa e correlação inversa com o grau histológico (RIBEIRO-SILVA et al, 2005).…”

Section: Revisão De Literaturaunclassified

“…A epigenética explicaria, também, o fato que, embora raras as mutações no gene supressor de tumor BRCA1 nos carcinomas mamários esporádicos na mulher, a perda da expressão da proteína por ele codificada está freqüentemente presente nas neoplasias mamárias nãofamiliares. O silenciamento deste gene por mecanismos epigenéticos como a hipermetilação pode ser a explicação para este fenômeno (RIBEIRO-SILVA et al, 2005;JOVANOVIC et al, 2010).…”

Section: Dos Estudos Dos Receptores Relacionados Ao Receptor De Estróunclassified

Neoplasias mamárias caninas: alterações genéticas e epigenéticas e sua correlação com tipo histológico e prognóstico

Biondi¹

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Impacto prognóstico da expressão imuno-histoquímica do BRCA1 nos carcinomas mamários esporádicos

Cited by 6 publications

References 19 publications

Establishment of primary mixed cell cultures from spontaneous canine mammary tumors: Characterization of classic and new cancer-associated molecules

Establishment of primary mixed cell cultures from spontaneous canine mammary tumors: Characterization of classic and new cancer-associated molecules

Role of Ca, Fe, Cu and Zn in breast cancer: study by X‐ray fluorescence techniques and immunohistochemical analysis

Neoplasias mamárias caninas: alterações genéticas e epigenéticas e sua correlação com tipo histológico e prognóstico

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