Impact of physical parameters on particle size and reaction yield when using the ionic gelation method to obtain cationic polymeric chitosan–tripolyphosphate nanoparticles

Fàbregas, Anna; Carmona, Montserrat Miñarro; García-Montoya, Encarna; Pérez-Lozano, Pilar; Carrillo, Carolina; Sarrate, Rocío; Sánchez-Izquierdo, N.; Grau, Josep R. Ticó; Suñé-Negre, Josep M.

doi:10.1016/j.ijpharm.2013.02.015

Cited by 86 publications

(51 citation statements)

References 17 publications

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“…30,31 The adjustment of parameters such as CS concentration, the CS:TPP polymer ratio, and mixing conditions control CS-TPP NP size. [32][33][34] Initial studies aiming to optimize NP formation were conducted. Particle size and particle size and polydispersity were measured with a Zetasizer Nano.…”

Section: Preparation Of Cs-tpp Npsmentioning

confidence: 99%

Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

Servat-Medina¹,

González-Gómez²,

Reyes-Ortega³

et al. 2015

IJN

100

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Natural products using plants have received considerable attention because of their potential to treat various diseases. Arrabidaea chica (Humb. & Bonpl.) B. Verlot is a native tropical American vine with healing properties employed in folk medicine for wound healing, inflammation, and gastrointestinal colic. Applying nanotechnology to plant extracts has revealed an advantageous strategy for herbal drugs considering the numerous features that nanostructured systems offer, including solubility, bioavailability, and pharmacological activity enhancement. The present study reports the preparation and characterization of chitosan–sodium tripolyphosphate nanoparticles (NPs) charged with A. chica standardized extract (AcE). Particle size and zeta potential were measured using a Zetasizer Nano ZS. The NP morphological characteristics were observed using scanning electron microscopy. Our studies indicated that the chitosan/sodium tripolyphosphate mass ratio of 5 and volume ratio of 10 were found to be the best condition to achieve the lowest NP sizes, with an average hydrodynamic diameter of 150±13 nm and a zeta potential of +45±2 mV. Particle size decreased with AcE addition (60±10.2 nm), suggesting an interaction between the extract’s composition and polymers. The NP biocompatibility was evaluated using human skin fibroblasts. AcE-NP demonstrated capability of maintaining cell viability at the lowest concentrations tested, stimulating cell proliferation at higher concentrations. Antiulcerogenic activity of AcE-NP was also evaluated with an acute gastric ulcer experimental model induced by ethanol and indomethacin. NPs loaded with A. chica extract reduced the ulcerative lesion index using lower doses compared with the free extract, suggesting that extract encapsulation in chitosan NPs allowed for a dose reduction for a gastroprotective effect. The AcE encapsulation offers an approach for further application of the A. chica extract that could be considered a potential candidate for ulcer-healing pharmaceutical systems.

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Section: Preparation Of Cs-tpp Npsmentioning

confidence: 99%

Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

Servat-Medina¹,

González-Gómez²,

Reyes-Ortega³

et al. 2015

IJN

100

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“…These nanoparticles can achieve a size of up to 50 nm and a positive surface charge (reported as ζ potential) between +20 mV and +60 mV [13]. Chitosantripolyphosphate nanoparticles are intended for in vivo administration, so that no harmful residue exists that may compromise the safety of the organism to which they are delivered.…”

Section: Design Of Chitosan Nanoparticles For Synergistic Antimicrobimentioning

confidence: 99%

“…Determination of particle size (apparent hydrodynamic diameter) was performed by dynamic light scattering (DLS), polydispersity index value (size distribution) and surface electric charge, reported as ζ potential or electrophoretic mobility [13].…”

Section: Determination Of Particle Size and ζ Potentialmentioning

confidence: 99%

“…The AFM probes used for this study were rectangular silicon probes with a nominal spring constant of 40 nN/nm. Similarly, image visualization was carried out in a scanning electron microscope (SEM) Hitachi S-3700 with a 15 nm gold coating on the diluted samples (1/10) using a aluminum base at an acceleration voltage of 15 kV [13].…”

Section: Morphology Analysis Of Chitosan Nanoparticlesmentioning

confidence: 99%

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Evaluation of the Synergistic Effect of EDTA-Functionalized Chitosan Nanoparticles on Imipenem Delivery in <i>Pseudomonas aeruginosa</i> Carbapenem-Resistant Strain AG1

Porras-Gómez¹,

Vega-Baudrit²,

García³

et al. 2018

JBNB

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Metallo-β-lactamases are bacterial zinc-dependent enzymes involved in the hydrolysis of β-lactamic antibiotics representing the main cause of bacterial resistance to carbapenems, drugs of last resort for treating infections caused by multiresistant bacteria. We elaborated the hypothesis that it is possible to inhibit the enzymatic activity of metallo-β-lactamases by lowering the availability of zinc in the extracellular medium using metal chelating agents such as EDTA carried on nanoparticles. Chitosan, as linear cationic polysaccharide is frequently used in biomedical and pharmaceutical applications, has been studied as a biocompatible encapsulating agent in drug delivery systems and is an ideal transport agent for bioactive molecular complexes in antibiotic applications due to its ability to associate with negatively charged substances. We developed novel nanoparticles using chitosan as a transport matrix for β-lactamic antibiotics. Nanoparticles were synthesized according to the ion gelation method using tripolyphosphate as crosslinking agent. Nanoparticles were functionalized by the adsorption of EDTA, which acts as complexifying agent for Zn 2+ ions causing inhibition of metallo-β-lactamases activity. We evaluate the antimicrobial effects of EDTA-functionalized nanoparticles with an imipenem cargo on the clinical isolate P. aeruginosa AG1, a carbapenem-resistant high-risk clone ST-111 carrying both bla and bla VIM-2 metallo-β-lactamases genes.

