Analysis of carbohydrates in Fusarium verticillioides using size-exclusion HPLC – DRI and direct analysis in real time ionization – time-of-flight – mass spectrometry (DART-MS)

Abstract

Direct analysis in real time – time of flight – mass spectrometry (DART-TOF-MS) and size-exclusion chromatography (SEC) are used to analyze carbohydrates inF. verticillioides, such as trehalose.

Recent application of direct analysis in real time mass spectrometry in plant materials analysis with emphasis on traditional Chinese herbal medicine

“…The peak at m/z 938.6539 with the highest signal intensity was from the fully methylated ginsenoside ion of [M+14(CH 2 )+N(CH 3 ) 3 +H] + , with the loss of the disaccharide at the C20 position. This approach has also been successfully applied in the detection of timosaponin AIII (Kim et al, 2014), and carbohydrates in Fusarium verticillioides (Roberts et al, 2016).…”

The potency of HPLC-DAD and LC-MS/MS combined with ion chromatography for detection/purification of levulinic acid and bio-compounds from acid hydrolysis of OPEFB

“…Therefore, one pair (1p) in glucose and xylose was eluted rst, and then three pairs (3p) of others followed. 41,43 Since this column uses the size exclusion mode, 44 the elution followed the sequence of disaccharides, monosaccharide C6, and monosaccharide C5, respectively.…”