Diversity of Two-Domain Laccase-Like Multicopper Oxidase Genes in Streptomyces spp.: Identification of Genes Potentially Involved in Extracellular Activities and Lignocellulose Degradation during Composting of Agricultural Waste

Lu, Lunhui; Zeng, Guangming; Fan, Changzheng; Zhang, Jiachao; Chen, Anwei; Chen, Ming; Jiang, Min; Yuan, Yujie; Wu, Haipeng; Lai, Mingyong; He, Yiming

doi:10.1128/aem.00223-14

Cited by 45 publications

(24 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…, Lu et al. ). However, this expanded bacterial role in lignin‐decay has apparently not offset reductions in fungal lignin decay, given the reductions in lignin decay occurring under experimental N deposition in our field experiment.…”

Section: Discussionmentioning

confidence: 99%

Anthropogenic N deposition increases soil C storage by reducing the relative abundance of lignolytic fungi

Entwistle

Zak

Argiroff

2018

Ecological Monographs

View full text Add to dashboard Cite

Atmospheric nitrogen (N) deposition has increased dramatically since preindustrial times and continues to increase across many regions of the Earth. In temperate forests, this agent of global change has increased soil carbon (C) storage, but the mechanisms underlying this response are not understood. One long‐standing hypothesis proposed to explain the accumulation of soil C proposes that higher inorganic N availability may suppress both the activity and abundance of fungi that decay lignin and other polyphenols in soil. In field studies, elevated rates of N deposition have reduced the activity of enzymes mediating lignin decay, but a decline in the abundance of lignolytic fungi has not been definitively documented to date. Here, we tested the hypothesis that elevated rates of anthropogenic N deposition reduce the abundance of lignolytic fungi. We conducted a field experiment in which we compared fungal communities colonizing low‐lignin, high‐lignin, and wood substrates in a northern hardwood forest that is part of a long‐term N deposition experiment. We reasoned that if lignolytic fungi decline under experimental N deposition, this effect should be most evident among fungi colonizing high‐lignin and wood substrates. Using molecular approaches, we provide evidence that anthropogenic N deposition reduces the relative abundance of lignolytic fungi on both wood and a high‐lignin substrate. Furthermore, experimental N deposition increased total fungal abundance on a low‐lignin substrate, reduced fungal abundance on wood, and had no significant effect on fungal abundance on a high‐lignin substrate. We simultaneously examined these responses in the surrounding soil and forest floor, in which we did not observe significant reductions in the relative abundance of lignolytic fungi or in the size of the fungal community; however, we did detect a change in community composition in the forest floor that appears to be driven by a shift away from lignolytic fungi and towards cellulolytic fungi. Our results provide direct evidence that reductions in the abundance of lignolytic fungi are part of the mechanism by which anthropogenic N deposition increases soil C storage.

show abstract

Section: Discussionmentioning

confidence: 99%

Anthropogenic N deposition increases soil C storage by reducing the relative abundance of lignolytic fungi

Entwistle

Zak

Argiroff

2018

Ecological Monographs

View full text Add to dashboard Cite

show abstract

“…The 6 class B enzymes are from soil bacteria implicated in biomass degradation, and recent work has suggested that the Streptomyces 2dMCOs participate in lignocellulose degradation. [40] The handful of reported formal potentials for the type 1 sites in the 2dMCOs are below 400 mV vs. NHE ( S. viridochromogenes , 350; [41] S. sviceus , 375 mV; [42] S. coelicolor , 378 mV; [43] ). Given their low potentials, the ability of these bacterial 2dMCOs to oxidize phenolic and lignocellulosic substrates at the type 1 copper site is remarkable, particularly in comparison to heme peroxidases that are thought to employ Trp radicals in lignin degradation.…”

Section: Tyr/trp Chains In Two-domain Multicopper Oxidasesmentioning

confidence: 99%

“…ATCC 39116 (PDB ID 3T9W); [40a] (b) Streptomyces viridosporus (3TAS); [40a] (c) Streptomyces viridochromogenes (4N8U); (d) Streptomyces sviceus (4M3H); [42] (e) Streptomyces lividans (4GYB); (f) Streptomyces coelicolor (3KW8); [46b, 82] (g) Nitrosomonas europaea (3G5W); [44] (h) Arthrobacter sp. FB24 (3GDC).…”

Section: Figurementioning

confidence: 99%

The Rise of Radicals in Bioinorganic Chemistry

Gray

2016

Israel Journal of Chemistry

View full text Add to dashboard Cite

Prior to 1950, the consensus was that biological transformations occurred in two-electron steps, thereby avoiding the generation of free radicals. Dramatic advances in spectroscopy, biochemistry, and molecular biology have led to the realization that protein-based radicals participate in a vast array of vital biological mechanisms. Redox processes involving high-potential intermediates formed in reactions with O2 are particularly susceptible to radical formation. Clusters of tyrosine (Tyr) and tryptophan (Trp) residues have been found in many O2-reactive enzymes, raising the possibility that they play an antioxidant protective role. In blue copper proteins with plastocyanin-like domains, Tyr/Trp clusters are uncommon in the low-potential single-domain electron-transfer proteins and in the two-domain copper nitrite reductases. The two-domain muticopper oxidases, however, exhibit clusters of Tyr and Trp residues near the trinuclear copper active site where O2 is reduced. These clusters may play a protective role to ensure that reactive oxygen species are not liberated during O2 reduction.

show abstract

“…All experiments were done in triplicate. Serial 10-fold plasmid dilutions of plasmids DNA from 1.0×10 4 to 1.0×10 11 copies were acted as templates for standard curve generation (Lu et al 2014). …”

Section: Qpcrmentioning

confidence: 99%

Response of rhizosphere microbial community structure and diversity to heavy metal co-pollution in arable soil

Deng

Zeng

Fan

et al. 2015

Appl Microbiol Biotechnol

Self Cite

127

View full text Add to dashboard Cite

Due to the emerging environmental issues related to heavy metals, concern about the soil quality of farming lands near manufacturing district is increasing. Investigating the function of soil microorganisms exposed to long-term heavy metal contamination is meaningful and important for agricultural soil utilization. This article studied the potential influence of several heavy metals on microbial biomass, activity, abundance, and community composition in arable soil near industrial estate in Zhuzhou, Hunan province, China. The results showed that soil organic contents (SOC) were significantly positive correlated with heavy metals, whereas dehydrogenase activity (DHA) was greatly depressed by the heavy metal stress. Negative correlation was found between heavy metals and basal soil respiration (BSR), and no correlation was found between heavy metals and microbial biomass content (MBC). The quantitative PCR (QPCR) and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis could suggest that heavy metal pollution has significantly decreased abundance of bacteria and fungi and also changed their community structure. The results could contribute to evaluate heavy metal pollution level in soil. By combining different environmental parameters, it would promote the better understanding of heavy metal effect on the size, structure, and activity of microbial community in arable soil.

show abstract

Diversity of Two-Domain Laccase-Like Multicopper Oxidase Genes in Streptomyces spp.: Identification of Genes Potentially Involved in Extracellular Activities and Lignocellulose Degradation during Composting of Agricultural Waste

Cited by 45 publications

References 44 publications

Anthropogenic N deposition increases soil C storage by reducing the relative abundance of lignolytic fungi

Anthropogenic N deposition increases soil C storage by reducing the relative abundance of lignolytic fungi

The Rise of Radicals in Bioinorganic Chemistry

Response of rhizosphere microbial community structure and diversity to heavy metal co-pollution in arable soil

Contact Info

Product

Resources

About