Impact of Weissella cibaria BYL4.2 and its supernatants on Penicillium chrysogenum metabolism

Abstract

Lactic acid bacteria (LAB) can produce a vast spectrum of antifungal metabolites to inhibit fungal growth. The purpose of this study was to elucidate the antifungal effect of isolated Weissella cibaria BYL4.2 on Penicillium chrysogenum, the antifungal activity of W. cibaria BYL4.2 against P. chrysogenum was evaluated by the superposition method, results showed that it had obviously antifungal activity against P. chrysogenum. Studying the probiotic properties of BYL4.2 and determining it as beneficial bacteria. Furtherly, different treatments were carried out to characterize the antifungal activity of cell-free supernatant (CFS) produced by W. cibaria BYL4.2, and it was shown that the CFS was pH-dependent, partly heat-sensitive, and was not influenced by proteinaceous treatment. The CFS of W. cibaria BYL4.2 was analyzed by high-performance liquid chromatography (HPLC) and found the highest content of lactic acid. Screening of metabolic markers by a non-targeted metabolomics approach based liquid chromatography-mass spectrometry (LC-MS). The results speculated that organic acid especially detected D-tartaric acid was the main antifungal substance of CFS, which could cause the down-regulation of metabolites in the ABC transporters pathway, thereby inhibiting the growth of P. chrysogenum. Therefore, this study may provide important information for the inhibitory mechanism of W. cibaria BYL4.2 on P. chrysogenum, and provide a basis for further research on the antifungal effect of Weissella.

Assessment of safety and in situ antibacterial activity of Weissella cibaria strains isolated from Campos das Vertentes, Minas Gerais, Brazil for their food application

“…Such ability of resilience is directly related to the expression of speci c proteins, pili, mbriae, and agella (Ye et al, 2015). The W. cibaria strains like strain BYL4.2 are also able to inhibit fungi like Penicillium chrysogenum via production of organic acids (Yao et al, 2022). This antifungal activity is relevant in the food sector, as fungi can cause spoilage and deterioration of a variety of food products like dairy products, cereals, fruits and vegetables, and grains, decreasing their quality, and shortening the shelf life, thereby causing considerable economic losses (Le Lay et al, 2016).…”

Genomic insights into Penicillium chrysogenum adaptation to subseafloor sedimentary environments

“…This suggests that P. chrysogenum may possess a wider array of In addition, comparative genome analysis revealed that strain 28R-6-F01 possesses eight unique genes and two expanded gene families related to secondary metabolism, distinguishing it from terrestrial strains (Additional file 2: Table S3, S4). Among these, four ABC transporter genes and three major facilitator superfamily (MFS) coding genes were identified, potentially contributing to penicillin synthesis and multi-drug resistance [34,38,39]. Two unique genes encoding versicolorin reductase were found, suggesting the strain's capability to produce the toxic and carcinogenic mycotoxin aflatoxins [40,41].…”

Assessment of biosecurity traits and in situ antibacterial activities of Weissella cibaria strains isolated from Campos das Vertentes, Minas Gerais, Brazil focused for a potential probiotic and food application

“…Such ability of resilience is directly related to the expression of speci c proteins, pili, mbriae, and agella [48]. The W. cibaria strains like strain BYL4.2 are also able to inhibit fungi like Penicillium chrysogenum via production of organic acids [49]. This antifungal activity is relevant in the food sector, as fungi can cause spoilage and deterioration of a variety of food products like dairy products, cereals, fruits and vegetables, and grains, decreasing their quality of food and shortening the shelf life, thereby causing considerable economic losses [50].…”