Impact of pharmaceutical products on zebrafish: An effective tool to assess aquatic pollution

Porretti, Miriam; Arrigo, Federica; Bella, Giuseppa Di; Faggio, Caterina

doi:10.1016/j.cbpc.2022.109439

Cited by 28 publications

(10 citation statements)

References 40 publications

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“…Particularly, their validity to reflect the impact of micropollutants, like pharmaceuticals, has been proven in recent years ( Faggio et al, 2018 ; Piedade et al, 2020 ; Martyniuk et al, 2022a ; Martyniuk et al, 2022b ; Martyniuk et al, 2023 ; Chahouri et al, 2023 ; Impellitteri et al, 2023a ; Impellitteri et al, 2023b ). The pharmaceuticals represent a comparatively novel group of micropollutants of increasing concern given their widely discharged into water bodies on a continual basis ( Bottoni et al, 2010 ; Fabbri and Franzellitti, 2016 ; Turani et al, 2019 ; Martyniuk et al, 2023 ; Porretti et al, 2022 ). They fall into the water along with the insufficiently purified sewage from water treatment plants and directly from households in rural areas, which often lack wastewater treatment facilities ( Burgos-Aceves et al, 2018 ; Burgos-Aceves et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Cellular and oxidative stress responses of Mytilus galloprovincialis to chlorpromazine: implications of an antipsychotic drug exposure study

Impellitteri,

Yunko,

Martyniuk

et al. 2023

Front. Physiol.

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Introduction: Bivalve molluscs like Mytilus galloprovincialis are valuable bioindicators due to their filter-feeding lifestyle, wide distribution, and ability to concentrate xenobiotics. Studying the effects of pharmaceuticals on these molluscs is crucial given their presence in surface waters. This study investigated the response of M. galloprovincialis to chlorpromazine (Cpz), an antipsychotic with antiviral activity against influenza, HIV, and coronaviruses in human cells.Methods: In this study, we examined the 14-day impact of chlorpromazine (Cpz) on the model species M. galloprovincialis at two concentrations (Cpz 1: 12 ng L-1 or 37 pM; Cpz 2: 12 µg L-1 or 37 nM). To ensure controlled exposure, a stock solution of Cpz was prepared and introduced into the tanks to match the intended concentrations. Seawater and stock solutions were refreshed every 48 h. The primary focus of this study centered on evaluating cell viability, cell volume regulation, and oxidative stress indicators.Results: Although cell volume regulation, as assessed by decreasing regulatory volume Regulation volume decrease, did not show statistically significant changes during the experiment, digestive cell viability, on the other hand, showed a significant decrease (p < 0.01) in the Cpz 2 group, suggesting effects on the general health and survival of these cells. Biochemically, in both Cpz 1 and Cpz 2, superoxide dismutase activity increased, while catalase (CAT) decreased, causing an elevated lipid peroxidation thiobarbituric acid-reactive substances and protein carbonyls, particularly in the Cpz 2 group. The level of reduced glutathione (GSH) increased in both exposures, whereas the level of GSSG increased only in the Cpz 1 group. Consequently, the GSH/GSSG ratio was elevated in the Cpz 2 group only.Discussion: A comparison of the magnitudes of anti- and pro-oxidative manifestations indicated a pro-oxidative shift in both exposures. These findings show that Cpz induces non-specific symptoms of biochemical and cellular disturbances in M. galloprovincialis even at the low picomolar concentration.

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Section: Introductionmentioning

confidence: 99%

Cellular and oxidative stress responses of Mytilus galloprovincialis to chlorpromazine: implications of an antipsychotic drug exposure study

Impellitteri,

Yunko,

Martyniuk

et al. 2023

Front. Physiol.

