The
unregulated discharge of nanoparticles (NPs) from various nanotechnology
industries into the environment is expected to alter the composition
and physiological functions of soil microbiota. Considering this knowledge
gap, the impact of five NPs (Ag, ZnO, CuO, Al2O3, and TiO2) differing in size and morphology on growth
behavior and physiological activity of Azotobacter
chroococcum, Bacillus thuringiensis, Pseudomonas mosselii, and Sinorhizobium meliloti were investigated. Various
biochemical and microscopic approaches were adopted. Interestingly,
all bacterial strains were found sensitive to Ag-NPs and ZnO-NPs but
showed tolerance toward CuO, Al2O3, and TiO2-NPs. The loss of cellular respiration due to NPs was coupled
with a reduction in population size. ZnO-NPs at 387.5 μg mL–1 had a maximum inhibitory impact on A. chroococcum and reduced its population by 72%.
Under Ag-NP stress, the reduction in IAA secretion by bacterial strains
followed the order S. meliloti (74%)
> P. mosselii (63%) > A. chroococcum (49%). The surface of bacterial cells
had small- or large-sized aggregates of NPs. Also, numerous gaps,
pits, fragmented, and disorganized cell envelopes were visible. Additionally,
a treated cell surface appeared corrugated with depressions and alteration
in cell length and a strong heterogeneity was noticed under atomic
force microscopy (AFM). For instance, NPs induced cell roughness for P. mosselii followed the order 12.6 nm (control)
> 58 nm (Ag-NPs) > 41 nm (ZnO-NPs). TEM analysis showed aberrant
morphology,
cracking, and disruption of the cell envelope with extracellular electron-dense
materials. Increased permeability of the inner cell membrane caused
cell death and lowered EPS production. Ag-NPs and ZnO-NPs also disrupted
the surface adhering ability of bacteria, which varied with time and
concentration of NPs. Conclusively, a plausible mechanism of NP toxicity
to bacteria has been proposed to understand the mechanistic basis
of ecological interaction between NPs and resourceful bacteria. These
results also emphasize to develop strategies for the safe disposal
of NPs.