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“…Berdasarkan ukuran tersebut, sampel larutan pada penelitian ini telah berbentuk nanopartikel. Bentuk ukuran partikel banyak dipengaruhi oleh beberapa faktor diantaranya konsentrasi TPP, konsentrasi larutan kitosan, rasio volume larutan kitosan dan TPP, lama pengadukan, kecepatan pengadukan, dan lamanya waktu penyimpanan (Calvo et al,1997;Mardliyati dkk., 2012;Fabregas et al, 2013;Supitjah et al, 2011).…”

Section: Metodeunclassified

Sintesis dan Aplikasi Nanopartikel Kitosan Sebagai Adsorben Cd dan Antibakteri Koliform

Yudhasasmita

Nugroho

2017

Biogenesis

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Chitosan is a derivative compound of chitin which has a linear polysaccharide composed of β-(1-4)-linked d-glucosamine and N-acetyl-d-glucosamine. This compound is found in shrimp shell. In nanoparticle form, chitosan has a great antibacterial activity and adsorption ability rather than normal form. The aims of this study are to study the effect of concentration chitosan nanoparticle to adsorpt cadmium and its antibacterial effect on coliform. This research was started with synthesis of chitosan nanoparticles using acetic acid 2% and TPP 0,1 %, then the sampel was dried by spray dryer. For cadmium adsorption test was conducted by giving chitosan nanoparticle of 0.1, 0.2, 0.3, 0.4, and 0.5 g, respectively, into Cd solution with the concentration of 7 ppm and was filtered with theWhatman paper number 42 (n=3). Cadmium contents in the filtrates and pellets were analyzed by using AAS. For antibacterial test with Salmonella typhimurium andEscherichia coliwere conducted by disc diffusion method, contained of 0.1, 0.2, 0.3, 0.4, and 0.5 g, respectively, were dissolved in 50 ml of 1% acetic acid. Data were analyzed by using oneway ANOVA followed by LSD. Based on the results, the optimum adsorption of cadmium present in addition of 0.4 g/ 50mL, can reduce Cd concentration by 98,7%. For the antibacterial test, the addition of 0.4 g/50mL is the largest zone of inhibition Salmonella thypimuriumand Escherichia coli.Keywords: antibacterial coliform, cadmium adsorbent, chitosan nanoparticle INTISARI Kitosan merupakan senyawa derivat dari kitin yang memiliki linear polisakarida yang tersusun dari β-(1-4)-linked d-glucosamine dan N-acetyl-d-glucosamine. Senyawa ini banyak terkandung dalam kulit udang. Dalam bentuk nanopartikel, kitosan memiliki aktivitas antibakteri dan adsorpsi yang lebih baik jika dibandingkan dalam bentuk biasa. Tujuan penelitian ini adalah untuk mempelajari pengaruh konsentrasi kitosan nanopartikel terhadap pengikatan cadmium (Cd) dan efeknya pada aktivitas antibakteri koliform. Penelitian ini dimulai dengan sintesis senyawa nanopartikel kitosan dengan larutan asam cuka 2% dan TPP 0,1, kemudian larutan dikeringkan dengan spray drier. Uji adsorpsi logam cadmium dilakukan dengan menambahkan 0,1; 0,2; 0,3; 0,4 dan 0,5 gram nanopartikel kitosan ke dalam larutan cadmium dengan konsentrasi 7 ppm,dan disaring dengan kertas Whatman no 42 (n=3). Kandungan Cd dalam filtrat dan residu ditentukan dengan AAS. Uji antibakteri terhadap Salmonella typhimurium dan Escherichia coli dilakukan dengan metode cakram, yang mengandung 0,1; 0,2; 0,3; 0,4 dan 0,5 gram nanopartikel kitosan dalam 50 ml asam cuka 1%. Data kemudian dianalisis dengan oneway ANOVA dilanjutkan dengan LSD, apabila ditemukan beda nyata. Hasil yang diperoleh menunjukkan bahwa pengikatan optimum Cd terjadi pada penambahan kitosan sebanyak 0,4 g/50 mL asam cuka, dengan penurunan sebesar 98,7%. Pada uji antibakteri, penambahan kitosan 0,4 g/50 mL asam cuka menunjukkan penghambatan tertinggi terhadap pertumbuhan S. typhimurium dan E. coli.Kata kunci: adsor...

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Impact of physical parameters on particle size and reaction yield when using the ionic gelation method to obtain cationic polymeric chitosan–tripolyphosphate nanoparticles

Cited by 86 publications

References 17 publications

Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

Evaluation of the Synergistic Effect of EDTA-Functionalized Chitosan Nanoparticles on Imipenem Delivery in <i>Pseudomonas aeruginosa</i> Carbapenem-Resistant Strain AG1

Sintesis dan Aplikasi Nanopartikel Kitosan Sebagai Adsorben Cd dan Antibakteri Koliform

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Impact of physical parameters on particle size and reaction yield when using the ionic gelation method to obtain cationic polymeric chitosan–tripolyphosphate nanoparticles

Cited by 86 publications

References 17 publications

Chitosan&ndash;tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and&nbsp;antiulcerogenic activity

Chitosan&ndash;tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and&nbsp;antiulcerogenic activity

Evaluation of the Synergistic Effect of EDTA-Functionalized Chitosan Nanoparticles on Imipenem Delivery in &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; Carbapenem-Resistant Strain AG1

Sintesis dan Aplikasi Nanopartikel Kitosan Sebagai Adsorben Cd dan Antibakteri Koliform

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Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

Evaluation of the Synergistic Effect of EDTA-Functionalized Chitosan Nanoparticles on Imipenem Delivery in <i>Pseudomonas aeruginosa</i> Carbapenem-Resistant Strain AG1