Impact of Storage Solution Formulation during Refrigerated Storage upon Chondrocyte Viability and Cartilage Matrix

Abstract

Various preservation solutions have been evaluated for longer hypothermic cartilage storage for tissue transplantation; however, the results are mixed. This research was carried out to determine whether phosphate-buffered saline (PBS) or organ preservation solutions would preserve both the extracellular matrix and chondrocytes of articular cartilage better than culture medium during refrigerated storage in the time frame that cartilage is stored for clinical use. Porcine cartilage plugs were stored, without the underlying bone, in culture medium with and without fetal bovine serum (FBS), PBS, Belzer's and Unisol solutions for 1 month at 4°C. Metabolic activity was tested using a resazurin reduction method, and matrix permeability was evaluated by measuring electrical conductivity. Storage in culture medium with 10% FBS was shown to provide good cartilage metabolic function for 7 days, decreasing to about 36% after 1 month of storage. There was no significant difference between samples stored in culture medium with and without FBS after 1 month of storage (p = 0.5005). Refrigerated storage of cartilage in PBS and two different solutions (Belzer's and Unisol) designed for optimal refrigerated tissue and organ storage results in loss of chondrocyte function and retention of matrix permeability. In contrast, the opposite, namely significantly better retention of chondrocyte function and loss of matrix permeability, was observed with culture medium. Future research should be focused on combining retention of chondrocyte function and matrix permeability by storage solution formulation.

Influence of Factors of Cryopreservation and Hypothermic Storage on Survival and Functional Parameters of Multipotent Stromal Cells of Placental Origin

“…As to hypothermic storage, different levels of success can be found in the literature, strongly depended on the cell type and composition of preservation media [ 19 , 32 , 33 ]. In summary, hypothermic storage of cells in suspension causes swelling of cells due to cold inactivation of membrane pumps, as well as activation of cellular stress pathways [ 28 , 34 ] resulting in rapid depletion of cellular energy reserves and loss of stored material.…”

Functional assessment of strains around a full-thickness and critical sized articular cartilage defect under compressive loading using MRI

“…Nevertheless, MRI artefacts caused by a sharp transition between cartilage and surrounding fluid or air bubbles at the articular surface may potentially amplify this effect observed at the defect rim. Furthermore, the use of PBS buffer and storage at 8 C might have affected cell viability especially in the outer layers 31,32,56 . As the current study focused on the acute response of cartilage to loading, the culture conditions were not optimized for long-term experimentation.…”

Banco de piel y tejidos: un modelo operativo para la recuperación y preservación de aloinjertos de piel y tejidos

“…De acuerdo a Wright et al 5 Falt y Marragonni (1959) describieron los primeros procedimientos para el almacenamiento de piel cadavérica mediante el uso de soluciones suplementadas con 10% de suero como agente preservador 5,6 . Todos estos pasos permitieron la creación de un nuevo tipo de establecimientos, capaces de almacenar piel y otros tejidos desde finales de los años cincuenta del siglo pasado.…”