Impact of Phanerochaete chrysosporium inoculation on indigenous bacterial communities during agricultural waste composting

Abstract

This research was conducted to distinguish between the separate effects of the Phanerochaete chrysosporium inoculation and sample property heterogeneity induced by different inoculation regimes on the indigenous bacterial communities during agricultural waste composting. P. chrysosporium was inoculated during different phases. The bacterial community abundance and structure were determined by quantitative PCR and denaturing gradient gel electrophoresis analysis, respectively. Results indicated a significant stimulatory effect of P. chrysosporium inoculation on the bacterial community abundance. The bacterial community abundance significantly coincided with pile temperature, ammonium, and nitrate (P<0.006). Variance partition analysis showed that the P. chrysosporium inoculation directly explained 20.5% (P=0.048) of the variation in the bacterial communities, whereas the sample property changes induced by different inoculation regimes indirectly explained up to 35.1% (P=0.002). The bacterial community structure was significantly related to pile temperature, water-soluble carbon (WSC), and C/N ratio when P. chrysosporium were inoculated. The C/N ratio solely explained 7.9% (P=0.03) of the variation in community structure, whereas pile temperature and WSC explained 7.7% (P=0.026) and 7.5% (P=0.034) of the variation, respectively. P. chrysosporium inoculation affected the indigenous bacterial communities most probably indirectly through increasing pile temperature, enhancing the substrate utilizability, and changing other physico-chemical factors.

Fungal Pretreatment of Effluent From Piggery Anaerobic Digestion by Phanerochaete chrysosporium

“…This phenomenon can be explained as the expression behavior of MnP genes while fungi utilize glucose as a major carbon source in synthetic media [30]. It should be noted that COD reduction at high levels was consistent with the previous study [21], which might be due to selective effects of P. chrysosporium on the indigenous bacterial communities [31]. Phanerochaete chrysosporium changed the effluent properties (e.g., pH and C/N ratio) which could induce significant modifications in the bacterial communities and also provide favorable conditions for bacteria such as optimal temperature [32].…”

Potential microbial drivers of biodegradation of polycyclic aromatic hydrocarbons in crude oil sludge using a composting technique

“…Certainly, production of enzymes by both bacteria and fungi with complementary biodegradation attributes provides a platform for complete biodegradation of PAHs in natural environments. However, this is not always the case as some other important factors may influence the biodegradation process directly or indirectly . These factors are the substrate uptake capability of the microbes, as well as sorption and transmembrane processes.…”

“…However, this is not always the case as some other important factors may influence the biodegradation process directly or indirectly. 22,23 These factors are the substrate uptake capability of the microbes, as well as sorption and transmembrane processes. Furthermore, the ability of microbial isolates to produce suitable enzymes for the metabolism of HMW PAHs could be hindered by low bioavailability of PAHs.…”

Diversity of Two-Domain Laccase-Like Multicopper Oxidase Genes in Streptomyces spp.: Identification of Genes Potentially Involved in Extracellular Activities and Lignocellulose Degradation during Composting of Agricultural Waste

“…Hemicellulose content was assessed by the difference between NDF and ADF, cellulose content by the difference between ADF and ADL, and lignin content by the difference between ADL and ash content. This detergent-fiber analytical method was used because of its convenience and consistency for agricultural wastes (28,29), although the outcome might be slightly biased by proteins bound to lignin or by a contaminant, particularly cutin, present in the acidinsoluble residues.…”