Impact of rpoB Mutations on Reduced Vancomycin Susceptibility in Staphylococcus aureus

Abstract

Of 38 vancomycin-intermediate

Mechanisms of vancomycin resistance in Staphylococcus aureus

“…In most, but not all, cases, the relevant mutated genes appear to be directly or indirectly involved with the biosynthesis/metabolism of the staphylococcal cell wall, specifically, two-component sensory regulatory systems controlling the transcription of genes in cell wall synthesis (32,33,43). However, mutations in the RNA polymerase gene rpoB were also detected in VISA isolates (42).…”

“…In another study, the predominant genetic change was in the yvqF/vraSR system (41), while Watanabe et al showed that most of the 38 VISA strains recovered from 10 different countries carried a mutated rpoB (42). In most, but not all, cases, the relevant mutated genes appear to be directly or indirectly involved with the biosynthesis/metabolism of the staphylococcal cell wall, specifically, two-component sensory regulatory systems controlling the transcription of genes in cell wall synthesis (32,33,43).…”

Comparison of complete rpoB gene sequence typing and multi-locus sequence typing for phylogenetic analysis of Staphylococcus aureus

“…59 SEONG, KIM, and KWON alternative drug for the treatment of Gram-positive bacteria including S. aureus due to potent bactericidal activity (Sauermann et al, 2008). Recently daptomycinresistant and vancomycin-intermediate resistant S. aureus was reported to possess single nucleotide polymorphisms (SNPs) in rpoB (Cui et al, 2010;Matsuo et al, 2011;Watanabe et al, 2011). In the present study the poultry multi-drug MRSA strain BP11069 carried a unique SNP, E42K, the function of which was unknown.…”

“…The complete rpoB gene sequence (RS) typing is a more economic, informative and alternative method than MLST, and better for subspecies level study (Seong et al, 2012). Some mutations in rpoB confer rifampin, daptomycin and vancomycin resistance in S. aureus (Aubry-Damon et al, 1998;Cui et al, 2010;Watanabe et al, 2011;Wichelhaus et al, 1999). Thus, phylogenetic analysis of S. aureus strains with the complete rpoB gene sequence may provide more information on the resistance to antibiotics which are commonly used in MRSA treatment.…”

Methicillin-resistant Staphylococcus aureus antibiotic resistance and virulence

“…The rpoB mutations selected by rifampicin were found to have multiple resistance to both vancomycin and daptomycin by a possible mechanism of increased cell wall thickness and these mutations always were found in VISA strains (39). Meanwhile, rifampicin-resistant strains were found to contain vancomycin resistance (40), and the researchers suggest the rpoB mutations play an important role in vancomycin resistance. These results may give a reason infectious Staphylococcus aureus strains own resistance to penicillin and its derivatives.…”