Impact of the Melle-Boinot process on the enhancement of second-generation ethanol production by Spathaspora passalidarum

Neitzel, Thiago; Lima, Cleilton Santos; Biazi, Luiz Eduardo; Collograi, Karen Cristina; Costa, Aline Carvalho da; Santos, Leandro Vieira dos; Ienczak, Jaciane Lutz

doi:10.1016/j.renene.2020.07.027

Cited by 23 publications

(15 citation statements)

References 20 publications

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“…On the other hand, Xyl and EtOH did not show significant difference along with batches for SBF with free cells, probably due to discussion previously cited regarding O2 availability in shaker flasks fermentation, and reinforced by Neitzel et al [28] work, where fed-batch fermentations were performed at appropriated O2 supply and an increasing in Xyl, Glu and EtOH were observed along sequential fedbatches.…”

Section: Sbf With Immobilized and Free S Passalidarum Cellsmentioning

confidence: 74%

“…[14] and Neitzel et al [28], respectively. A proportion of 30% glucose and 70% xylose was applied in all fermentations according to Neitzel et al [28], with the aim to simulate sugars content of hemicellulosic hydrolysate obtained from sugarcane bagasse sulfuric acid pre-treatment [29]. Sugar content for each SBF performed is presented in Table 1.…”

Section: Sequential Batch Fermentation With Cell Recyclingmentioning

confidence: 99%

“…They observed that among the five batches with immobilized cells, the best results were obtained in B3, which had a shorter fermentation time (10 h) and a % substrate-toproduct conversion factor the theory stoichiometry, 1.13 times greater than for free cells. Neitzel et al [28] reported improved ethanol production and xylose specific uptake rate by S. passalidarum free cells in fermentations carried out during five repeated fed-batch fermentation with a medium simulating HH in sugars content and proportions.…”

Section: Sbf With Immobilized and Free S Passalidarum Cellsmentioning

confidence: 99%

“…Yield also showed no significant difference and remained in the range of 58.07 ± 3.70 to 65.40 ± 3.68 % for these fermentations. Neitzel et al [28] carried out successive fedbatch fermentation with free cells (initial cell concentration of 90 g/L) of S. passalidarum. They observed significant differences in kinetic parameters along fed-batches, and values for YP/S and QP were 0.39 to 0.46 g/g.L and 1.29 to 1.79 g/L.h for first and fifth fed-batches, respectively [28].…”

Section: Sbf With Immobilized and Free S Passalidarum Cellsmentioning

confidence: 99%

“…Neitzel et al [28] carried out successive fedbatch fermentation with free cells (initial cell concentration of 90 g/L) of S. passalidarum. They observed significant differences in kinetic parameters along fed-batches, and values for YP/S and QP were 0.39 to 0.46 g/g.L and 1.29 to 1.79 g/L.h for first and fifth fed-batches, respectively [28]. In the present work, SBF with immobilized cells in MSC attained the highest YP/S and QP in B2, 0.30 ± 0.02 g/g and 0.81 ± 0.04 g/L.h, significantly different from the values obtained in the other batches (Table 2).…”

Section: Sbf With Immobilized and Free S Passalidarum Cellsmentioning

confidence: 99%

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Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

et al. 2021

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The immobilization of S. passalidarum in calcium alginate beads for second-generation ethanol production (E2G) was evaluated in a medium that simulated a hemicellulosic hydrolysate of sugarcane bagasse pretreated with diluted sulfuric acid in terms of sugars composition. Three sets of sequential batch fermentations (SBF) were carried out with free cells, or immobilized cells in high (HSC) and moderate (MSC) initial sugar concentration (120 and 70 g/L, respectively). SBF were characterized by five consecutive batches performed in shaker, at 30 °C and 110 rpm. Better results were observed for the SBF with immobilized cells in MSC medium when compared to HSC (Y'P/S of 0.27 ± 0.02 and 0.19 ± 0.03 g/g, respectively), in the second batch cycle. The value for YP/S in MSC was similar to the obtained with free cells (0.30 ± 0.02 to 0.33 ± 0.02 g/g). However, QP was lower for MSC, reaching 0.81 ± 0.04 g/L.h in in the second batch, while for free cells the QP varied from 1.06 ± 0.02 to 1.16 ± 0.22 g/L.h. A technique for determining the concentration of immobilized cells in the alginate beads was applied, which made it possible to determine the specific rates for the SBF performed. According to the results obtained, it was possible to demonstrate that S. passalidarum can be immobilized in calcium alginate and reuse through SBF, with performance similar to free cells, which can be a good strategy for fermentation of hemicellulosic hydrolysates.

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Section: Sbf With Immobilized and Free S Passalidarum Cellsmentioning

confidence: 74%

Section: Sequential Batch Fermentation With Cell Recyclingmentioning

confidence: 99%

Section: Sbf With Immobilized and Free S Passalidarum Cellsmentioning

confidence: 99%

Section: Sbf With Immobilized and Free S Passalidarum Cellsmentioning

confidence: 99%

Section: Sbf With Immobilized and Free S Passalidarum Cellsmentioning

confidence: 99%

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Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

et al. 2021

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First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

de Oliveira Pereira,

dos Santos,

Guimarães

et al. 2024

Biotech & Bioengineering

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The integration of first‐ (1G) and second‐generation (2G) ethanol production by adding sugarcane juice or molasses to lignocellulosic hydrolysates offers the possibility to overcome the problem of inhibitors (acetic acid, furfural, hydroxymethylfurfural and phenolic compounds), and add nutrients (such as salts, sugars and nitrogen sources) to the fermentation medium, allowing the production of higher ethanol titers. In this work, an 1G2G production process was developed with hemicellulosic hydrolysate (HH) from a diluted sulfuric acid pretreatment of sugarcane bagasse and sugarcane molasses. The industrial Saccharomyces cerevisiae CAT‐1 was genetically modified for xylose consumption and used for co‐fermentation of sucrose, fructose, glucose, and xylose. The fed‐batch fermentation with high cell density that mimics an industrial fermentation was performed at bench scale fermenter, achieved high volumetric ethanol productivity of 1.59 g L−1 h−1, 0.39 g g−1 of ethanol yield, and 44.5 g L−1 ethanol titer, and shown that the yeast was able to consume all the sugars present in must simultaneously. With the results, it was possible to establish a mass balance for the global process: from pretreatment to the co‐fermentation of molasses and HH, and it was possible to establish an effective integrated process (1G2G) with sugarcane molasses and HH co‐fermentation employing a recombinant yeast.

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Effect of the Sugarcane Bagasse Deacetylation in the Pentoses Fermentation Process

et al. 2021

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Impact of the Melle-Boinot process on the enhancement of second-generation ethanol production by Spathaspora passalidarum

Cited by 23 publications

References 20 publications

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

Effect of the Sugarcane Bagasse Deacetylation in the Pentoses Fermentation Process

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