Impact of Zinc Orthophosphate on Simulated Drinking Water Biofilms Influenced by Lead and Copper

Payne, Sarah Jane; Piorkowski, Gregory S.; Hansen, Lisbeth Truelstrup; Gagnon, Graham A.

doi:10.1061/(asce)ee.1943-7870.0001031

Cited by 11 publications

(12 citation statements)

References 39 publications

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“…However, the increase in phosphate tended to reduce bacterial richness, whilst promoted bacterial dominance, thus fewer OTUs were predominant in the community. This finding contradicts those of Payne et al (2016), who observed an increase in size and diversity of biofilm communities as a result of phosphate treatment in galvanic macrocells with lead and copper components fed with drinking water. Similarly, Jang et al (2012), reported an increase of species diversity in biofilm when phosphate was added under low residual chlorine conditions in an annular reactor also fed with drinking water.…”

Section: Effect Of Phosphate On the Biofilm Taxonomic Analysiscontrasting

confidence: 99%

“…In addition, those studies focused on investigating bacterial communities, yielded contradictory results about the effect of phosphate on these microorganisms. On the one hand, several researchers found that phosphate favoured bacterial growth (Miettinen et al, 1997;Sathasivan et al, 1997), increased bacterial cell numbers (Kasahara et al, 2004;Fang et al, 2010), promoted biofilm viability (Rubulis and Juhna, 2007) and increased Gram-negative bacterial content (Kasahara et al, 2004) and diversity (Jang et al, 2012;Payne et al, 2016). On the other hand, other researchers indicated that phosphate did not affect bacterial density (Berger et al, 1992;Gouider et al, 2009) and a decrease of bacteria was observed when this chemical was added (Appenzeller et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Phosphate Dosing in Drinking Water Distribution Systems Promotes Changes in Biofilm Structure and Functional Genetic Diversity

et al. 2020

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Water utilities treat drinking water by adding phosphate to prevent metal dissolution from water pipe work systems and particularly lead poisoning. Phosphate can be a limiting nutrient for microbial biofilms in DWDS, yet its effects on these microbial consortia are not well understood. This research presents results from phosphate dosing experiments using a real scale chlorinated DWDS, comparing standard phosphate concentrations of United Kingdom drinking water (1 mgP/L) with a double dose (2 mgP/L) commonly used in plumbosolvency treatment. Biofilm development during phosphate treatment experiments was monitored using a holistic approach by combining metagenomics analysis, flow cytometry and SEM characterisation. The increase of phosphate levels in drinking water, reduced biofilm cell numbers and promoted the presence of poorly distributed biofilms on inner pipe surfaces. Metagenomics analysis using genetic markers (16S rRNA and ITS2) showed that phosphate influenced biofilm community structure, particularly fungal composition. Whole metagenome sequencing showed that phosphate enrichment favoured the presence of sequencing reads associated to ATPases, ion transporters and DNA-interacting proteins, whilst reads associated to nitrogen metabolism were predominant in control samples. This research brings new knowledge regarding the influence of phosphate treatment on the composition and structure of biofilms within DWDS, and the implications that this might have for the management of these systems.

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Section: Effect Of Phosphate On the Biofilm Taxonomic Analysiscontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phosphate Dosing in Drinking Water Distribution Systems Promotes Changes in Biofilm Structure and Functional Genetic Diversity

et al. 2020

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“…The increase in biomass concentrations in the orthophosphate-treated system is consistent with previous drinking water studies reporting an increase in microbial growth due to orthophosphate. [6][7][8]10,46,47 Lehtola et al 7 reported an increase in biomass concentrations after adding orthophosphate as little as 1 µg P-PO4 3-L -1 to treated drinking water with no disinfectant residual and a background of 0.19 µg L -1 of available phosphorus. While Rompré et al 2 and Kogo et al 19 indicated that biofilm accumulation was not influenced by corrosion inhibitor type (sodium silicate or orthophosphate), the results from this study suggest the opposite: tATP concentrations were significantly lower in the silicate-treated AR compared to the orthophosphate-treated AR.…”

