Impact of temperature stress and validamycin A on compatible solutes and fumonisin production in F. verticillioides: Role of trehalose-6-phosphate synthase

Yeast Tolerance to Various Stresses Relies on the Trehalose-6P Synthase (Tps1) Protein, Not on Trehalose

“…The second perspective relies on the urgent need for new antifungal therapies. The trehalose synthase pathway is widespread in the fungal kingdom where it can be part of the virulence system (23,(67)(68)(69), thus making Tps1 a potential antimicrobial target for weakening mammalian and plant pathogenic microorganisms.…”

Analysis of carbohydrates in Fusarium verticillioides using size-exclusion HPLC – DRI and direct analysis in real time ionization – time-of-flight – mass spectrometry (DART-MS)

“…This technique conrmed that disruption of trehalose synthesis through the deletion of the trehalose-6-phosphate synthase gene reduces intracellular trehalose to levels below the detection limit. 35 In addition, we observed that mannitol and arabitol levels in the DTPS1 mutant are less than half of what is present in wild type. This is somewhat surprising in light of the observation that disruption of mannitol biosynthesis in N. scheri leads to increased trehalose levels, suggesting overlapping functions for the two compounds.…”

“…Trehalose levels in the DTPS1 strain were previously shown using an enzymatic assay to be below the limit of detection under all conditions tested. 35 The DRI chromatogram corresponding to the wild-type extract (Fig. 2) is characterized by 3 major peaks eluting around 13, 16, and 17 minutes and numerous minor peaks.…”

“…The M-3125 wild-type strain 39 and DTPS1 mutant strain 35 of F. verticillioides were maintained as spore suspensions in 15% glycerol at À80 C and as mycelia on V8-juice agar at 4 C. Spores were harvested from 7-10 day V8-juice agar cultures and inoculated into GYAM liquid medium (0.24 M glucose, 0.05% yeast extract, 8.0 mM L-asparagine, 5.0 mM malic acid, 1.7 mM NaCl, 4.4 mM K 2 HPO 4 , 2.0 mM MgSO 4 , 8.8 mM CaCl 2 ; pH 3.0) at a ratio of 10 5 spores per mL. equipped with either a 50 mL injection loop for sample analysis or a 200 mL injection loop for fraction collection.…”

Effect of different stress conditions on the vegetative growth of the filamentous fungus Acremonium chrysogenum.

“…Frente a esta condición medioambiental los hongos han desarrollado un mecanismo protector mediante la movilización de solutos compatibles, que conduce a su acumulación. Los solutos compatibles son sustancias orgánicas de bajo peso molecular, altamente solubles, como azúcares, polioles y aminoácidos, empleados por el hongo para balancear la osmolaridad previniendo la perdida de agua (Duran et al, 2010;Boudreau et al, 2013). Está capacidad de osmoadaptación es crucial en las rutas de biosíntesis de metabolitos, siendo el estrés osmótico un factor crítico en la producción de enzimas de interés en fermentaciones industriales (Duran et al, 2010).…”