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“…Although, with this concentration, the therapeutic effects of these pharmaceutical compounds are negligible, and bioaccumulation, chronic toxicity, and mutagenicity of their pharmacologically active components in water systems pose potential hazards to aquatic life, the environment, and human health. In fact, long-term exposure to lowconcentrated antibiotics, nonsteroid anti-inflammatory drugs (NSAIDs), antihypertensive drugs, and antidepressants has been demonstrated to affect the cardiovascular and cardiac irregularities in zebrafish [50,51] and water fleas (Daphnia magna) [52], as well as hypoglycemia, growth inhibition, and sexual development alteration in aquatic organisms [2,53]. In addition, Rodríguez et al evaluated the toxicity and risk of pharmaceuticals present in wastewater and reported highly hazardous pharmaceuticals detected in the hospital wastewater samples, such as azithromycin, clarithromycin, doxycycline, cephalexin, ciprofloxacin, and ofloxacin [54].…”

Section: Pharmaceuticals In the Environmentmentioning

confidence: 99%

Recent Advances in Advanced Oxidation Processes for Degrading Pharmaceuticals in Wastewater—A Review

Roslan,

Lau,

Suhaimi

et al. 2024

Catalysts

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A large variety of pharmaceutical compounds have recently been detected in wastewater and natural water systems. This review highlighted the significance of removing pharmaceutical compounds, which are considered indispensable emerging contaminants, from wastewater and natural water systems. Various advanced oxidation processes (AOPs), including UV-H2O2, Fenton and photo-Fenton, ozone-based processes, photocatalysis, and physical processes, such as sonolysis, microwave, and electron beam irradiation, which are regarded as the most viable methods to eliminate different categories of pharmaceutical compounds, are discussed. All these AOPs exhibit great promising techniques, and the catalytic degradation process of the emerging contaminants, advantages, and disadvantages of each technique were deliberated. Heterogeneous photocatalysis employing metal oxides, particularly anatase TiO2 nanoparticles as catalysts activated by UV light irradiation, was reviewed in terms of the electron–hole separation, migration of the charge carriers to the catalyst surfaces, and redox potential of the charge carriers. This brief overview also emphasized that anatase TiO2 nanoparticles and TiO2-based nanomaterials are promising photocatalysts, and a combination of photocatalysis and other AOPs enhanced photocatalytic degradation efficiency. Finally, the challenges of applying anatase TiO2-based photocatalysis in environmental remediation and wastewater treatments to degrade pharmaceutical compounds, including mass spectroscopic analysis and a biological activity test of by-products of the emerging contaminants resulting from photocatalysis, are summarized.

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“…The overuse of PhACs and unsuccessful wastewater treatment has led to the accumulation of this group of chemicals in aquatic systems, which has been reflected in behavioral and physiological changes in the animals [220]. Pharmaceuticals occurring in the water affect not only fish organ development and function but also their DNA, hormone secretion and others [221].…”

Section: Pharmaceuticalmentioning

confidence: 99%

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

Gałązka¹,

Jankiewicz²,

Szczepkowski³

2023

Preprint

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Industrialization, intensive farming, rapid population growth and urbanization are the source of a large number of pollutants entering the environment. The current concentration of xenobiotics released into the environment exceeds its natural ability to decompose them. Enzymatic degradation of pollutants seems to be an environmentally friendly process. Due to the wide spectrum of substrate specificity, from inorganic compounds to high molecular weight organic compounds such as PAH or dyes, as well as favorable biochemical properties, laccase has been used in the biological removal of xenobiotics from the environment. It is important to understand the degradation mechanisms of pollutants and to evaluate the final products in terms of their toxicity. The laccase oxidizes the substrates with the simultaneous reduction of molecular oxygen to water, which is the purest reaction co-substrate. That is why it is called a green biocatalyst. The trend is an increase in the production of enzymes related to the intensive development of industry, bioremediation or synthetic chemistry. This leads to the search for laccases with greater activity and stability under extreme conditions. The potential of laccases to degrade xenobiotics can be promoted by improving enzymatic catalytic characterization using protein engineering and other genetic engineering methods.

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Impact of pharmaceutical products on zebrafish: An effective tool to assess aquatic pollution

Cited by 28 publications

References 40 publications

Cellular and oxidative stress responses of Mytilus galloprovincialis to chlorpromazine: implications of an antipsychotic drug exposure study

Cellular and oxidative stress responses of Mytilus galloprovincialis to chlorpromazine: implications of an antipsychotic drug exposure study

Recent Advances in Advanced Oxidation Processes for Degrading Pharmaceuticals in Wastewater—A Review

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

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