Section: Cellular Atp (Catp) and Biofilm Atp (Tatp)mentioning

confidence: 99%

Effect of sodium silicate on drinking water biofilm development

Munoz

Trueman

et al. 2021

Preprint

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Sodium silicates have been studied for sequestration of iron, coagulation, and corrosion control, but their impact on biofilm formation has not been documented in detail. This study investigated the impact of sodium silicate corrosion control on biomass accumulation in drinking water systems in comparison to orthophosphate, a common corrosion inhibitor. Biofilm growth was measured by determining ATP concentrations, and the bacterial community was characterized using 16S ribosomal RNA (rRNA) sequencing. A pilot-scale study with cast-iron pipe loops, annular reactors (ARs), and polycarbonate coupons demonstrated significantly lower biofilm ATP concentrations in the sodium silicate-treated AR than the orthophosphate-treated AR when the water temperature exceeded 20°C. However, an elevated sodium silicate dose (48 mg L-1 of SiO2) disturbed and dispersed the biofilm formed inside the AR, resulting in elevated effluent ATP concentrations. Two separate experiments confirmed that biomass accumulation was higher in the presence of orthophosphate at high water temperatures (20°C) only. No significant differences were identified in biofilm ATP concentrations at lower water temperatures (below 20°C). Differences in bacterial communities between the orthophosphate- and sodium silicate-treated systems were not statistically significant, even though orthophosphate promoted higher biofilm growth. However, the genera Halomonas and Mycobacterium—which include opportunistic pathogens—were present at greater relative abundances in the orthophosphate-treated system compared to the sodium silicate system.

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“…In relation to lead, there are only few studies focusing on the study of microbial communities growing on this type of metal [15][16][17]20,25 and results from these suggested that lead did not affect biofilm biomass 20 , richness 20 and bacterial numbers 17 , yet exerted a selective pressure on biofilm communities by favouring bacteria able to accumulate and resist heavy metals 15,17,20 .…”

Section: Introductionmentioning

confidence: 99%

“…Several studies conclude that PO 4 3− promoted the development of planktonic microorganisms in drinking water, and changed the bacterial community structure increasing Gram-negative bacterial content 18 . In relation to biofilm development on a range of substrates, it has been reported that PO 4 3− increased the number of bacterial cells, promoted biofilm viability 19 and diversity 20 , 21 , but reduced the production of exopolysaccharides 22 . In contrast, other studies indicated that the bacterial density was not affected by PO 4 3− 23 and even a decrease of heterotrophic bacteria was observed 24 .…”

Section: Introductionmentioning

confidence: 99%

Influence of phosphate dosing on biofilms development on lead in chlorinated drinking water bioreactors

Olmo

Arslan

Jensen

et al. 2020

npj Biofilms Microbiomes

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Phosphate dosing is used by water utilities to prevent plumbosolvency in water supply networks. However, there is a lack of knowledge regarding biofilm formation on lead and plastic materials when phosphate concentrations are modified in drinking water systems. In this study, biofilms were grown over lead coupons and PVC tubes in bioreactors supplied with local drinking water treated to provide different phosphate doses (below 1, 1 and 2 mg/L) over a period of 28 days. A range of commercial iron pellets (GEH104 and WARP) were tested aiming to maintain phosphate levels below the average 1 mg/L found in drinking water. Changes in biofilm community structure in response to three different phosphate treatments were characterised by Illumina sequencing of the 16S rRNA gene for bacteria and the ITS2 gene for fungi. Scanning electron microscopy was used to visualise physical differences in biofilm development in two types of materials, lead and PVC. The experimental results from the kinetics of phosphate absorption showed that the GEH104 pellets were the best option to, in the long term, reduce phosphate levels while preventing undesirable turbidity increases in drinking water. Phosphate-enrichment promoted a reduction of bacterial diversity but increased that of fungi in biofilms. Overall, higher phosphate levels selected for microorganisms with enhanced capabilities related to phosphorus metabolism and heavy metal resistance. This research brings new insights regarding the influence of different phosphate concentrations on mixed-species biofilms formation and drinking water quality, which are relevant to inform best management practices in drinking water treatment.

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Impact of Zinc Orthophosphate on Simulated Drinking Water Biofilms Influenced by Lead and Copper

Cited by 11 publications

References 39 publications

Phosphate Dosing in Drinking Water Distribution Systems Promotes Changes in Biofilm Structure and Functional Genetic Diversity

Phosphate Dosing in Drinking Water Distribution Systems Promotes Changes in Biofilm Structure and Functional Genetic Diversity

Effect of sodium silicate on drinking water biofilm development

Influence of phosphate dosing on biofilms development on lead in chlorinated drinking water bioreactors

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