Effect of the Sugarcane Bagasse Deacetylation in the Pentoses Fermentation Process

Lima, Cleilton Santos; Neitzel, Thiago; Pereira, Isabela de Oliveira; Rabelo, Sarita Cândida; Ienczak, Jaciane Lutz; Roberto, Inês Conceição; Rocha, George J. M.

doi:10.1007/s12155-020-10243-3

Cited by 15 publications

(9 citation statements)

References 64 publications

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“…The content of inhibitors such as acetic acid, furfural, HMF, and formic acid obtained in HH, CHH, and DCHH are presented in Table 1. According to authors from literature (Farias & Filho, 2019; Lima et al, 2021; Milessi, Silva, et al, 2020), H 2 SO 4 sugarcane pretreatments followed by evaporation (CHH) resulted in highest xylose and glucose concentration, in agreement with data presented in Table 1. The comparison of inhibitors presents in HH (Table 1) with that obtained by Lima et al (2021) shown low acetic acid concentration (present in hemicellulose structure and released during pretreatment) for the last one, because it presented an additional step of deacetylation before H 2 SO 4 pretreatment.…”

Section: Resultssupporting

confidence: 87%

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First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

de Oliveira Pereira,

dos Santos,

Guimarães

et al. 2024

Biotech & Bioengineering

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The integration of first‐ (1G) and second‐generation (2G) ethanol production by adding sugarcane juice or molasses to lignocellulosic hydrolysates offers the possibility to overcome the problem of inhibitors (acetic acid, furfural, hydroxymethylfurfural and phenolic compounds), and add nutrients (such as salts, sugars and nitrogen sources) to the fermentation medium, allowing the production of higher ethanol titers. In this work, an 1G2G production process was developed with hemicellulosic hydrolysate (HH) from a diluted sulfuric acid pretreatment of sugarcane bagasse and sugarcane molasses. The industrial Saccharomyces cerevisiae CAT‐1 was genetically modified for xylose consumption and used for co‐fermentation of sucrose, fructose, glucose, and xylose. The fed‐batch fermentation with high cell density that mimics an industrial fermentation was performed at bench scale fermenter, achieved high volumetric ethanol productivity of 1.59 g L−1 h−1, 0.39 g g−1 of ethanol yield, and 44.5 g L−1 ethanol titer, and shown that the yeast was able to consume all the sugars present in must simultaneously. With the results, it was possible to establish a mass balance for the global process: from pretreatment to the co‐fermentation of molasses and HH, and it was possible to establish an effective integrated process (1G2G) with sugarcane molasses and HH co‐fermentation employing a recombinant yeast.

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Section: Resultssupporting

confidence: 87%

“…) if hemicelluloses are considered for secondgeneration production. It is important to emphasize that not only xylose is present in hemicellulosic hydrolysate (HH), but glucose and acetate are also present in this fraction (de Oliveira et al, 2018;Dionísio et al, 2021;Lima et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

de Oliveira Pereira,

dos Santos,

Guimarães

et al. 2024

Biotech & Bioengineering

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“…Yu et al reported corncob hydrolysate with 57.80% glucose and 37.82% xylose. Lima et al . reported sugarcane bagasse hydrolysate with 36.88% glucose and 63.11% xylose.…”

Section: Methodsmentioning

confidence: 99%

Dynamic Modeling Application To Evaluate the Performance of Spathaspora passalidarum in Second-Generation Ethanol Production: Parametric Dynamics and the Likelihood Confidence Region

Martinez-Jimenez

Ribeiro

Sargo

et al. 2021

Ind. Eng. Chem. Res.

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Lignocellulosic materials and agro-industrial residues are essential in a sustainable biorefinery. The effective fermentation of renewable sugars is still an important challenge for an economically viable production of biofuels and biochemicals. This challenge involves biological aspects, such as choosing the microbial platform, and industrial aspects. These aspects can be assessed employing mathematical models to understand the complex synergies between the microorganism and processes. In this work, the performance of the yeast Spathaspora passalidarum was evaluated for the fermentation of xylose and glucose at different proportions, initial concentrations, and initial cell densities using an unstructured−unsegregated kinetic model. The likelihood confidence region, correlations between the parameters, and the convergence of the parameters through the optimization process were identified. It was also possible to identify that π Xyl, max , μ Glu, max , K ' S, Xyl , Y ' PEth/SGlu , Y ' PEth/SXyl , and Y ' X/SGlu were the most representative parameters, due to the high correlation (positive and negative) they have with the other model parameters. It was also possible to identify how initial substrate concentrations affected the specific uptake substrate and ethanol production rates; in this sense, the increase in the glucose concentration causes a decrease in the specific ethanol formation rate at a high concentration of xylose for S. passalidarum cultivated in the conditions proposed by this study.

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“…[14] and Neitzel et al [28], respectively. A proportion of 30% glucose and 70% xylose was applied in all fermentations according to Neitzel et al [28], with the aim to simulate sugars content of hemicellulosic hydrolysate obtained from sugarcane bagasse sulfuric acid pre-treatment [29]. Sugar content for each SBF performed is presented in Table 1.…”

Section: Sequential Batch Fermentation With Cell Recyclingmentioning

confidence: 99%

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

et al. 2021

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The immobilization of S. passalidarum in calcium alginate beads for second-generation ethanol production (E2G) was evaluated in a medium that simulated a hemicellulosic hydrolysate of sugarcane bagasse pretreated with diluted sulfuric acid in terms of sugars composition. Three sets of sequential batch fermentations (SBF) were carried out with free cells, or immobilized cells in high (HSC) and moderate (MSC) initial sugar concentration (120 and 70 g/L, respectively). SBF were characterized by five consecutive batches performed in shaker, at 30 °C and 110 rpm. Better results were observed for the SBF with immobilized cells in MSC medium when compared to HSC (Y'P/S of 0.27 ± 0.02 and 0.19 ± 0.03 g/g, respectively), in the second batch cycle. The value for YP/S in MSC was similar to the obtained with free cells (0.30 ± 0.02 to 0.33 ± 0.02 g/g). However, QP was lower for MSC, reaching 0.81 ± 0.04 g/L.h in in the second batch, while for free cells the QP varied from 1.06 ± 0.02 to 1.16 ± 0.22 g/L.h. A technique for determining the concentration of immobilized cells in the alginate beads was applied, which made it possible to determine the specific rates for the SBF performed. According to the results obtained, it was possible to demonstrate that S. passalidarum can be immobilized in calcium alginate and reuse through SBF, with performance similar to free cells, which can be a good strategy for fermentation of hemicellulosic hydrolysates.

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Effect of the Sugarcane Bagasse Deacetylation in the Pentoses Fermentation Process

Cited by 15 publications

References 64 publications

First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

Dynamic Modeling Application To Evaluate the Performance of Spathaspora passalidarum in Second-Generation Ethanol Production: Parametric Dynamics and the Likelihood Confidence Region

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

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