Impact of the chronic wound microenvironment and marine omega‐3 fatty acids on skin cell regeneration processes

Severing, Anna-Lena; Rembe, Julian‐Dario; Füllerer, Martin; Stürmer, Ewa K.

doi:10.1111/exd.14506

Cited by 3 publications

(1 citation statement)

References 32 publications

(61 reference statements)

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“…Our findings are in accord with a previous study reporting that EPA improves the healing of fibroblast wounds in vitro [ 45 ]. Nevertheless, another study failed to detect differences in keratinocyte and fibroblast migration in cultures treated with EPA or DHA used at a concentration of 150 µM, but it is possible that the higher doses used account for these incongruities [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Topical Administration of a Marine Oil Rich in Pro-Resolving Lipid Mediators Accelerates Wound Healing in Diabetic db/db Mice through Angiogenesis and Macrophage Polarization

Ontoria‐Oviedo

Amaro-Prellezo

Castellano

et al. 2022

IJMS

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Impaired wound healing in patients with type 2 diabetes (DM2) is characterized by chronic inflammation, which delays wound closure. Specialized pro-resolving lipid mediators (SPMs) are bioactive molecules produced from essential polyunsaturated fatty acids (PUFAs), principally omega-3 docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). SPMs are potent regulators of inflammation and have been used to suppress chronic inflammation in peripheral artery disease, non-alcoholic fatty liver disease, and central nervous system syndromes. LIPINOVA® is a commercially available safe-grade nutritional supplement made from a fractionated marine lipid concentrate derived from anchovy and sardine oil that is rich in SPMs and EPA, as well as DHA precursors. Here, we assessed the effect of LIPINOVA® in wound dressing applications. LIPINOVA® showed biocompatibility with keratinocytes and fibroblasts, reduced the abundance of pro-inflammatory macrophages (Mφ1), and promoted in vitro wound closure. Daily application of the marine oil to open wounds made by punch biopsy in db/db mice promoted wound closure by accelerating the resolution of inflammation, inducing neoangiogenesis and Mφ1/Mφ2 macrophage polarization. In conclusion, LIPINOVA® displays pro-resolutive properties and could be exploited as a therapeutic agent for the treatment of diabetic ulcers.

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Section: Discussionmentioning

confidence: 99%

Topical Administration of a Marine Oil Rich in Pro-Resolving Lipid Mediators Accelerates Wound Healing in Diabetic db/db Mice through Angiogenesis and Macrophage Polarization

Ontoria‐Oviedo

Amaro-Prellezo

Castellano

et al. 2022

IJMS

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Development of a Cellular Assay as a Personalized Model for Testing Chronic Wound Therapeutics

Doerfler,

Schoefmann,

Cabral

et al. 2024

Journal of Investigative Dermatology

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Development of carboxymethyl cellulose/gelatin hydrogel loaded with Omega-3 for skin regeneration

Zamani,

Rezaei kolarijani,

Naeiji

et al. 2024

J Biomater Appl

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Hydrogels have several characteristics, including biocompatibility, physical similarity with the skin’s extracellular matrix, and regeneration capacity. Cell migration and proliferation are facilitated by natural polymers such as gelatin (Gel) and carboxymethyl cellulose (CMC). Gelatin dressing acts as a structural framework for cell migration into the wound area, stimulating cell division and promoting granulation tissue formation. Omega-3 fatty acids from fish oil may prevent wound infection and improve the healing of wounds in the early stages. We studied the preparation of wound dressing containing Omega-3 and its ability to heal wounds. In this study, CMC-Gel hydrogels containing different concentrations of Omega-3 were investigated in full-thickness wounds. After the fabrication of the hydrogels by using surfactant (tween 20) and microemulsion method (oil in water), various tests such as SEM, Water uptake evaluation, weight loss, cell viability, blood compatibility, and in vivo study in rat cutaneous modeling during 14 days were performed to evaluate the properties of the fabricated hydrogels. The analysis of the hydrogels revealed that they possess porous structures with interconnected pores, with an average size of 83.23 ± 6.43 μm. The hydrogels exhibited a swelling capacity of up to 60% of their initial weight within 24 h, as indicated by the weight loss and swelling measurements. Cell viability study with the MTT technique showed that no cytotoxicity was observed at the recommended dosage, however, increasing the amount of omega-3 caused hemolysis, cell death, and inhibition of coagulation activity. An in vivo study in adult male rats with a full-thickness model showed greater than 91% improvement of the primary wound region after 2 weeks of treatment. Histological analysis demonstrated Omega-3 in hydrogels, which is a promising approach for topical skin treatment to prevent scar, and has shown efficacy as wound dressing by improving the repair process at the defect site.

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Impact of the chronic wound microenvironment and marine omega‐3 fatty acids on skin cell regeneration processes

Cited by 3 publications

References 32 publications

Topical Administration of a Marine Oil Rich in Pro-Resolving Lipid Mediators Accelerates Wound Healing in Diabetic db/db Mice through Angiogenesis and Macrophage Polarization

Topical Administration of a Marine Oil Rich in Pro-Resolving Lipid Mediators Accelerates Wound Healing in Diabetic db/db Mice through Angiogenesis and Macrophage Polarization

Development of a Cellular Assay as a Personalized Model for Testing Chronic Wound Therapeutics

Development of carboxymethyl cellulose/gelatin hydrogel loaded with Omega-3 for skin regeneration

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