Impact of Purified Water Quality on Molecular Biology Experiments

Mabic, Stéphane; Kano, Ichiro

doi:10.1515/cclm.2003.073

Cited by 12 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Initially alginate (Sigma-Aldrich, Sintra, Portugal) (Molecular weight (Mw): 120.000-190.000 Da) and β-TCP (Panreac R , Barcelona, Spain) solutions were prepared. Double deionized and filtered water (milli-Q-water) was obtained using a Milli-Q Advantage A10 ultrapure Water Purification System (resistivity = 18.2 M cm at 25 • C and total organic carbon (TOC): <5 parts per billion; 0.22 μm filter) [19]. Briefly, alginate was dissolved in milli-Q water at 12% (w/v) and the solution was left overnight under magnetic stirring.…”

Section: Preparation Of β-Tcp/alginate Composite Scaffolds By Rapid P...mentioning

confidence: 99%

Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering

et al. 2014

View full text Add to dashboard Cite

The growing need to treat bone-related diseases in an elderly population compels the development of novel bone substitutes to improve patient quality of life. In this context, the advent of affordable and effective rapid prototyping equipment, such as the Fab@home plotter, has contributed to the development of novel scaffolds for bone tissue engineering. In this study, we report for the first time the use of a Fab@home plotter for the production of 3D scaffolds composed by beta-tricalcium phosphate (β-TCP)/alginate hybrid materials. β-TCP/alginate mixtures were used in a proportion of 50/50% (w/w), 30/70% (w/w) and 20/80% (w/w). The printing parameters were optimized to a nozzle diameter of 20 Gauge for the production of rigid scaffolds with pre-defined architectures. We observed that, despite using similar printing parameters, both the precision and resolution of the scaffolds were significantly affected by the blend's viscosity. In particular, we demonstrate that the higher viscosity of 50/50 scaffolds (150.0 ± 3.91 mPa s) provides a higher precision in the extrusion process. The physicochemical and biological characterization of the samples demonstrated that the 50/50 scaffolds possessed a resistance to compression comparable to that of native trabecular bone. Moreover, this particular formulation also exhibited a Young's modulus that was higher than that of trabecular bone. Scanning electron microscopy and fluorescence microscopy analysis revealed that osteoblasts were able to adhere, proliferate and also penetrate into the scaffold's architecture. Altogether, our findings suggest that the Fab@home printer can be employed in the manufacture of reproducible scaffolds, using a formulation 50/50 alginate-β-TCP that has suitable properties to be applied as bone substitutes in the future.

show abstract

Section: Preparation Of β-Tcp/alginate Composite Scaffolds By Rapid P...mentioning

confidence: 99%

Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Os íons, como fosfato e muitos outros bivalentes, também têm capacidade de se ligar ao DNA e ao RNA das amostras, interferindo nas análises proteicas (8) . É fundamental fazer a manutenção correta dos equipamentos responsáveis pela purificação da água, minimizando-se a contaminação microbiana (18) .…”

Section: Discussionunclassified

“…• deionização -é utilizada para remoção de substâncias inorgânicas, empregando-se colunas com resinas carregadas eletricamente, que permitem a troca seletiva de íons por compostos inorgânicos dissolvidos na água (5,6,17,18) ;…”

Section: Tecnologias De Purificaçãounclassified

See 1 more Smart Citation

A importância da qualidade da água reagente no laboratório clínico

Mendes¹,

Fagundes²,

Porto³

et al. 2011

J. Bras. Patol. Med. Lab.

View full text Add to dashboard Cite

show abstract

“…Moreover, a total organic carbon (TOC) level <30 p.p.b. is recommended, as organic components are known to interact with the alkyl groups grafted on the column, thereby leading to loss of resolution (29). Lastly, a reliable multiplex PCR is obviously a key step for a successful MP/LC analysis.…”

Section: Mp/lc Design and Performancementioning

confidence: 99%

Multiplex PCR/liquid chromatography assay for detection of gene rearrangements: application to RB1 gene

Dehainault

Laugé²,

Caux‐Moncoutier³

et al. 2004

Nucleic Acids Research

View full text Add to dashboard Cite

Screening for large gene rearrangements is established as an important part of molecular medicine but is also challenging. A variety of robust methods can detect whole-gene deletions, but will fail to detect more subtle rearrangements that may involve a single exon. In this paper, we describe a new, versatile and robust method to assess exon copy number, called multiplex PCR/liquid chromatography assay (MP/LC). Multiple exons are amplified using unlabeled primers, then separated by ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC), and quantitated by fluorescent detection using a post-column intercalation dye. The relative peak intensities for each target directly reflect exon copy number. This novel technique was used to screen a panel of 121 unrelated retinoblastoma patients who were tested previously using a reference strategy. MP/LC correctly scored all deletions and demonstrated a previously undetected RB1 duplication, the first to be described. MP/LC appears to be an easy, versatile, and cost-effective method, which is particularly relevant to denaturing HPLC (DHPLC) users since it broadens the spectrum of available applications on a DHPLC system.

show abstract

Impact of Purified Water Quality on Molecular Biology Experiments

Cited by 12 publications

References 10 publications

Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering

Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering

A importância da qualidade da água reagente no laboratório clínico

Multiplex PCR/liquid chromatography assay for detection of gene rearrangements: application to RB1 gene

Contact Info

Product

Resources

About